作者机构：
[李中强; 汪正祥; 谭庆; 余曼] College of Resources and Environment, Hubei University, Wuhan 430062, China;[雷耘] College of Life Science, Central China Normal University, Wuhan 430079, China
通讯机构：
[Tan, Q.] C;College of Resources and Environment, Hubei University, China
作者机构：
College of Life Science, Central China Normal University, Wuhan 430079, China;College of Life Science, Central China Normal University, Wuhan 430079, China Key Laboratory of Pest;Key Laboratory of Pesticide & Chemical Biology (CCNU) of Ministry of Education, Wuhan 430079, China
会议名称：
The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)
会议时间：
2008-05-16
会议地点：
上海
会议论文集名称：
The 2nd International Conference on Bioinformatics and Biomedical Engineering(iCBBE 2008)(第二届生物信息与生物医学工程国际会议)论文集
摘要：
Streptococcus pneumoniae is the major causative organisms of meningitis, bacteremia, and pneumonia. The drug resistance of S.pneumoniae to antibiotics showed ascending tendency. The present study cloned the full-length HMG-CoA reductase (HMGR) gene of S.pneumoniae and expressed in E.coli BL21 (DE3). The His-tag containing recombinant protein was purified by Ni-NTA affinity chromatography. Statins represent a kind of excellent competing inhibitor targeted for the class Ι HMG-CoA reductase, but were relatively poor to the class II enzyme of important bacterial pathogens. In order to develop new inhibitors with more specific and stronger function targeted for the class II HMG-CoA reductase, the 3D model of S.pneumoniae HMG-CoAR was built based on structure template of HMGR of Pseudomonas mevalonii by homology modeling using composer module of SYBYL7.0 program.
通讯机构：
[Li, Xue-Bao] H;Hua Zhong Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), are usually involved in cell development in plants. To investigate the expression profiling as well as the role of FLA genes in fiber development, 19 GhFLA genes (cDNAs) were isolated from cotton (Gossypium hirsutum). Among them, 15 are predicted to be glycosylphosphatidylinositol anchored to the plasma membranes. The isolated cotton FLAs could be divided into four groups. Real-time quantitative reverse transcriptase polymerase chain reaction results indicated that the GhFLA genes are differentially expressed in cotton tissues. Three genes (GhFLA1/2/4) were specifically or predominantly expressed in 10 days post-anthesis fibers, and the transcripts of the other four genes (GhFLA6/14/15/18) were accumulated at relatively high levels in cotton fibers. Furthermore, expressions of the GhFLA genes are regulated in fiber development and in response to phytohormones and NaCl. The identification of cotton FLAs will facilitate the study of their roles in cotton fiber development and cell wall biogenesis.
作者机构：
College of Life Science, Central China Normal University, Wuhan 430079, China;Nanyang Technological;Nanyang Technological University, Nanyang Avenue, 639798, Singapore
会议名称：
第十届室内空气品质和质量国际学术会议(Proceedings of the 10th International Conference on Indoor Air Quality and Climate)
摘要：
Purpose: Whether formaldehyde induces DNA breakage or not is still in debate. So far it is not clear whether dose-effect between formaldehyde and DNA breakage takes place while the DPC exists. Approach: In this study we had chosen human buccal cells as materials to explore the genotoxicity of liquid and gaseous formaldehyde with comet assay. Findings: The results showed that liquid formaldehyde induced DNA breakage in the range from 5 μM to 10 μM (P＜0.05, compared with control group), and 7.5 μM was found to induce DNA breakage most. The DNA crosslinks were significantly found at 15 μM, 30 μM and 50 μM (P＜0.01, compared with 7.5 μM group).DNA breakage caused by gaseous formaldehyde was found at 0.5 mg/m3 and 1.0 mg/m3 (P＜0.01, compared with control group) but DNA crosslinks at 3.0 mg/m3 significantly. Conclusions: The results suggest that formaldehyde induces DNA breakage in a very low concentration range but induces DNA crosslinks in a higher range.
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY,2008年148(4):459-459 ISSN：1532-0456
作者机构：
School of Life Sciences, Huazhong Normal University, Wuhan 430079, China;School of Life Sciences, East China Normal University, Shanghai, 200062, China
