期刊论文

Valproic acid combined with ATRA induces cervical cancer cells differentiation in three-dimensional cell culture model

中文

现代妇产科进展

Progress in Obstetrics and Gynecology

1004-7379

2014

23

3

183-187

10.13283/j.cnki.xdfckjz.2014.03.006

国家自然科学基金(No:81372779,81372777)； 安徽省自然科学基金(No:110406M176,110406M178)；

本校为其他机构

目的:探讨丙戊酸钠(VPA)联合全反式维甲酸(ATRA)对三维细胞培养模型中宫颈癌细胞的诱导分化作用。方法:将HeLa和SiHa细胞株接种于培养支架,构建宫颈癌细胞三维培养模型。实验分为VPA+ATRA组、VPA+ATRA+LY-294002组和对照组(加等量DMSO)。培养2周后,收获支架,石蜡包埋、切片。采用免疫荧光检测维甲酸受体β2(RARβ2)、E-cadherin及上皮细胞分化指标Filaggrin、Involucrin和Loricrin的表达;利用免疫荧光双标技术检测p-Akt/E-cadherin及p-Akt/Involucrin表达的时空关系,分析PI3K/Akt通路的调控作用。结果:与对照组比较,VPA联合ATRA上调三维培养模型中HeLa和SiHa细胞RARβ2及E-cadherin的表达(P

Objective: To investigate cervical cancer cells differentiation in three-dimensional culture model induced by the combination of valproic acid( VPA) and all-trans retinoic acid( ATRA).Methods: HeLa and SiHa cells were seeded and cultured in three-dimensional culture scaffolds. The cells were assigned into three groups based on the treatment received: VPA( 3mmol / L) and ATRA( 1mmol / L) in combination,VPA and ATRA plus PI3K inhibitor LY-294002,and vehicle only. After 2-week culture,the scaffolds were harvested,embedded in paraffin and sliced.Th...