The article is concerned with the moditicatton ot lipase witn Tween-80 or glutaraldehyde to connect Tween-80 and lipase covalently. Comparing the difference between modified lipase and nature lipase, we can get some conclusion. The results are follows: with the same concentration of formaldehyde and glutaraldehyde, the activity of lipase modified by formaldehyde is higher than lipase which was modified by glutaraldehyde; while the lipase modified by glutaraldehyde has higher connecting efficiency than lipase modified by formaldehyde. With the ...