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MOLECULAR CHARACTERIZATION OF AUTOPHAGY-RELATED GENE 5 FROM Spodoptera exigua AND EXPRESSION ANALYSIS UNDER VARIOUS STRESS CONDITIONS

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成果类型:
期刊论文
作者:
Liu, Kai-Yu;Xia, Yu-Qian;Zhou, Jing;Chen, Zu-Wen;Lu, Dandan;...
通讯作者:
Ai, Hui(刘绪生
作者机构:
[Liu, Xu-Sheng; Zhou, Jing; Zhang, Ning-Zhao; Liu, Kai-Yu; Ai, Hui; Xia, Yu-Qian; Chen, Zu-Wen; Lu, Dandan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
[Zhou, Li-Lin] Wuhan Vegetable Res Inst, Dept Plant Protect, Wuhan, Peoples R China.
通讯机构:
[Ai, Hui] C
Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
语种:
英文
关键词:
Spodoptera exigua;ATG5;autophagy;qPCR;Western blot
期刊:
Archives of Insect Biochemistry and Physiology
ISSN:
0739-4462
年:
2016
卷:
92
期:
4
页码:
225-241
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31472052, 31172162, 31372260]
机构署名:
本校为第一且通讯机构
院系归属:
生命科学学院
摘要:
Autophagy is not only involved in development, but also has been proved to attend immune response against invading pathogens. Autophagy protein 5 (ATG5) is an important autophagic protein, which plays a crucial role in autophagosome elongation. Although ATG5 has been well studied in mammal, yeast, and Drosophila, little is known about ATG5 in lepidopteran insects. We cloned putative SeAtg5 gene from Spodoptera exigua larvae by the rapid amplification of cDNA ends method, and its characteristics and the influences of multiple exogenous factors on its expression levels were then investigated. Th...

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