Identification and characterization of two peptidoglycan recognition proteins with zinc-dependent antibacterial activity from the cotton bollworm, Helicoverpa armigera
We thank Dr. David Anstorm for revising the manuscript. This work was supported by Grants 31000982 and 31101672 from the National Natural Science Foundation of China .
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
Peptidoglycan recognition proteins (PGRPs) specifically bind to peptidoglycan and play an important role in the innate immune responses as pattern recognition receptors (PRRs). Here we identified and characterized two PGRPs (HaPGRP-B and HaPGRP-C) from the cotton bollworm, Helicoverpa armigera. The comparative analysis indicated that five amino acids which are required for T7 lysozyme Zn2+ binding and amidase activity are conserved in HaPGRP-B and HaPGRP-C, suggesting that the two PGRPs are members of the amidase-type PGRPs. HaPGRP-B and HaPGRP-C mRNA increased in both the fat bodies and the h...