摘要：
Significance Statement This study has revealed an important role of OsTIL1 in maintaining the cell membrane integrity by regulating the 18:3‐containing glycerolipids biosynthesis and reducing reactive oxygen species damage under cold stress in rice seedlings. SUMMARY Lipocalins constitute a conserved protein family that binds to and transports a variety of lipids while fatty acid desaturases (FADs) are required for maintaining the cell membrane fluidity under cold stress. Nevertheless, it remains unclear whether plant lipocalins promote FADs for the cell membrane integrity under cold stress. Here, we identified the role of OsTIL1 lipocalin in FADs‐mediated glycerolipid remodeling under cold stress. Overexpression and CRISPR/Cas9 mediated gene edition experiments demonstrated that OsTIL1 positively regulated cold stress tolerance by protecting the cell membrane integrity from reactive oxygen species damage and enhancing the activities of peroxidase and ascorbate peroxidase, which was confirmed by combined cold stress with a membrane rigidifier dimethyl sulfoxide or a H2O2 scavenger dimethyl thiourea. OsTIL1 overexpression induced higher 18:3 content, and higher 18:3/18:2 and (18:2 + 18:3)/18:1 ratios than the wild type under cold stress whereas the gene edition mutant showed the opposite. Furthermore, the lipidomic analysis showed that OsTIL1 overexpression led to higher contents of 18:3‐mediated glycerolipids, including galactolipids (monoglactosyldiacylglycerol and digalactosyldiacylglycerol) and phospholipids (phosphatidyl glycerol, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidyl serine and phosphatidyl inositol) under cold stress. RNA‐seq and enzyme linked immunosorbent assay analyses indicated that OsTIL1 overexpression enhanced the transcription and enzyme abundance of four ω‐3 FADs (OsFAD3‐1/3‐2, 7, and 8) under cold stress. These results reveal an important role of OsTIL1 in maintaining the cell membrane integrity from oxidative damage under cold stress, providing a good candidate gene for improving cold tolerance in rice.
摘要：
Eukaryotic DNA is packaged into chromatin in the nucleus, restricting the binding of transcription factors (TFs) to their target DNA sites. FOXA1 functions as a pioneer TF to bind condensed chromatin and initiate the opening of local chromatin for gene expression. However, the principles of FOXA1 recruitment and how it subsequently unpacks the condensed chromatin remain elusive. Here, we revealed that FOXA1 intrinsically forms submicron-sized condensates through its N- and C-terminal intrinsically disordered regions (IDRs). Notably, both IDRs enable FOXA1 to dissolve the condensed chromatin. In addition, the DNA-binding capacity of FOXA1 contributes to its ability to both form condensates and dissolve condensed chromatin. Further genome-wide investigation showed that IDRs enable FOXA1 to bind and unpack the condensed chromatin to regulate the proliferation and migration of breast cancer cells. This work provides a principle of how pioneer TFs function to initiate competent chromatin states using their IDRs.
期刊：
Journal of Plant Research,2024年:1-13 ISSN：0918-9440
通讯作者：
Qin-Zheng Hou
作者机构：
[Nurbiye Ehmet; Xiang Zhao; Tai-Hong Wang; Yi-Ping Zhang; Kun Sun] College of Life Sciences, Northwest Normal University, Lanzhou, 730070, Gansu, China;[Nurbiye Ehmet] College of Life Sciences, Central China Normal University, Wuhan, 430079, Hubei, China;[Qin-Zheng Hou] College of Life Sciences, Northwest Normal University, Lanzhou, 730070, Gansu, China. hou_qzh@nwnu.edu.cn
通讯机构：
[Hou, Qin-Zheng] C;College of Life Sciences, Northwest Normal University, Lanzhou, 730070, Gansu, China.
摘要：
The intervention of nectar robbers in plant pollination systems will cause some pollinators to modify their foraging behavior to act as secondary robbers, consequently adopting a mixed foraging strategy. The influence of nectar robbing on pollinator behavior may be affected by spatio-temporal difference of robbing intensity, and consequently, may have different effects on the pollination of host plants. However, whether and how the nectar robbing might influence pollinators under different robbing intensity still needs further investigation. In this study, Symphytum officinale was used to detect the effect of nectar robbers on pollinators under different robbing intensity as well as their effects on plant reproductive success. Six robbing levels and three bumblebees with mixed foraging behaviors were used to evaluate the effect of different robbing intensity on pollinator behavior, visitation rate, flower longevity and pollen deposition. Our results indicated that the robbing rate increased gradually with the proportion of robbed flowers, but which did not affect the frequency of legitimate visits. The increase of robbing rate promoted the corolla abscission, and then enhanced the self-pollen deposition, but which had no significant effect on cross-pollen deposition. These results indicate that the overall fitness of S. officinale was improved by combined self and cross-pollination modes when visited by both pollinators and nectar robbers simultaneously. Although nectar robbing is not uncommon, its consequences for pollination in the interaction web have not been well studied. Our results emphasize the significance of indirect impacts in mediating the adaptive outcomes of species interactions.
期刊：
Science of The Total Environment,2024年922:171299 ISSN：0048-9697
通讯作者：
Chen, Mingqing
作者机构：
[Xu, Ke; Jiang, Mengling; Wang, Shuxin; Han, Qi; Gao, Xiao; Du, Wanting; Wang, Yunyi] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China;[Chen, Mingqing] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China. Electronic address: chenmq@mail.ccnu.edu.cn
通讯机构：
[Chen, Mingqing] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China. Electronic address:
摘要：
After aging in the environment, some nanoplastics will carry different charges and functional groups, thereby altering their toxicological effects. To evaluate the potential impact of aging of nanoplastics on the mammalian reproductive system, we exposed C57BL/6 male mice to a dose of 5mg/kg/d polystyrene nanoparticles (PS-NPs) with different functional groups (unmodified, carboxyl functionalized and amino functionalized) for 45days for this study. The results suggest that PS-NPs with different functional groups triggered oxidative stress, a decreased in the testis index, disruption of the outer wall of the seminiferous tubules, reduction in the number of spermatogonia cells and sperm counts, and an increased in sperm malformations. We performed GO and KEGG enrichment analysis on the differentially expressed proteins, and found they were mainly enriched in protein transport, RNA splicing and mTOR signaling. We confirmed that the PI3K-AKT-mTOR pathway is over activated, which may lead to reduction of spermatogonia stem cells by over differentiation. Strikingly, PS-NPs with functional group modifications are more toxic than those of unmodified polystyrene, and that PS-NPs with positively charged amino modifications are the most toxic. This study provides a new understanding for correctly evaluating the toxicological effects of plastic aging, and of the mechanism responsible for the reproductive toxicity caused by nanoplastics.
作者机构：
[Yang, Xiao-Chen] College of Biology and Environmental Sciences, Jishou University, Jishou 416000 , Hunan Province, China;[Lv, Xiao-Wen; Huang, Shuang-Quan] Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , Wuhan 430079, China;Department of Ecology, College of Life Sciences, Wuhan University , Wuhan 430072, China;[Feng, Hui-Hui] Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , Wuhan 430079, China<&wdkj&>Department of Ecology, College of Life Sciences, Wuhan University , Wuhan 430072, China
通讯机构：
[Shuang-Quan Huang] I;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , Wuhan 430079, China
摘要：
BACKGROUNDS AND AIMS: The hypothesis that plants evolve features that protect accessible pollen from consumption by flower visitors remains poorly understood. METHODS: To explore potential chemical defenses against pollen consumption, we examined the pollinator assemblage, foraging behaviour, visitation frequency and pollen transfer efficiency in Rhododendron molle, a highly toxic shrub containing Rhodojaponin III. Nutrient (protein and lipid) and toxic components in pollen and other tissues were measured. KEY RESULTS: Overall in the five populations, floral visits by butterflies and bumblebees were relatively more frequent than visits by honeybees. All foraged for nectar but not pollen. Butterflies did not differ from bumblebees in the amount of pollen removed per visit, but deposited more pollen per visit. Pollination experiments indicated that R. molle was self-compatible, but both fruit and seed production were pollen limited. Our analysis indicated that the pollen was not protein-poor and had a higher concentration of the toxic compound Rhodojaponin III than petals and leaves, which compound was undetectable in nectar. CONCLUSION: Pollen toxicity in Rhododendron flowers may discourage pollen robbers (bees) from taking the freely accessible pollen grains, while the toxin-free nectar rewards effective pollinators, promoting pollen transfer. This preliminary study supports the hypothesis that chemical defense in pollen would be likely to evolve in species without physical protection from pollinivores.
期刊：
Journal of Environmental Management,2024年351:119729 ISSN：0301-4797
通讯作者：
Yang, S
作者机构：
[Peng, Kaida; Yang, Shao; Yang, Hongyan; Xu, Min; Cheng, Hu; Liu, Yi; Yang, S; Liu, Pan; Liu, Xiaofeng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
通讯机构：
[Yang, S ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Peoples R China.
关键词：
Anthropogenic activities;Bloom-forming cyanobacterial genera;Climate change;Driving factors;Sedimentary ancient DNA;Thresholds
摘要：
Cyanobacterial blooms pose a global environmental concern, with various genera contributing to their formation. The harmfulness of cyanobacterial blooms varies depending on the specific genus, yet the factors triggering their formation remain incompletely understood. This study conducted qPCR of sediment DNA in Lake Erhai to reconstruct the historical succession of three common bloom-forming cyanobacterial genera (i.e., Microcystis, Dolichospermum, and Aphanizomenon). The driving factors and their corresponding thresholds were identified, and human activities related to driving factors were evaluated. The results revealed two successions in the past century. The first succession transitioned from Aphanizomenon (1902-1978) to Microcystis and Dolichospermum (1978-1999), driven by TN:TP and TP. The second succession shifted from Microcystis and Dolichospermum (1978-1999) to Microcystis (1999-2010), driven by TP, TN:TP, and temperature. The thresholds of TP and TN:TP for the Microcystis bloom were 0.023mg/L and 17, respectively. TN:TP was significantly influenced by domestic pollution and crop farming in both successions, while TP was significantly impacted by domestic pollution in the first succession and by pollution from crop and dairy farming in the second succession. These results shed light on the underlying mechanism responsible for the blooms of various cyanobacterial genera and could serve as a valuable reference for effectively preventing and controlling nutrient input in the watershed.
作者机构：
[Wang, Dong; Wang, D; Cao, Dandan] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yang, Yongqing] Chongqing Normal Univ, Coll Life Sci, Chongqing 401331, Peoples R China.;[Wang, Dong; Wang, D] Shaanxi Univ Technol, Bioresources Key Lab Shaanxi Prov, Hanzhong 723001, Shaanxi, Peoples R China.
通讯机构：
[Wang, D ] C;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Shaanxi Univ Technol, Bioresources Key Lab Shaanxi Prov, Hanzhong 723001, Shaanxi, Peoples R China.
摘要：
Invasive plants have been shown to alter ecosystem functions. However, there is limited information available on the effect of the invasive species litter input on native litter decomposition and home-field advantage (HFA, increased decomposition at a home environment compared to a guest environment). To investigate this, we conducted a field experiment using leaves of native Trapa natans and Typha orientalis with and without invasive species Alternanthera philoxeroides and then incubated them at T. natans and T. orientalis habitats in a eutrophic lake for 50 days. In control and treatment, the decomposition and N release rate of T. natans were significantly higher than that in T. orientalis species litter at two habitats, indicating no HFA occurrence. A. philoxeroides litter input promoted the decomposition and N decay rate of T. orientalis, not T. natans. The mean decomposition rate of T. orientalis in control and treatment were 0.0138 and 0.0342 day-1; for T. natans, it was 0.0703 and 0.0754 day-1, respectively. The mean N decay rate of T. orientalis in control and treatment were 0.0235 and 0.0468 day-1; for T. natans, it was 0.0511 and 0.0544 day-1, respectively. Invasive species presence increased microbial respiration rate of T. orientalis, but not for T. natans species litter in two habitats. These results suggested that A. philoxeroides litter input accelerated low quality litter decomposition though increased microbial activity. However, it did not influence the HFA performance, which may be closely related to the lake eutrophication.
摘要：
Upland cotton, the mainly cultivated cotton species in the world, provides over 90% of natural raw materials (fibers) for the textile industry. The development of cotton fibers that are unicellular and highly elongated trichomes on seeds is a delicate and complex process. However, the regulatory mechanism of fiber development is still largely unclear in detail. In this study, we report that a homeodomain-leucine zipper (HD-ZIP) IV transcription factor, GhHOX4, plays an important role in fiber elongation. Overexpression of GhHOX4 in cotton resulted in longer fibers, while GhHOX4-silenced transgenic cotton displayed a "shorter fiber" phenotype compared with wild type. GhHOX4 directly activates two target genes, GhEXLB1D and GhXTH2D, for promoting fiber elongation. On the other hand, phosphatidic acid (PA), which is associated with cell signaling and metabolism, interacts with GhHOX4 to hinder fiber elongation. The basic amino acids KR-R-R in START domain of GhHOX4 protein are essential for its binding to PA that could alter the nuclear localization of GhHOX4 protein, thereby suppressing the transcriptional regulation of GhHOX4 to downstream genes in the transition from fiber elongation to secondary cell wall (SCW) thickening during fiber development. Thus, our data revealed that GhHOX4 positively regulates fiber elongation, while PA may function in the phase transition from fiber elongation to SCW formation by negatively modulating GhHOX4 in cotton.
期刊：
BRIEFINGS IN BIOINFORMATICS,2024年25(2) ISSN：1467-5463
通讯作者：
Yunjie Zhao<&wdkj&>Yunhui Peng
作者机构：
[Xu, Wang; Peng, Yunhui; Zhang, Houfang; Zhao, Yunjie] Institute of Biophysics and Department of Physics, Central China Normal University, Wuhan 430079, China;[Guo, Wenhan] Computational Science Program, University of Texas at El Paso, El Paso, TX 79902, USA;[Jiang, Lijun] Hubei Key Laboratory of Genetic Regulation & Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
通讯机构：
[Yunjie Zhao; Yunhui Peng] I;Institute of Biophysics and Department of Physics, Central China Normal University , Wuhan 430079 , China
关键词：
epigenetic regulation;histone cancer mutation;histone/nucleosome interaction;interaction network;nucleosome binding mode
摘要：
Nucleosomes represent hubs in chromatin organization and gene regulation and interact with a plethora of chromatin factors through different modes. In addition, alterations in histone proteins such as cancer mutations and post-translational modifications have profound effects on histone/nucleosome interactions. To elucidate the principles of histone interactions and the effects of those alterations, we developed histone interactomes for comprehensive mapping of histone-histone interactions (HHIs), histone-DNA interactions (HDIs), histone-partner interactions (HPIs) and DNA-partner interactions (DPIs) of 37 organisms, which contains a total of 3808 HPIs from 2544 binding proteins and 339 HHIs, 100 HDIs and 142 DPIs across 110 histone variants. With the developed networks, we explored histone interactions at different levels of granularities (protein-, domain- and residue-level) and performed systematic analysis on histone interactions at a large scale. Our analyses have characterized the preferred binding hotspots on both nucleosomal/linker DNA and histone octamer and unraveled diverse binding modes between nucleosome and different classes of binding partners. Last, to understand the impact of histone cancer-associated mutations on histone/nucleosome interactions, we complied one comprehensive cancer mutation dataset including 7940 cancer-associated histone mutations and further mapped those mutations onto 419,125 histone interactions at the residue level. Our quantitative analyses point to histone cancer-associated mutations' strongly disruptive effects on HHIs, HDIs and HPIs. We have further predicted 57 recurrent histone cancer mutations that have large effects on histone/nucleosome interactions and may have driver status in oncogenesis.
作者：
Meng Zhang;Li-Bing Jia;Susanne S Renner;Zhi-Xi Tian;Xiao-An Wang;...
期刊：
国家科学评论(英文),2024年 ISSN：2095-5138
通讯作者：
Susanne S Renner<&wdkj&>Huan-Li Xu<&wdkj&>Shuang-Quan Huang
作者机构：
[Meng Zhang; Li-Bing Jia; Zhi-Xi Tian; Xiao-An Wang; Shuang-Quan Huang] Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , Wuhan 430079 , China;[Jiang-Yun Gao] State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan, Yunnan University , Kunming 650091 , China;[Yi- Bo Luo] State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences , Beijing 100093 , China;[Antonio J C Aguiar] Department of Zoology, University of Brasília , Distrito Federal 70910 900 , Brazil;[Susanne S Renner] Department of Biology, Washington University , St. Louis , MO 63130 , USA
通讯机构：
[Susanne S Renner; Huan-Li Xu] D;[Shuang-Quan Huang] I;Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University , Wuhan 430079 , China<&wdkj&>Department of Biology, Washington University , St. Louis , MO 63130 , USA<&wdkj&>Department of Entomology, College of Plant Protection, China Agricultural University , Beijing 100193 , China
摘要：
Meng Zhang, Li-Bing Jia, Susanne S Renner, Zhi-Xi Tian, Xiao-An Wang, Jiang-Yun Gao, Yi- Bo Luo, Antonio J C Aguiar, Huan-Li Xu, Shuang-Quan Huang; Multipartite
期刊：
Trends in Pharmacological Sciences,2024年45(3):193-196 ISSN：0165-6147
通讯作者：
Jinrong Min
作者机构：
[Li, Yanxi; Huang, Yunyuan] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei, China;[Min, Jinrong] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei, China. Electronic address: minjinrong@ccnu.edu.cn
通讯机构：
[Jinrong Min] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, Hubei, China
摘要：
The PWWP domain binds to both histone and DNA of a nucleosome in a bivalent way. PWWP domain-containing proteins are involved in different biological processes, and their aberrant expression is implicated in various human diseases. Here, we discuss the recent developments and challenges in targeting the PWWP domain for therapeutic intervention.
关键词：
Antibiotic alternatives;Antibiotic resistance genes;Layer feces;Microbiome;Mobile genetic elements
摘要：
Although antibiotic alternatives are widely used in livestock and poultry breeding industry after in-feed antibiotics ban, their intervention effects on antibiotic resistance genes (ARGs) in these food animals' feces remain poorly understood. Here effects of fructooligosaccharide (FOS) and astragalus polysaccharide (APS), as typical antibiotic alternatives in China, on ARGs in layer feces were estimated by performing metagenomic sequencings and fluorescence quantitative PCR. Fructooligosaccharide significantly reduced sum abundance of ARGs and mobile genetic elements (MGEs) by increasing Lactobacillus clones and reducing Escherichia clones which had relatively higher abundances of ARG subtypes and MGE subtypes in layer feces. However, at least parts of core ARGs and MGEs categories were not reduced by FOS, such as aminoglycosides- and tetracyclines-resistant genes, Tn916, Integrase, and so on. MGEs and microbiome, especially Escherichia genus and Lactobacillus genus, were the key factors affecting ARGs' sum abundance. MGEs had a higher correlation coefficient with ARGs' sum abundance than Escherichia genus and Lactobacillus genus. These findings firstly reveal the defects of antibiotic alternatives in controlling bacterial resistance in livestock and poultry breeding after in-feed antibiotics ban, and more strategies are needed to control pollutions and risks of core ARGs and MGEs in food animals' feces under a special environment.
摘要：
Graphene-based nanomaterials (GBNs) consist of a single or few layers of graphene sheets or modified graphene including pristine graphene, graphene nanosheets (GNS), graphene oxide (GO), reduced graphene oxide (rGO), as well as graphene modified with various functional groups or chemicals (e.g., hydroxyl, carboxyl, and polyethylene glycol), which are frequently used in industrial and biomedical applications owing to their exceptional physicochemical properties. Given the widespread production and extensive application of GBNs, they can be disseminated in a wide range of environmental mediums, such as air, water, food, and soil. GBNs can enter the human body through various routes such as inhalation, ingestion, dermal penetration, injection, and implantation in biomedical applications, and the majority of GBNs tend to accumulate in the respiratory system. GBNs inhaled and substantially deposited in the human respiratory tract may impair lung defenses and clearance, resulting in the formation of granulomas and pulmonary fibrosis. However, the specific toxicity of the respiratory system caused by different GBNs, their influencing factors, and the underlying mechanisms remain relatively scarce. This review summarizes recent advances in the exposure, metabolism, toxicity and potential mechanisms, current limitations, and future perspectives of various GBNs in the respiratory system.
作者机构：
[Cong, Shengbo; Wan, Peng; Xu, Dong; Wang, Ling] Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Crop Disease, Insect Pests and Weeds Control, Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, 430064, China;[Xu, Min; Liu, Kaiyu; He, Lu] School of Life Science, Central China Normal University, Wuhan, 430079, China;[Wei, Wei] Applied Biotechnology Center, Wuhan University of Bioengineering, Wuhan, 430415, China
摘要：
BACKGROUND: With the increasing incidence of pest resistance to transgenic crops producing Bacillus thuringiensis (Bt) proteins in the field, elucidating the molecular basis of resistance is important for monitoring, delaying, and countering pest resistance. Previous work revealed that mutation or downregulated expression of the cadherin gene (PgCad1) is associated with pink bollworm (Pectinophora gossypiella) resistance to Cry1Ac, and twenty mutant PgCad1 alleles (r1-r20) were characterized. Here, we tested the hypothesis that the ABC transporter PgABCC2 is a functional receptor for the Bt toxin Cry1Ac and that a mutation is associated with resistance. RESULTS: We identified and characterized the first resistance allele (r(C2) ) of PgABCC2 in the laboratory-selected Cry1Ac resistant strain AQ-C2 of pink bollworm. The r(C2) allele had a one-base deletion in exon20, resulting in a frameshift and the introduction of a premature stop codon. This resulting PgABCC2 protein had a truncated C-terminus, including the loss of the NBD2 domain. AQ-C2 exhibited 20.2-fold greater resistance to Cry1Ac than the susceptible strain, and its inheritance of Cry1Ac resistance was recessive and genetically linked to PgABCC2. When produced in cultured insect cells, recombinant wildtype and r(C2) mutant PgABCC2 proteins localized within the cell plasma membrane, although substantial cytoplasmic retention was also observed for the mutant protein, while the mutant PgABCC2 caused a 13.9-fold decrease in Cry1Ac toxicity versus the wildtype PgABCC2. CONCLUSIONS: PgABCC2 is a functional receptor of Cry1Ac and the loss of its carboxyl terminus (including its NBD2 domain) confers low-level resistance to Cry1Ac in both larvae and in cultured cells.
作者机构：
[Jing-Bo Zhang] Hubei Key Laboratory of Genetic Regulation and Integrative Biology,School of Life Sciences,Central China Normal University,Wuhan 430079,Hubei,China;[Jing-Bo Zhang] College of Life Sciences,Xinjiang Normal University,Urumqi 830017,Xinjiang,China;[Yang Li; Yong Zheng; Xue-Bao Li; Fan Cheng; Shi-Peng Zhang; Yao Wang] 华中师范大学
通讯机构：
[Yang Li; Xue-Bao Li] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan 430079, Hubei, China
摘要：
Drought stress impairs crop growth and development. BEL1-like family transcription factors may be involved in plant response to drought stress, but little is known of the molecular mechanism by which these proteins regulate plant response and defense to drought stress. Here we show that the BEL1-like transcription factor GhBLH5-A05 functions in cotton（Gossypium hirsutum） response and defense to drought stress. Expression of GhBLH5-A05 in cotton was induced by drought stress. Overexpression of GhBLH5-A05 in both Arabidopsis and cotton increased drought tolerance, whereas silencing GhBLH5-A05 in cotton resulted in elevated sensitivity to drought stress. GhBLH5-A05 binds to cis elements in the promoters of GhRD20-A09 and GhDREB2C-D05 to activate the expression of these genes. GhBLH5-A05 interacted with the KNOX transcription factor GhKNAT6-A03. Co-expression of GhBLH5-A05 and GhKNAT6-A03 increased the transcription of GhRD20-A09 and GhDREB2C-D05. We conclude that GhBLH5-A05 acts as a regulatory factor with GhKNAT6-A03 functioning in cotton response to drought stress by activating the expression of the drought-responsive genes Gh RD20-A09 and Gh DREB2C-D05.
摘要：
Water molecules are actively involved in many catalytic oxidation processes, which require the construction of highly active sites for their activation to accelerate the reaction rate, especially over non-noble metal catalysts. Herein, K species is embeded into the natural 2*2 channel of alpha-MnO2 by a hydrothermal coupled molten salt method, which would make these K species behave in an electron-rich state and provide more electrons for the activation of water molecules. Compared with surface K modification (namely, the electron-deficient K species), channel K confinement can lower the activation energy barrier of H2O dissociation on alpha-MnO2 to generate hydroxyl species with more nucleophilic oxygen atoms, contributing to the superior HCHO catalytic oxidation activity with a fourfold enhancement. The internal relationship among the confined channel, K species, and catalytic performance is systematically elucidated at the molecular level. This work offers a new ion confinement method and opens up new avenues to construct electron-rich metal sites with channel structures for the activation of water molecules.
作者机构：
[Wen-Wen Wang; Pei Xiong; Xu-Sheng Liu; Yu-Feng Wang] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China;[Jia-Lin Wang] Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, China. jlwang@ccnu.edu.cn
通讯机构：
[Jia-Lin Wang] H;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, China
摘要：
Gut bacteria are beneficial to the host, many of which must be passed on to host offspring. During metamorphosis, the midgut of holometabolous insects undergoes histolysis and remodeling, and thus risks losing gut bacteria. Strategies employed by holometabolous insects to minimize this risk are obscure. How gut bacteria affect host insects after entering the hemocoel and causing opportunistic infections remains largely elusive. We used holometabolous Helicoverpa armigera as a model and found low Lactobacillus load, high level of a C-type lectin (CTL) gene CD209 antigen-like protein 2 (CD209) and its downstream lysozyme 1 (Lys1) in the midgut of the wandering stage. CD209 or Lys1 depletion increased the load of midgut Lactobacillus, which further translocate to the hemocoel. In particular, CD209 or Lys1 depletion, injection of Lactobacillus plantarum, or translocation of midgut L. plantarum into the hemocoel suppressed 20-hydroxyecdysone (20E) signaling and delayed pupariation. Injection of L. plantarum decreased triacylglycerol and cholesterol storage, which may result in insufficient energy and 20E available for pupariation. Further, Lysine-type peptidoglycan, the major component of gram-positive bacterial cell wall, contributed to delayed pupariation and decreased levels of triacylglycerols, cholesterols, and 20E, in both H. armigera and Drosophila melanogaster. A mechanism by which (Lactobacillus-induced) opportunistic infections delay insect metamorphosis was found, namely by disturbing the homeostasis of lipid metabolism and reducing 20E production. Moreover, the immune function of CTL − Lys was characterized for insect metamorphosis by maintaining gut homeostasis and limiting the opportunistic infections.
