期刊论文

中文

昆虫学报

0454-6296

2006

49

3

363-366

10.3321/j.issn:0454-6296.2006.03.002

30470246:中国科学院项目（非规范项目） 2005ABA130:湖北省自然科学基金

本校为第一机构

利用RT-PCR和RACE方法,获得了棉铃虫Helicoverpa armigera酚氧化酶原（prophenoloxidase,PPO）基因一个亚型cDNA的完整序列.该序列全长2 405 bp,含有一个2 097 bp的开放阅读框,编码一个由698个氨基酸残基组成的蛋白质.推导的氨基酸序列与其他鳞翅目昆虫PPO2基因相应氨基酸序列有较高的同源性（76%～80%）,同时该序列具有铜离子结合位点等PPO基因所具有的典型特征.组织特异性表达分析表明,该基因在棉铃虫血细胞、体壁和中肠中均有表达.

The cDNA of Helicoverpa armigera prophenoloxidase （PPO） was cloned by means of RT-PCR and RACE. The cDNA was 2 405 bp in length and contained an open reading frame （ORF） of 2 097 bp which encoded 698 amino acid residues. The deduced amino acid sequence showed a high identity to the reported sequence of PPO2 from other insects and shared the typical structural features of PPO from other insects. The results of RT-PCR showed that PPO mRNA was expr...