期刊论文

Betzig, Eric

英文

Science

0036-8075

2015

349

6251

aab3500

10.1126/science.aab3500

Howard Hughes Medical Institute (HHMI)Howard Hughes Medical Institute; National Basic Research Program (973 Program) of ChinaNational Basic Research Program of China [2013CB910103]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31370851]; Beijing Natural Science Foundation, ChinaBeijing Natural Science Foundation [7131011]; NIHUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [GM-075252]; NATIONAL HEART, LUNG, AND BLOOD INSTITUTEUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Heart Lung & Blood Institute (NHLBI) [ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514, ZIAHL000514] Funding Source: NIH RePORTER; NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCESUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute of General Medical Sciences (NIGMS) [R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252, R01GM075252] Funding Source: NIH RePORTER

本校为其他机构

Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and nonlinear SIM with patterned activation at 45- to 62...