作者机构:
[Liao, Cai-Yi; Liu, Wen-Cheng; Song, Ru-Feng; Chen, Hui-Hui; Zhang, Yu; Guo, Jia-Xing; Lu, Kai-Kai; Zuo, Jia-Xin; Hu, Xiao-Yu] Henan Univ, Coll Life Sci, Collaborat Innovat Ctr Crop Stress Biol, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Peoples R China.;[Ren, Feng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Lu, Ying-Tang] Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Peoples R China.
通讯机构:
[Wen-Cheng Liu] S;State Key Laboratory of Crop Stress Adaptation and Improvement, Collaborative Innovation Center of Crop Stress Biology, College of Life Sciences, Henan University , Kaifeng 475004 , China
摘要:
SALT OVERLY SENSITIVE1 (SOS1) is a key component of plant salt tolerance. However, how SOS1 transcription is dynamically regulated in plant response to different salinity conditions remains elusive. Here, we report that C-type Cyclin1;1 (CycC1;1) negatively regulates salt tolerance by interfering with WRKY75-mediated transcriptional activation of SOS1 in Arabidopsis (Arabidopsis thaliana). Disruption of CycC1;1 promotes SOS1 expression and salt tolerance in Arabidopsis because CycC1;1 interferes with RNA polymerase II recruitment by occupying the SOS1 promoter. Enhanced salt tolerance of the cycc1;1 mutant was completely compromised by an SOS1 mutation. Moreover, CycC1;1 physically interacts with the transcription factor WRKY75, which can bind to the SOS1 promoter and activate SOS1 expression. In contrast to the cycc1;1 mutant, the wrky75 mutant has attenuated SOS1 expression and salt tolerance, whereas overexpression of SOS1 rescues the salt sensitivity of wrky75. Intriguingly, CycC1;1 inhibits WRKY75-mediated transcriptional activation of SOS1 via their interaction. Thus, increased SOS1 expression and salt tolerance in cycc1;1 were abolished by WRKY75 mutation. Our findings demonstrate that CycC1;1 forms a complex with WRKY75 to inactivate SOS1 transcription under low salinity conditions. By contrast, under high salinity conditions, SOS1 transcription and plant salt tolerance are activated at least partially by increased WRKY75 expression but decreased CycC1;1 expression. CycC1;1 forms a transcriptional repression complex with the transcription factor WRKY75 to downregulate SOS1 expression, thereby negatively regulating salt stress tolerance in Arabidopsis.
摘要:
Abstract: Genome streamlining, as a natural process in the evolution of microbes, has become a common approach for generating ideal chassis cells for synthetic biology studies and industrial applications. However, systematic genome reduction remains a bottleneck in the generation of such chassis cells with cyanobacteria, due to very time-consuming genetic manipulations. Synechococcus elongatus PCC 7942, a unicellular cyanobacterium, is a candidate for systematic genome reduction, as its essential and nonessential genes have been experimentally identified. Here, we report that at least 20 of the 23 over 10 kb nonessential gene regions could be deleted and that stepwise deletions of these regions could be achieved. A septuple-deletion mutant (genome reduced by 3.8%) was generated, and the effects of genome reduction on the growth and genome-wide transcription were investigated. In the ancestral triple to sextuple mutants (b, c, d, e1), an increasingly large number of genes (up to 998) were upregulated relative to the wild type, while slightly fewer genes (831) were upregulated in the septuple mutant (f). In a different sextuple mutant (e2) derived from the quintuple mutant d, much fewer genes (232) were upregulated. Under the standard conditions in this study, the mutant e2 showed a higher growth rate than the wild type, e1 and f. Our results indicate that it is feasible to extensively reduce the genomes of cyanobacteria for generation of chassis cells and for experimental evolutionary studies. Keywords: genome reduction; nonessential gene regions; transcriptomic profiles; growth conditions; CRISPR/Cpf1
通讯机构:
[Haobin Zhao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
摘要:
Abstract: Protein arginine methyltransferase 5 (Prmt5), conserved from yeast to humans, catalyzes arginine’s dimethylation in proteins. Prmt5 is necessary for embryonic development in mice because it maintains embryonic stem cells. However, the embryos of zebrafish (Danio rerio) remain viable with a deficiency in germ cells and sexual development after the knockout of prmt5. Therefore, it was considered whether prmt5 is dispensable during embryogenesis in fish. Medaka (Oryzias latipes), another model fish organism, was used in this experiment. The medaka prmt5 was mutated with Transcription Activator-Like Effector Nucleases (TALEN) causing the premature stopping of transcription. None of the homozygous prmt5 mutant fish were viable, only the heterozygous offspring survived. Quantitative reverse transcription-polymerase chain reaction (qPCR) results showed a significant decrease in octamer-binding transcription factor 4 (oct4), homeobox transcription factor nanog (nanog), vasa, B-cell CLL/lymphoma 2 (bcl2), and the ratio of bcl2 to bax (bcl2 associated x), and a significant increase in caspase3 and caspase8 in the embryos of the heterozygous prmt5 mutant compared with that of the wild type. The results showed that the mutation of prmt5 caused down-regulation of the genes functioning in stemness and up-regulation of the genes in the cascade of cell death. These results suggested that prmt5 is necessary for embryogenesis via maintaining stemness and repressing apoptosis in medaka. Keywords: medaka; prmt5; gene editing; embryonic development; gene expression; stemness; apoptosis
通讯作者:
Cuihong Wan<&wdkj&>Cuihong Wan Cuihong Wan Cuihong Wan
作者机构:
[Wan, Cuihong; Huwanixi, Aishuake; Peng, Zhao] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Wan, Cuihong; Huwanixi, Aishuake; Peng, Zhao] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.;[Wan, Cuihong] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Wan, Cuihong] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构:
[Cuihong Wan; Cuihong Wan Cuihong Wan Cuihong Wan] S;School of Life Sciences and Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Wuhan, Hubei, PR China
摘要:
Nostoc flagelliforme, a terrestrial cyanobacterium spread throughout arid and semi-arid areas, has been long known for its outstanding adaptability to extremely dry conditions. This microorganism is able to recover biological activities within hours after months of anhydrobiosis state, attracting investigation through proteomic analysis. Except for canonical proteome, microproteins encoded by small ORFs (smORFs) have recently been regarded as indispensable participants in metabolic processes. However, the involvement of smORFs in N. flagelliforme remains unknown. Here we first constructed a smORF database in N. flagelliforme using bioinformatic prediction, resulting in 6072 novel smORFs. Then LS-MS/MS analysis was applied to identify expression patterns of microproteins and seek smORFs and their encoded microprotein playing a role during rehydration. In total, 18 novel microproteins were mined based on a smORF searching strategy combined with three proteomic assays, of which five were annotated as ribosomal proteins, one as RNA polymerase subunit, and one as acetohydroxy acid isomeroreductase. We also suggested the possible functions of smORFs according to their expression pattern and discovered two neighboring and homologous smORFs. All these results will expand our knowledge of smORFs-encoded microproteins and their relation to the stress response of extremophilic microorganisms.
作者:
Luo, Jinhong*;Lu, Manman;Luo, Jie;Moss, Cynthia F.
期刊:
Journal of Comparative Physiology A,2023年209(1):203-214 ISSN:0340-7594
通讯作者:
Luo, Jinhong;Moss, Cynthia F
作者机构:
[Lu, Manman; Luo, Jie; Luo, Jinhong] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.;[Moss, Cynthia F.] Johns Hopkins Univ, Dept Psychol & Brain Sci, Baltimore, MD 21218 USA.
通讯机构:
[Luo, Jinhong] I;[Moss, Cynthia F] D;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China.;Department of Psychological and Brain Sciences, Johns Hopkins University, Baltimore, MD, 21218, USA. cynthia.
摘要:
Diverse animal taxa are capable of rapidly modifying vocalizations to mitigate interference from environmental noise. Echolocating bats, for example, must frequently perform sonar tasks in the presence of interfering sounds. Numerous studies have documented sound production flexibility in echolocating bats; however, it remains unknown whether noise-induced vocal modifications (NIVMs) mitigate interference effects on echoes or calls. In this study, we leverage echo level compensation behavior of echolocating bats to answer this question. Using a microphone array, we recorded echolocation calls of Hipposideros pratti trained to approach and land on a perch in the laboratory under quiet and noise conditions. We found that H. pratti exhibited echo level compensation behavior during approaching flights, which depended critically on distance to the landing perch. Broadcast noise delayed and affected the rate of echo level compensation in H. pratti. Moreover, H. pratti increased vocalization amplitude, i.e., exhibited the Lombard effect, while also adjusting call duration and bandwidth with increasing noise levels. Quantitative analyses of the data show that H. pratti relies on echo feedback, not vocal feedback, to adjust signals in the presence of noise. These findings provide compelling evidence that NIVMs in echolocating animals and non-echolocating animals operate through different mechanisms.
期刊:
Annals of Botany,2023年132(6):viii-x ISSN:0305-7364
通讯作者:
Huang, SQ
作者机构:
[Huang, Shuang-Quan; Huang, SQ] Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
通讯机构:
[Huang, SQ ] C;Cent China Normal Univ, Sch Life Sci, Inst Evolut & Ecol, Wuhan 430079, Peoples R China.
关键词:
Enantiostyly;floral sexual polymorphism;geitonogamous self-pollination;tracing pollen movement
摘要:
This article comments on:Steven D. Johnson, Jeremy J. Midgley and Nicola Illing. The enantiostylous floral polymorphism of Barberetta aurea (Haemodoraceae) faci
摘要:
The Lombard effect, ref erring to an involuntary rise in vocal intensity, is a widespread vertebrate mecha-nism that aims to maintain signal efficiency in response to ambient noise. Previous studies showed that the Lombard effect could be sufficiently implemented at subcortical levels and operated by continuously monitoring background noise, requiring some subcortical auditory sensitive neurons to have continuous responses to background noise. However, such neurons have not been well characterized. The inferior colliculus (IC) is a major auditory integration center under the auditory cortex and provides projections to the putative vocal pattern generator in the brainstem. Thus, it is reasonable to speculate that the IC is a likely auditory nucleus candidate having background noise responding neurons (BNR neurons). In the present study, we isolated 183 sound-sensitive IC neurons in a constant frequency-frequency mod-ulation bat, Hipposideros pratti, and found that around 19% of these IC neurons are BNR neurons when stimulated with 70 dB SPL background white noise. Their firing rates in response to noise increased with increasing noise intensity and could be suppressed by sound stimulation. Furthermore, compared to neu-rons with similar best frequencies, the BNR neurons had smaller Q 10-dB values and lower noise-induced minimal threshold change, indicating that BNR neurons received fewer inhibitory inputs. These results suggested that the BNR neurons are ideal candidates for collecting information about background noise. We proposed that the BNR neurons synapsed with neurons in vocal-pattern-generating networks in the brainstem and initiated the Lombard effect by a feed-forward loop. (c) 2023 Elsevier B.V. All rights reserved.
作者机构:
[Yan Peng; Yutao Xiao; Qi Xu; Peng Cui; Minghui Jin; Lei Zhang; Bo Liu; Hangwei Liu; Yongfeng Zhou; Bo Yang; Weigang Zheng; Kenneth Wilson] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518116, China;[Yan Zhou; Minghui Jin; Kongming Wu] The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing 100193, China;[Chris Jiggins; Henry L.North] Department of Zoology, University of Cambridge, Cambridge CB2 1SZ, UK;[Yan Peng] College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China;[Ruiqing Pan] Berry Genomics Corporation, Beijing 102200, China
摘要:
The cotton bollworm, Helicoverpa armigera, is set to become the most economically devastating crop pest in the world, threatening food security and biosafety as its range expands across the globe. Key to understanding the eco-evolutionary dynamics of H.armigera, and thus its management, is an understanding of population connectivity and the adaptations that allow the pest to establish in unique environments. We assembled a chromosome-scale reference genome and re-sequenced 503 individuals spanning the species range to delineate global patterns of connectivity, uncovering a previously cryptic population structure. Using a genome-wide association study (GWAS) and cell line expression of major effect loci, we show that adaptive changes in a temperature- and light-sensitive developmental pathway enable facultative diapause and that adaptation of trehalose synthesis and transport underlies cold tolerance in extreme environments. Incorporating extensive pesticide resistance monitoring, we also characterize a suite of novel pesticide and Bt resistance alleles under selection in East China. These findings offer avenues for more effective management strategies and provide insight into how insects adapt to variable climatic conditions and newly colonized environments.
期刊:
American Journal of Botany,2023年110(1) ISSN:0002-9122
作者机构:
[Huang, Shuang-Quan; Xu, Qi; Hao, Kai] Cent China Normal Univ, Inst Evolut & Ecol, Sch Life Sci, Wuhan 430079, Peoples R China.;[Hao, Kai] Chinese Acad Sci, Key Lab Plant Resources Conservat & Sustainable Ut, South China Bot Garden, Guangzhou 510650, Peoples R China.
关键词:
Geranium;chemical defense;generalist insects;pollen feeding;pollen grain size;pollen nutrition;pollen transfer efficiency
摘要:
PREMISE: Why have pollen grains evolved to be exceptionally large in some species? Pollen feeding hypothesis suggests that if the proportion of pollen amounts for feeding is reduced in a flower, the low allocation to pollen number would allow pollen grains to be larger. METHODS: To examine whether species with large pollen grains experience low pollen consumption, the behavior of insects feeding on nectar and pollen was observed and pollen transfer efficiency was estimated for four visitor types in Geranium delavayi. To see whether bees actively collected pollen, the numbers of grains in pollen baskets and on the body were compared. Both nutritional value (total protein and lipid) and chemical defense (phenolic metabolites) in pollen against pollen feeders were measured. RESULTS: Bumblebees and honeybees foraged for nectar, rarely groomed pollen into corbiculae, and had > 5× higher pollen transfer efficiency than smaller solitary bees and flies, which were pollen eaters that removed more pollen but deposited less. Pollen grains were characterized by low protein and high lipid content with a low protein-lipid ratio, an unfavorable combination for bumblebees. Three secondary metabolites were significantly higher in pollen grains (7.77 mg/g) than in petals (1.08 mg/g) and in nectar (0.44 mg/g), suggesting stronger chemical defense in pollen. CONCLUSIONS: Our results indicated that large bees took nectar but little of the nutritionally poor and highly toxic pollen. These data support one prediction of the pollen feeding hypothesis, that species with few and large pollen grains would also have low pollen consumption rates. This article is protected by copyright. All rights reserved.
期刊:
Journal of Translational Medicine,2023年21(1):1-18 ISSN:1479-5876
通讯作者:
Zhenbo Song<&wdkj&>Lei Liu
作者机构:
[Sun, Ying; Li, Jingyi; Wang, Guannan; Huang, Yanxin; Zheng, Lihua; Song, Zhenbo; Yang, Qifang; Liu, Lei; Zhang, Ming] Northeast Normal Univ, Natl Engn Lab Druggable Gene & Prot Screening, Changchun, Peoples R China.;[Li, Aoqiang; Zhang, Ming] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Peoples R China.;[Zhang, Muqing] Univ Illinois, Sch Mol & Cellular Biol, Urbana, IL USA.
通讯机构:
[Zhenbo Song; Lei Liu] N;National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China<&wdkj&>National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun, China
摘要:
The realization of the “microbiota–gut–brain” axis plays a critical role in neuropsychiatric disorders, particularly depression, is advancing rapidly. Matrine is a natural bioactive compound, which has been found to possess potential antidepressant effect. However, the underlying mechanisms of regulation of the “microbiota–gut–brain” axis in the treatment of depression by oral matrine remain elusive. Its antidepressant effects were initially evaluated by behavioral tests and relative levels of monoamine neurotransmitters, and matrine has been observed to attenuate the depression-like behavior and increase neurotransmitter content in CUMS-induced mice. Subsequently, studies from the “gut” to “brain” were conducted, including detection of the composition of gut microbiota by 16S rRNA sequencing; the metabolomics detection of gut metabolites and the analysis of differential metabolic pathways; the assessment of relative levels of diamine oxidase, lipopolysaccharide, pro-inflammatory cytokines, and brain-derived neurotrophic factor (BDNF) by ELISA kits or immunofluorescence. Matrine could regulate the disturbance of gut microbiota and metabolites, restore intestinal permeability, and reduce intestinal inflammation, thereby reducing the levels of pro-inflammatory cytokines in peripheral blood circulation and brain regions, and ultimately increase the levels of BDNF in brain. Matrine may ameliorate CUMS-induced depression in mice by modulating the “microbiota–gut–brain” axis.
作者机构:
[Yang, Yi-Wen; Chen, Si-Zhuo; Huang, Da; Qiu, Bao-Sheng; Yu, Chen; Liu, Ke; Qiu, BS] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yang, Yi-Wen; Chen, Si-Zhuo; Huang, Da; Qiu, Bao-Sheng; Yu, Chen; Liu, Ke; Qiu, BS] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Yi-Wen] Jiujiang Univ, Coll Pharm & Life Sci, Jiujiang 332000, Jiangxi, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
通讯机构:
[Chen, M ] U;[Qiu, BS ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
摘要:
Expanded orange carotenoid protein paralogs in subaerial Nostoc species functionally specialize as singlet oxygen quenchers or phycobilisome fluorescence quenchers to survive desiccation tolerance. Orange carotenoid protein (OCP) is a photoactive protein that participates in the photoprotection of cyanobacteria. There are 2 full-length OCP proteins, 4 N-terminal paralogs (helical carotenoid protein [HCP]), and 1 C-terminal domain-like carotenoid protein (CCP) found in Nostoc flagelliforme, a desert cyanobacterium. All HCPs (HCP1 to 3 and HCP6) from N. flagelliforme demonstrated their excellent singlet oxygen quenching activities, in which HCP2 was the strongest singlet oxygen quencher compared with others. Two OCPs, OCPx1 and OCPx2, were not involved in singlet oxygen scavenging; instead, they functioned as phycobilisome fluorescence quenchers. The fast-acting OCPx1 showed more effective photoactivation and stronger phycobilisome fluorescence quenching compared with OCPx2, which behaved differently from all reported OCP paralogs. The resolved crystal structure and mutant analysis revealed that Trp111 and Met125 play essential roles in OCPx2, which is dominant and long acting. The resolved crystal structure of OCPx2 is maintained in a monomer state and showed more flexible regulation in energy quenching activities compared with the packed oligomer of OCPx1. The recombinant apo-CCP obtained the carotenoid pigment from holo-HCPs and holo-OCPx1 of N. flagelliforme. No such carotenoid transferring processes were observed between apo-CCP and holo-OCPx2. The close phylogenetic relationship of OCP paralogs from subaerial Nostoc species indicates an adaptive evolution toward development of photoprotection: protecting cellular metabolism against singlet oxygen damage using HCPs and against excess energy captured by active phycobilisomes using 2 different working modes of OCPx.
摘要:
Engleromyces goetzei Henn (EgH) is a natural fungus that has been used as a traditional edible and medicine for long time in Southwest China. Our study found EgH aqueous extract (EgH‐AE) has very strong activities on antioxidant and anti‐inflammation. At the same time, we also found EgH‐AE has good biocompatibility and cell protective function, so it is biosafe, EgH‐AE may have the prospect of developing into functional beverage. Abstract High body mass index (high BMI, obesity) is a serious public health problem, and “obesity‐induced oxidative stress, inflammation, and cancer” have become modern epidemic diseases. We carried out this study to explore a functional beverage that may protect against obesity‐induced diseases. The Engleromyces goetzei Henn herbal tea is such a candidate. For this study, we carried out LC–MS analysis of E. goetzei Henn aqueous extract (EgH‐AE); then used the Caco‐2 cell line for the model cells and treated the cells with t‐BHP to form an oxidative stress system. An MTT assay was used for testing the biocompatibility and cytoprotective effects; reactive oxygen species and malondialdehyde determination was used for evaluating the antioxidative stress effect; TNF‐α and IL‐1β were used for observing the anti‐inflammatory effect, and 8‐OHdG for monitoring anticancer activity. The results of this study demonstrate that the EgH‐AE has very good biocompatibility with the Caco‐2 cell line and has good cytoprotective, antioxidant, anti‐inflammatory, and anticancer properties. It is clear that EgH‐AE, a kind of ancient herbal tea, may be used to develop a functional beverage that can be given to people with a high BMI to protect against obesity‐induced diseases.
期刊:
Journal of Environmental Chemical Engineering,2023年11(3):109717 ISSN:2213-3437
通讯作者:
Liao, XM;Zhang, Lizhi;Sun, HW;Zhang, LZ
作者机构:
[Liao, Xiaomei; Liu, Qianhui; Chen, Yangyang; Mei, Ting; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, Lizhi; Sun, Hongwei] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;[Zhang, Lizhi] Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China.;[Liao, XM] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, LZ; Sun, Hongwei] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
通讯机构:
[Liao, XM ; Zhang, LZ; Sun, HW ; Zhang, LZ ] ;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China.
关键词:
Sewage sludge;Ozonation;Zero-valent iron;Antibiotic resistance genes;Reactive oxygen species
摘要:
Wastewater treatment plants (WWTPs) are hotspots of antibiotic resistance genes (ARGs), and efficient technologies are needed to prevent the enrichment and spread of ARGs in municipal sludge. Herein, we demonstrated that oxalated zero-valent iron (OA-ZVIbm) catalyzed ozonation could effectively remove typical ARGs, tetG and sul1, from municipal sewage sludge, with the total abundance reduced by 3.02 and 2.00 log per gram sludge, respectively, surpassing the O-3 alone and O-3/ZVI(bm) counterparts. Meanwhile, the O-3/OA-ZVI(bm) treatment could simultaneously remove both intracellular and extracellular ARGs from the sludge, moreover, it also eliminated 1.83 log total Class 1 integron (total intI1), thus may suppress the horizontal gene transfer (HGT) potential of ARGs. The good performance of O-3/OA-ZVI(bm) was owing to the FeC2O4 center dot 2H(2)O shell of ZVI, which improved the accessibility of core iron species to catalyze the decomposition of O-3, producing more reactive oxygen species (ROSs) including center dot OH, center dot O-2(-), and O-1(2), for the removal of ARGs. This study provides a new method to control the dissemination of ARGs in sewage sludge.
通讯机构:
[Qiu, BS ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要:
Hypothetical chloroplast open reading frames (ycfs) are putative genes in the plastid genomes of photosynthetic eukaryotes. Many ycfs are also conserved in the genomes of cyanobacteria, the presumptive ancestors of present-day chloroplasts. The functions of many ycfs are still unknown. Here, we generated knock-out mutants for ycf51 (sll1702) in the cyanobacterium Synechocystis sp. PCC 6803. The mutants showed reduced photoautotrophic growth due to impaired electron transport between photosystem II (PSII) and PSI. This phenotype results from greatly reduced PSI content in the ycf51 mutant. The ycf51 disruption had little effect on the transcription of genes encoding photosynthetic complex components and the stabilization of the PSI complex. In vitro and in vivo analyses demonstrated that Ycf51 cooperates with PSI assembly factor Ycf3 to mediate PSI assembly. Furthermore, Ycf51 interacts with the PSI subunit PsaC. Together with its specific localization in the thylakoid membrane and the stromal exposure of its hydrophilic region, our data suggest that Ycf51 is involved in PSI complex assembly. Ycf51 is conserved in all sequenced cyanobacteria, including the earliest branching cyanobacteria of the Gloeobacter genus, and is also present in the plastid genomes of glaucophytes. However, Ycf51 has been lost from other photosynthetic eukaryotic lineages. Thus, Ycf51 is a PSI assembly factor that has been functionally replaced during the evolution of oxygenic photosynthetic eukaryotes. Chloroplast reading frame Ycf51 is a photosystem I assembly factor conserved in all sequenced cyanobacteria and glaucophyte plastids but lost from other photosynthetic eukaryotic lineages.
摘要:
Algicidal bacteria can inhibit the growth of algae or lyse algal cells, thus playing roles in shaping aquatic microbial communities and maintaining the functions of aquatic ecosystems. Nevertheless, our understanding of their diversities and distributions remains limited. In this study, we collected water samples from 17 freshwater sites in 14 cities in China and screened a total of 77 algicidal bacterial strains using several prokaryotic cyanobacteria and eukaryotic algae as target strains. According to their target-specificities, these strains were classified into three subgroups, cyanobacterial algicidal bacteria, algal algicidal bacteria, and broad-target algicidal bacteria, each displaying distinctive compositions and geographical distribution patterns. They are assigned to Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes bacterial phyla, of which Pseudomonas and Bacillus are the most abundant gram-negative and gram-positive genus, respectively. A number of bacterial strains, such as Inhella inkyongensis and Massilia eburnean, are suggested as new algicidal bacteria. The diverse taxonomies, algal-inhibiting abilities and distributions of these isolates have suggested that there are rich algicidal bacterial resources in these aquatic environments. Our results provide new microbial resources for algal-bacterial interaction studies, and shed new insights into how algicidal bacteria can be used in the control of harmful algal blooms, as well as in algal biotechnology.
通讯机构:
[Xinmin Lu] S;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University , Wuhan 430070 , China<&wdkj&>Institute of Invasion Biology and Agriculture Ecological Safety, Huazhong Agricultural University , Wuhan 430070 , China<&wdkj&>College of Plant Sciences and Technology, Huazhong Agricultural University , Wuhan 430070 , China
摘要:
Soil biota, as legacy effects of previous species in natural ecosystems, profoundly affects plant performance in new habitats and, in turn, plant community. However, how soil biota, as legacy effects of agricultural crops, affects the likelihood of establishment of exotic and native plants in newly abandoned farmland remains poorly understood, which may hinder effective management of agricultural weeds. Here, we grew 58 plant species (28 exotic species and 30 native species) common in Central China in sterilized vs. nonsterilized soils collected from a newly abandoned maize field. We (i) estimated the effects of soil biota on plant shoot, root and total mass, (ii) explored the dependence of soil effects on the plants' phylogenetic distance to maize, origin (native vs. exotic) and life history (annual vs. perennial) and (iii) tested which plant traits could predict soil effects. Soil biota, in general, decreased plant mass, suggesting a dominant role of enemies. The effect of the soil biota on plant total mass was unrelated to the phylogenetic distance of the plants to maize and decreased linearly with increasing plant intrinsic growth ability. Moreover, the soil biota on average had greater negative impacts on the total mass of exotic plants, particularly perennial species, than on that of native plants. Our results suggest that plant intrinsic growth ability, rather than phylogenetic relatedness, is a reliable predictor of soil effects. Additionally, native plants can benefit from plant-soil interactions in competing with exotic perennial plants when initially established in newly abandoned farmlands.