期刊论文

中文

生态毒理学报

1673-5897

2011

6

1

110-112

国家科技部国际科技合作项目（No.2010DFA31790）；

本校为第一机构

为研究型谷胱甘肽(GSH)对甲醛所致Hela细胞毒性的影响,采用体外染毒方式,应用KCl-SDS法和MTT法分别检测GSH对甲醛引起Hela细胞DNA-蛋白质交联(DPC)和细胞活性的影响。结果表明,250μmol.L-1浓度下,DNA-蛋白质交联实验中甲醛染毒组所致的DPC显著高于对照组(p<0.01),GSH+甲醛组所致的DPC不但显著高于对照组(p<0.01),还显著高于甲醛染毒组(p<0.01),MTT实验中甲醛染毒组的细胞活性显著低于对照组(p<0.01),GSH+甲醛组所致的细胞活性不但显著低于对照组(p<0.01),还显著低于甲醛染毒组(p<0.01)。上述结果表明,GSH单独作用对Hela细胞无明显毒性作用,但是对甲醛所致毒性作用有显著促进...

The influence of glutathione（GSH） on toxicity of Hela cells induced by formaldehyde was studied.Hela cells were exposed to 250 μmol·L-1 formaldehyde,glutathione,the mixture of GSH and FA and a control,then KCl-SDS and MTT methods were used to determine DNA-protein crosslinks（DPC） and the activity of cells respectively.The results show that 250 μmol·L-1 formaldehyde induced significant DPC formation and decreased cell activity compared with the control group（p0.01）.At the same time,the mixture group could also induce significant DPC fo...