期刊：
Computational and Structural Biotechnology Journal,2023年21:168-175 ISSN：2001-0370
通讯作者：
Xiongfeng Dai<&wdkj&>Manlu Zhu
作者机构：
[Han, Fei; Dai, Xiongfeng; Wang, Yanling; Zhu, Manlu; Wang, Qian; Mu, Haoyan] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan, Hubei, Peoples R China.
通讯机构：
[Xiongfeng Dai; Manlu Zhu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China<&wdkj&>Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei Province, China
关键词：
(p)ppGpp;Growth control;Resource allocation;Bacteria;Plant and green algaes
摘要：
Rapid growth and survival are two key traits that enable bacterial cells to thrive in their natural habitat. The guanosine tetraphosphate and pentaphosphate [(p)ppGpp], also known as "magic spot", is a key sec-ond messenger inside bacterial cells as well as chloroplasts of plants and green algae. (p)ppGpp not only controls various stages of central dogma processes (replication, transcription, ribosome maturation and translation) and central metabolism but also regulates various physiological processes such as pathogen-esis, persistence, motility and competence. Under extreme conditions such as nutrient starvation, (p) ppGpp-mediated stringent response is crucial for the survival of bacterial cells. This mini-review high-lights some of the very recent progress on the key role of (p)ppGpp in bacterial growth control in light of cellular resource allocation and cell size regulation. We also briefly discuss some recent functional insights into the role of (p)ppGpp in plants and green algae from the angle of growth and development and further discuss several important open directions for future studies.(c) 2022 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. This is an open access article under the CC BY-NC-ND license (http://creative-commons.org/licenses/by-nc-nd/4.0/).
通讯作者：
Hong Yang<&wdkj&>Hong Yang Hong Yang Hong Yang
作者机构：
[Yang, Hong; Li, Xin-Yu; Mei, Cheng; Wulamu, Dilinuer] Cent China Normal Univ, Inst Entomol, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhan, Shuai; Luo, Xing-Yu; Huang, Yong-Ping] Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, CAS Key Lab Insect Dev & Evolutionary Biol, Inst Plant Physiol & Ecol,Shanghai Inst Biol Sci, Shanghai, Peoples R China.
通讯机构：
[Hong Yang; Hong Yang Hong Yang Hong Yang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan, China
关键词：
bacterial isolates;germ-free BSF larvae;growth and development;Hermetia illucens;promotive effects
摘要：
Graphical Abstract Analysis of the intestinal bacterial community composition of Hermetia illucens (black soldier fly, BSF) larvae (BSFL) with pyrosequencing showed that the dynamics of bacterial community composition among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, Dysgonomonas, Dysgonomonas, and Providencia were the relatively most abundant bacterial groups in the 1st‐ to 6th‐instar BSFL, respectively. Providencia, Citrobacter, Klebsiella, Ochrobactrum, and Dysgonomonas promoted the growth and development of BSF significantly compared with the germ‐free control by increasing the weight gain of larvae and pupae, as well as the prepupae rate and eclosion rate. In addition, Citrobacter, Klebsiella, and Providencia could shorten the life cycle of BSF significantly. Abstract Black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), is a prominent insect for the bioconversion of various organic wastes. As a saprotrophic insect, the BSF inhabits microbe‐rich environments. However, the influences of the intestinal microorganisms on BSF growth and development are not very clear. In this study, the dynamics of the intestinal bacterial community of BSF larvae (BSFL) were analyzed using pyrosequencing. Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria were the most prevalent bacterial phyla in the intestines of all larval instars. The dynamic changes in bacterial community compositions among different larval instars were striking at the genus level. Klebsiella, Clostridium, Providencia, and Dysgonomonas were the relatively most abundant bacteria in the 1st‐ to 4th‐instar BSFL, respectively. Dysgonomonas and Providencia also dominated the 5th‐ and 6th‐instar larvae, at ratios of 31.1% and 47.2%, respectively. In total, 148 bacterial strains affiliated with 20 genera were isolated on different media under aerobic and anaerobic conditions. Among them, 6 bacteria, BSF1–BSF6, were selected for further study. The inoculation of the 6 isolates independently into germ‐free BSFL feeding on an artificial diet showed that all the bacteria, except BSF4, significantly promoted BSF growth and development compared with the germ‐free control. Citrobacter, Dysgonomonas, Klebsiella, Ochrobactrum, and Providencia promoted BSF development significantly by increasing the weight gains of larvae and pupae, as well as increasing the prepupae and eclosion rates. In addition, Citrobacter, Klebsiella and Providencia shortened the BSF life cycle significantly. The results illustrate the promotive effects of intestinal bacteria on BSF growth and development.
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2023年140:104620 ISSN：0145-305X
通讯作者：
Xu-Sheng Liu
作者机构：
[Wang, Gui-Jie; Liu, Xu-Sheng; Wang, Jia-Lin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.;[Liu, Xu-Sheng] Cent China Normal Univ, Sch Life Sci, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Xu-Sheng Liu] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, 152 Luoyu Road, Wuhan, 430079, China
期刊：
Computational Biology and Bioinformatics,2023年11(1):13-18 ISSN：2330-8265
作者机构：
1College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, People’s Republic of China;2Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, People’s Republic of China
关键词：
Keywords Alternative Splicing;Intron Retention;Cis-elements;Rice;Drought Stress
摘要：
Pre-mRNAs splicing is one of the fundamental process which generates multiple transcripts from a single gene, contributing to transcriptome and proteome diversity. AS is regulated by the cooperation of trans-factors and cis-elements. In plants, extensive alternative splicing occurs not only in tissue-specific manner but also in response to stress conditions. Intron retention is the most predominant splicing type. However, the cis-elements regulating intron retention are still ambiguous in plants, especially under environmental stresses. This study aimed to elucidate the cis-elements underlying intron retention in plants under adverse enrironments. Using RNA-seq data of rice cultivars IRAT109 and ZS97 under drought environments, we compared the sequence characteristics between constitutive and retained introns. The results show that the main AS types include intron retention (IR), alternative acceptor sites (AA), alternative donor sites (AD) and cassette exon (exon skipping, ES). Among of them, IR was the prevelent pattern with frequencies of 30.8-31.2%. Motif analysis of 5' and 3' 200bp intron sequences found rich U(T) in the motifs for both constitutive and retained introns. By further analysis of base composition of sequences flanking splice sites, we detected a notable difference in U(T) content between introns and their neighboring exons in constitutive introns, but not in retained introns. The results in this study suggested that the lack of significant changes in U(T) content between retained introns and neighboring exons might be a potential cis feature of intron retention.
期刊：
JOURNAL OF PROTEOME RESEARCH,2023年22(9):2814-2826 ISSN：1535-3893
通讯作者：
Wan, CH
作者机构：
[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Ding, Yuhe; Gu, Jingyu; Peng, Zhao; Yan, Jiahao; Qin, Lu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
关键词：
zebrafish;early life stage;development;maternal-to-zygotic transition;mass spectrometry
摘要：
The early development of zebrafish (Danio rerio) is a complex and dynamic physiological process involving cell division, differentiation, and movement. Currently, the genome and transcriptome techniques have been widely used to study the embryonic development of zebrafish. However, the research of proteomics based on proteins that directly execute functions is relatively vacant. In this work, we apply label-free quantitative proteomics to explore protein profiling during zebrafish's embryogenesis, and a total of 5961 proteins were identified at 10 stages of zebrafish's early development. The identified proteins were divided into 11 modules according to weighted gene coexpression network analysis (WGCNA), and the characteristics between modules were significantly different. For example, mitochondria-related functions enriched the early development of zebrafish. Primordial germ cell-related proteins were identified at the 4-cell stage, while the eye development event is dominated at 5 days post fertilization (dpf). By combining with published transcriptomics data, we discovered some proteins that may be involved in activating zygotic genes. Meanwhile, 137 novel proteins were identified. This study comprehensively analyzed the dynamic processes in the embryonic development of zebrafish from the perspective of proteomics. It provided solid data support for further understanding of the molecular mechanism of its development.
通讯机构：
[Xiong, YZ; Lenhard, M ] U;[Xiong, YZ ] C;Cent China Normal Univ, Sch Life Sci, Luoyu Ave 152, Wuhan 430079, Peoples R China.;Univ Potsdam, Inst Biochem & Biol, Karl Liebknecht Str 24-25, D-14476 Potsdam, Germany.
关键词：
benzaldehyde;β-ocimene;Brassicaceae;Capsella;floral scent;pollinator attraction;quasi-isogenic line
摘要：
PREMISE OF THE STUDY: Floral scent, usually consisting of multiple compounds, is a complex trait and its role in pollinator attraction has received increasing attention. However, it is difficult to disentangle the effect of individual floral scent compounds due to the complexity of isolating the effect of single compounds by traditional methods. METHODS: Using available quasi-isogenic lines (qILs) generated as part of the original mapping of two floral scent volatile-related loci, CNL1 (benzaldehyde) and TPS2 (β-ocimene), in Capsella, we have generated four genotypes that should only systematically differ in these two compounds. Plants of the four genotypes were introduced into a common garden outside the natural range of C. rubella or C. grandiflora, with individuals of a self-compatible C. grandiflora line as pollen donors, whose different genetic background facilitates the detection of outcrossing events. Visitors to flowers of all five genotypes were compared, and the seeds set during the common-garden period were collected for high-throughput amplicon-based sequencing to estimate their outcrossing rates. KEY RESULTS: Benzaldehyde and β-ocimene emissions were detected in the floral scent of corresponding genotypes. While some pollinator groups showed specific visitation preferences depending on scent compounds, the outcrossing rates in seeds did not vary among the four scent-manipulated genotypes. CONCLUSIONS: The successful construction of scent-manipulated Capsella materials using qILs provides a powerful system to study the ecological effects of individual floral scent compounds under largely natural environments. In Capsella, individual BAld and β-ocimene emission may act as attractants for different types of pollinators. This article is protected by copyright. All rights reserved.
作者机构：
[Yu-tong ZHANG; Yang LIU] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;[Hang-wei LIU] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, P.R.China;[Song CAO] Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China<&wdkj&>Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, P.R.China;[Bin LI; Gui-rong WANG] State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, P.R.China
摘要：
Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities. Transient receptor potential (TRP) channels, which constitute a vast ion channel family, play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects. TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years. In this study, we identified 15 TRP gene loci encoding 26 transcripts in the genome of S. frugiperda and analyzed their expression profiles at different developmental stages. The results revealed that S. frugiperda possesses four TRPC genes, six TRPA genes, one TRPM gene, two TRPV genes, one TRPN gene, and one TRPML gene, while a canonical TRPP is absent. Moreover, the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system. The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45°C, and there is no significant desensitization after repeated stimuli within the same temperature range. Additionally, SfruTRPA1 is activated by certain natural chemicals, including allyl isothiocyanate (AITC) and cinnamaldehyde (CA). These findings provide valuable insights to the TRP genes in S. frugiperda.
作者机构：
[Fang, Yiwei; Peng, Meilin; Zhao, Kai; Liu, Chunyan] Huazhong Univ Sci & Technol, Inst Reprod Hlth, Tongji Med Coll, Wuhan 430030, Peoples R China.;[Zhang, Zheng; Yi, Guilin] Wuhan Prevent & Treatment Ctr Occupat Dis, Wuhan 430015, Peoples R China.;[Zhang, Zheng] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.;[Zhao, Yun; Sun, Guoqiang; Yang, Hui] Huazhong Univ Sci & Technol, Maternal & Child Hlth Hosp Hubei Prov, Tongji Med Coll, Dept Obstet, Wuhan 430070, Peoples R China.;[Zhao, Kai] Henan Inst Reprod Hlth Sci & Technol, NHC Key Lab Birth Defects Prevent, Zhengzhou 450000, Peoples R China.
通讯机构：
[Guilin Yi] W;[Kai Zhao] I;[Hui Yang] D;Department of Obstetrics, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China<&wdkj&>Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China<&wdkj&>NHC Key Laboratory of Birth Defects Prevention, Henan Institute of Reproduction Health Science and Technology, Zhengzhou, 450000, China<&wdkj&>Wuhan Prevention and Treatment Center for Occupational Diseases, Wuhan, 430015, China
作者机构：
[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Wan, Cuihong; Wan, CH; Zhang, Zheng; Li, Shengjie; Pan, Ni; Peng, Die; Wang, Shaohui] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Wan, CH ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Short open reading frame-encoded peptides (SEPs) are generally 2-100 amino acids in length and participate in various biological processes of the organism. The brain is the central hub of life activities, where different regions perform distinct functions. To characterize SEPs in brain regions, we analyzed SEPs in five mouse brain areas, including hippocampus, frontal cortex, temporal cortex, occipital cortex, and parietal cortex, with mass spectrometry-based proteomics. We obtained 1,095 proteins with less than 100 amino acids and identified 373 SEPs. Approximately 83% of these SEPs are reported for the first time. Half of them are encoded by ncRNA, and nearly one-third can find orthology across species. Specific SEPs were identified in each brain region. For example, IP_1018875 was identified in the frontal cortex, possibly related to autophagy and neuronal signaling. These results enrich the proteome of the mouse brain and help facilitate subsequent studies on the function of SEPs.
通讯机构：
[Qiu, BS ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
摘要：
Hypothetical chloroplast open reading frames (ycfs) are putative genes in the plastid genomes of photosynthetic eukaryotes. Many ycfs are also conserved in the genomes of cyanobacteria, the presumptive ancestors of present-day chloroplasts. The functions of many ycfs are still unknown. Here, we generated knock-out mutants for ycf51 (sll1702) in the cyanobacterium Synechocystis sp. PCC 6803. The mutants showed reduced photoautotrophic growth due to impaired electron transport between photosystem II (PSII) and PSI. This phenotype results from greatly reduced PSI content in the ycf51 mutant. The ycf51 disruption had little effect on the transcription of genes encoding photosynthetic complex components and the stabilization of the PSI complex. In vitro and in vivo analyses demonstrated that Ycf51 cooperates with PSI assembly factor Ycf3 to mediate PSI assembly. Furthermore, Ycf51 interacts with the PSI subunit PsaC. Together with its specific localization in the thylakoid membrane and the stromal exposure of its hydrophilic region, our data suggest that Ycf51 is involved in PSI complex assembly. Ycf51 is conserved in all sequenced cyanobacteria, including the earliest branching cyanobacteria of the Gloeobacter genus, and is also present in the plastid genomes of glaucophytes. However, Ycf51 has been lost from other photosynthetic eukaryotic lineages. Thus, Ycf51 is a PSI assembly factor that has been functionally replaced during the evolution of oxygenic photosynthetic eukaryotes. Chloroplast reading frame Ycf51 is a photosystem I assembly factor conserved in all sequenced cyanobacteria and glaucophyte plastids but lost from other photosynthetic eukaryotic lineages.
通讯机构：
[Haobin Zhao] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China<&wdkj&>Author to whom correspondence should be addressed.
期刊：
Journal of Environmental Chemical Engineering,2023年11(3):109717 ISSN：2213-3437
通讯作者：
Liao, XM;Zhang, Lizhi;Sun, HW;Zhang, LZ
作者机构：
[Liao, Xiaomei; Liu, Qianhui; Chen, Yangyang; Mei, Ting; Wang, Shaohui] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, Lizhi; Sun, Hongwei] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;[Zhang, Lizhi] Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China.;[Liao, XM] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, LZ; Sun, Hongwei] Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Liao, XM ; Zhang, LZ; Sun, HW ; Zhang, LZ ] ;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Cent China Normal Univ, Inst Environm & Appl Chem, Coll Chem, Key Lab Pesticide & Chem Biol,Minist Educ, Wuhan 430079, Peoples R China.;Shanghai Jiao Tong Univ, Sch Environm Sci & Engn, Shanghai 200240, Peoples R China.
关键词：
Sewage sludge;Ozonation;Zero-valent iron;Antibiotic resistance genes;Reactive oxygen species
摘要：
Wastewater treatment plants (WWTPs) are hotspots of antibiotic resistance genes (ARGs), and efficient technologies are needed to prevent the enrichment and spread of ARGs in municipal sludge. Herein, we demonstrated that oxalated zero-valent iron (OA-ZVIbm) catalyzed ozonation could effectively remove typical ARGs, tetG and sul1, from municipal sewage sludge, with the total abundance reduced by 3.02 and 2.00 log per gram sludge, respectively, surpassing the O-3 alone and O-3/ZVI(bm) counterparts. Meanwhile, the O-3/OA-ZVI(bm) treatment could simultaneously remove both intracellular and extracellular ARGs from the sludge, moreover, it also eliminated 1.83 log total Class 1 integron (total intI1), thus may suppress the horizontal gene transfer (HGT) potential of ARGs. The good performance of O-3/OA-ZVI(bm) was owing to the FeC2O4 center dot 2H(2)O shell of ZVI, which improved the accessibility of core iron species to catalyze the decomposition of O-3, producing more reactive oxygen species (ROSs) including center dot OH, center dot O-2(-), and O-1(2), for the removal of ARGs. This study provides a new method to control the dissemination of ARGs in sewage sludge.
作者：
Gomes, Marcelo Pedrosa;Pereira, Eduardo Gusmão;Qiu, Bao-Sheng;Juneau, Philippe
期刊：
FRONTIERS IN PLANT SCIENCE,2023年14:1131656 ISSN：1664-462X
通讯作者：
Gomes, M.P.
作者机构：
[Gomes, Marcelo Pedrosa] Laboratório de Fisiologia de Plantas sob Estresse, Departamento de Botânica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Paraná, Curitiba, Brazil;[Pereira, Eduardo Gusmão] Laboratório de Fisiologia do Estresse Abiótico, Instituto de Ciências Biológicas, Universidade Federal de Viçosa, Minas Gerais, Florestal, Brazil;[Qiu, Bao-Sheng] School of Life Sciences, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, Central China Normal University, Hubei, Wuhan, China;[Juneau, Philippe] Ecotoxicology of Aquatic Microorganisms Laboratory, EcotoQ, GRIL, TOXEN, Department of Biological Sciences, Université du Québec à Montréal, Montréal, Montréal, QC, Canada
通讯机构：
[Gomes, M.P.] L;Laboratório de Fisiologia de Plantas sob Estresse, Paraná, Brazil
摘要：
studies concerning that problematic, investigations aiming at understanding toxicological responses as well as the improvement of nature-based solutions to recover metal-impacted ecosystems are still needed. The planet has been faced with intensified anthropogenic activities, such as excessive disposal of mining waste and industrial and agricultural effluents. These contamination sources are responsible for the insertion of tons of toxic metals to environments; events demanding appropriate management actions. One of the most feasible solutions to reclaim toxic metals is the use of plants in phytoremediation technique. Although phytoremediation has proven as a green technology for metal removal from low-to-moderate contaminated sites, the selection of plant species as well as of supporting strategies aiming to enhance phytoremediation efficiency merit attention (Gomes et al., 2021).Due to their high biomass yields, fast growth, adaptation strategies to infertile soils and successive short regrowth after harvest, grasses have been indicated as prospective candidates for metal phytoremediation. In their review, Rabêlo et al. synthesized information on mechanisms involved in uptake, accumulation and tolerance to toxic metals, identified suitable grass species for phytoremediation processes, described strategies used to improve their phytoremediation efficiency and, mainly, pointed out to the advantages and disadvantages of using grass species in phytoremediation. Although gras...
通讯机构：
[Xinmin Lu] S;State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University , Wuhan 430070 , China<&wdkj&>Institute of Invasion Biology and Agriculture Ecological Safety, Huazhong Agricultural University , Wuhan 430070 , China<&wdkj&>College of Plant Sciences and Technology, Huazhong Agricultural University , Wuhan 430070 , China
摘要：
Soil biota, as legacy effects of previous species in natural ecosystems, profoundly affects plant performance in new habitats and, in turn, plant community. However, how soil biota, as legacy effects of agricultural crops, affects the likelihood of establishment of exotic and native plants in newly abandoned farmland remains poorly understood, which may hinder effective management of agricultural weeds. Here, we grew 58 plant species (28 exotic species and 30 native species) common in Central China in sterilized vs. nonsterilized soils collected from a newly abandoned maize field. We (i) estimated the effects of soil biota on plant shoot, root and total mass, (ii) explored the dependence of soil effects on the plants' phylogenetic distance to maize, origin (native vs. exotic) and life history (annual vs. perennial) and (iii) tested which plant traits could predict soil effects. Soil biota, in general, decreased plant mass, suggesting a dominant role of enemies. The effect of the soil biota on plant total mass was unrelated to the phylogenetic distance of the plants to maize and decreased linearly with increasing plant intrinsic growth ability. Moreover, the soil biota on average had greater negative impacts on the total mass of exotic plants, particularly perennial species, than on that of native plants. Our results suggest that plant intrinsic growth ability, rather than phylogenetic relatedness, is a reliable predictor of soil effects. Additionally, native plants can benefit from plant-soil interactions in competing with exotic perennial plants when initially established in newly abandoned farmlands.
作者机构：
[Wang, Yao; Zheng, Yong; He, Shao-Ping; Xu, Shang-Wei; Li, Xue-Bao; Li, XB; Li, Yang; Zheng, Y] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Li, Li; Li, L] Huazhong Agr Univ, Coll Biomed & Hlth, Wuhan 430070, Peoples R China.;[Li, Li; Li, L] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.;[Wang, Yao] Sichuan Agr Univ, Maize Res Inst, Wenjiang 611130, Sichuan, Peoples R China.
通讯机构：
[Li, XB ; Zheng, Y] C;[Li, L ] H;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;Huazhong Agr Univ, Coll Biomed & Hlth, Wuhan 430070, Peoples R China.;Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.
摘要：
Transcription factors GhERF108 and GhARF7 interact to establish ethylene-auxin crosstalk, which activates downstream secondary cell wall (SCW)-related genes to facilitate fiber SCW formation in cotton. Phytohormones play indispensable roles in plant growth and development. However, the molecular mechanisms underlying phytohormone-mediated regulation of fiber secondary cell wall (SCW) formation in cotton (Gossypium hirsutum) remain largely underexplored. Here, we provide mechanistic evidence for functional interplay between the APETALA2/ethylene response factor (AP2/ERF) transcription factor GhERF108 and auxin response factors GhARF7-1 and GhARF7-2 in dictating the ethylene-auxin signaling crosstalk that regulates fiber SCW biosynthesis. Specifically, in vitro cotton ovule culture revealed that ethylene and auxin promote fiber SCW deposition. GhERF108 RNA interference (RNAi) cotton displayed remarkably reduced cell wall thickness compared with controls. GhERF108 interacted with GhARF7-1 and GhARF7-2 to enhance the activation of the MYB transcription factor gene GhMYBL1 (MYB domain-like protein 1) in fibers. GhARF7-1 and GhARF7-2 respond to auxin signals that promote fiber SCW thickening. GhMYBL1 RNAi and GhARF7-1 and GhARF7-2 virus-induced gene silencing (VIGS) cotton displayed similar defects in fiber SCW formation as GhERF108 RNAi cotton. Moreover, the ethylene and auxin responses were reduced in GhMYBL1 RNAi plants. GhMYBL1 directly binds to the promoters of GhCesA4-1, GhCesA4-2, and GhCesA8-1 and activates their expression to promote cellulose biosynthesis, thereby boosting fiber SCW formation. Collectively, our findings demonstrate that the collaboration between GhERF108 and GhARF7-1 or GhARF7-2 establishes ethylene-auxin signaling crosstalk to activate GhMYBL1, ultimately leading to the activation of fiber SCW biosynthesis.
作者机构：
[Xiao, Yutao; Liu, Conghui; Liu, Zhenxing; Fan, Wei; Jin, Minghui; Wang, Ping; Liu, Bo; Wu, Chao; Wu, Shigang; Liu, Hangwei; He, Yuan; Chakrabarty, Swapan; Zheng, Weigang] Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Key Lab Gene Editing Technol Hainan, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,M, Shenzhen, Peoples R China.;[Jin, Minghui; Wu, Kongming; He, Yuan; Li, Xiaokang] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China.;[Yuan, Haibin; Zheng, Weigang] Jilin Agr Univ, Coll Agron, Changchun 130118, Peoples R China.;[Liu, Conghui] Univ Hong Kong, Dept Clin Oncol, Hong Kong 999077, Peoples R China.;[Liu, Kaiyu] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Kongming Wu] T;[Wei Fan; Yutao Xiao] S;The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China<&wdkj&>Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Gene Editing Technologies (Hainan), Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China
摘要：
BACKGROUND: The black cutworm, Agrotis ipsilon, is a serious global underground pest. Its distinct phenotypic traits, especially its polyphagy and ability to migrate long distances, contribute to its widening distribution and increasing difficulty of control. However, knowledge about these traits is still limited. RESULTS: We generated a high-quality chromosome-level assembly of A. ipsilon using PacBio and Hi-C technology with a contig N50 length of ~ 6.7Mb. Comparative genomic and transcriptomic analyses showed that detoxification-associated gene families were highly expanded and induced after insects fed on specific host plants. Knockout of genes that encoded two induced ABC transporters using CRISPR/Cas9 significantly reduced larval growth rate, consistent with their contribution to host adaptation. A comparative transcriptomic analysis between tethered-flight moths and migrating moths showed expression changes in the circadian rhythm gene AiCry2 involved in sensing photoperiod variations and may receipt magnetic fields accompanied by MagR and in genes that regulate the juvenile hormone pathway and energy metabolism, all involved in migration processes. CONCLUSIONS: This study provides valuable genomic resources for elucidating the mechanisms involved in moth migration and developing innovative control strategies.
