期刊论文

中文

植物保护学报

0577-7518

2011

38

4

333-338

10.13802/j.cnki.zwbhxb.2011.04.003

国家自然科学基金（30870350）；

本校为第一机构

为明确vasa基因在中华卵索线虫Ovomermis sinensis生殖系发育以及原始生殖细胞增殖过程中的作用,采用同源克隆与cDNA末端快速扩增技术（RACE）,从中华卵索线虫中克隆得到vasa基因全长cDNA序列（3053bp）,编码813个氨基酸,具有DEAD-box家族蛋白全部9个保守基序。经同源比对与系统进化树分析,确定其属于DEAD-box家族的VASA亚家族成员。利用反转录PCR（reverse transcription PCR,RT-PCR）以及实时荧光定量PCR（real time PCR）分析,vasamRNA在中华卵索线虫性腺中专一性表达,其它部位如头、尾、体壁均未检测到vasa表达信号,且在生殖腺发育初期表达量最高。此外,vasa基因转录本在中华卵索线虫胚胎发...

In order to understand the function of vasa gene in the development of germ line and the reproduction of primordial germ cells in Ovomermis sinensis, the full-length cDNA sequence of Os-vasa was cloned from the mermithid by rapid amplification of cDNA end （RACE） methods. The full length of Osvasa cDNA is 3 053 bp, encoding 813 amino acids. The putative amino acid sequence possesses all nine conserved motifs of the DEAD-box family. High identity of VASA in amino acid sequence （more than 58% ） was observed between O. sinensis and the other or...