期刊论文

中文

环境科学学报

0253-2468

2006

26

3

501-504

10.3321/j.issn:0253-2468.2006.03.024

国家自然基金项目（NO.30570799）~~；

本校为第一机构

为了探讨气态甲醛致生物体内DNA-DNA交联效应,进一步评价甲醛的遗传毒性作用,以昆明纯系小鼠为实验材料,进行了72 h动态吸入式连续染毒,采用荧光检测法检测甲醛染毒后小鼠肝细胞DNA-DNA交联形成的效应.实验结果表明,0.5 mg·m^-3的气态甲醛能引起明显的DNA-DNA交联（p＜0.05）,较高浓度的甲醛（1.0mg·m^-3、3.0 mg·m^-3,p＜0.01）可以产生极为明显的DNA-DNA交联作用.以上实验结果显示了0.5mg·m^-3的气态甲醛就能致生物体内DNA-DNA交联效应,且随着浓度的升高DNA-DNA交联率越高.

In order to explore the effect of DNA-DNA crosslinks and estimate the genotoxicity induced by formaldehyde the experiment objects, which were exposed to the air formaldehyde in 72 hours. This experiment applied fluorescence this study used Kunming mice as t to detect DNA-DNA crosslinks formation effect of the liver cells of the mice after the exposure to air formaldehyde. Results show that 0.5 mg·m^-3 air formaldehyde can induce DNA-DNA crosslinks formation in vivo （p 〈0.05）, and in a dose-dependent manner at higher concentrations （ 1.0 mg...