摘要：
To fully harness the potential of laccase in the efficient decolorization and detoxification of single and mixed dyes with diverse chemical structures, we carried out a systematic study on the decolorization and detoxification of single and mixed dyes using a crude laccase preparation obtained from a white-rot fungus strain, Pleurotus eryngii. The crude laccase preparation showed efficient decolorization of azo, anthraquinone, triphenylmethane, and indigo dyes, and the reaction rate constants followed the order Remazol Brilliant Blue R > Bromophenol blue > Indigo carmine > New Coccine > Reactive Blue 4 > Reactive Black 5 > Acid Orange 7 > Methyl green. This laccase preparation exhibited notable tolerance to SO(4)(2-) salts such as MnSO(4), MgSO(4), ZnSO(4), Na(2)SO(4), K(2)SO(4), and CdSO(4) during the decolorization of various types of dyes, but was significantly inhibited by Cl(-) salts. Additionally, this laccase preparation demonstrated strong tolerance to some organic solvents such as glycerol, ethylene glycol, propanediol, and butanediol. The crude laccase preparation demonstrated the efficient decolorization of dye mixtures, including azo + azo, azo + anthraquinone, azo + triphenylmethane, anthraquinone + indigo, anthraquinone + triphenylmethane, and indigo + triphenylmethane dyes. The decolorization kinetics of mixed dyes provided preliminary insight into the interactions between dyes in the decolorization process of mixed dyes, and the underlying reasons and mechanisms were discussed. Importantly, the crude laccase from Pleurotus eryngii showed efficient repeated-batch decolorization of single-, two-, and four-dye mixtures. This crude laccase demonstrated high stability and reusability in repeated-batch decolorization. Furthermore, this crude laccase was efficient in the detoxification of different types of single dyes and mixed dyes containing different types of dyes, and the phytotoxicity of decolorized dyes (single and mixed dyes) was significantly reduced. The crude laccase efficiently eliminated phytotoxicity associated with single and mixed dyes. Consequently, the crude laccase from Pleurotus eryngii offers significant potential for practical applications in the efficient decolorization and management of single and mixed dye pollutants with different chemical structures.
作者机构：
[Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Wu, Yang; Gong, Zhiyong] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Wu, Yang] W;[Yang, Xu] C;Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 9th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN)
会议时间：
AUG 17-20, 2018
会议地点：
Hohhot, PEOPLES R CHINA
会议主办单位：
[Li, Yan;Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Gong, Zhiyong;Wu, Yang] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.
关键词：
3D cell cuture;MnO2 nanowires;oxidative stress;vascular toxicity
期刊：
General and Comparative Endocrinology,2019年277:30-37 ISSN：0016-6480
通讯作者：
Zhao, Haobin
作者机构：
[Zhang, Runshuai; Wu, Kongyue; Shen, Hao; Al Hafiz, Abdullah; Xu, Gongyu; Wang, Zequn; Nibona, Emile; Ke, Xiaomei; Qi, Chao; Zhao, Haobin] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Zhao, Haobin] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.
会议名称：
1st International Symposium of Chinese Comparative Endocrinologists (ISOCCE) held jointly with 12th Academic Conference of Chinese-Society-for-Comparative-Endocrinology
会议时间：
JUN 30-JUL 03, 2018
会议地点：
Shanghai Ocean Univ, Shanghai, PEOPLES R CHINA
作者机构：
[Zhang, L.; Zhao, Y.; Magana, L. C.] Univ Calif Berkeley, Sch Publ Hlth, Berkeley, CA 94720 USA.;[Zhao, Y.] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
50th Annual Meeting of the Environmental-Mutagenesis-and-Genomics-Society (EMGS) on Environmental Mutagenesis and Genomics - The Next Fifty Years
作者机构：
[Li, Yan; Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Li, Jinquan; Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.;[Wang, Yixin; Gong, Zhiyong; Yang, Wu] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.;[Yang, Xu] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Yang, Wu] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构：
[Yang, Wu] W;[Yang, Xu] C;Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Hubei, Peoples R China.;Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 9th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN)
会议时间：
AUG 17-20, 2018
会议地点：
Hohhot, PEOPLES R CHINA
会议主办单位：
[Li, Yan;Wang, Pengcheng] Wuhan Polytech Univ, Key Lab Deep Proc Major Grain & Oil, Minist Educ, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.^[Li, Jinquan;Yang, Xu] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Coll Life Sci, Wuhan, Hubei, Peoples R China.^[Wang, Yixin;Gong, Zhiyong;Yang, Wu] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Coll Food Sci & Engn, Wuhan, Hubei, Peoples R China.
关键词：
3D organotypic culture;human lung fibroblasts;MnO2 nanowires;nanotoxicity
作者机构：
[Ma, Yingjun] Cent China Normal Univ, Sch Math & Stat, Wuhan 430079, Hubei, Peoples R China.;[Jiang, Xingpeng; He, Tingting; Zhang, Chenhao] Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.;[Jiang, Xingpeng; He, Tingting] Cent China Normal Univ, Hubei Prov Key Lab Artificial Intelligence & Smar, Wuhan 430079, Hubei, Peoples R China.;[Ge, Leixin] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构：
[Jiang, Xingpeng] C;Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Prov Key Lab Artificial Intelligence & Smar, Wuhan 430079, Hubei, Peoples R China.
会议名称：
IEEE International Conference on Bioinformatics and Biomedicine (BIBM) - Medical Genomics
会议时间：
DEC 03-06, 2018
会议地点：
Madrid, SPAIN
会议主办单位：
[Ma, Yingjun] Cent China Normal Univ, Sch Math & Stat, Wuhan 430079, Hubei, Peoples R China.^[He, Tingting;Zhang, Chenhao;Jiang, Xingpeng] Cent China Normal Univ, Sch Comp, Wuhan 430079, Hubei, Peoples R China.^[He, Tingting;Jiang, Xingpeng] Cent China Normal Univ, Hubei Prov Key Lab Artificial Intelligence & Smar, Wuhan 430079, Hubei, Peoples R China.^[Ge, Leixin] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
作者机构：
[Rao, Yan; Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.;[Yang, Xu; Ma, Ping] Hubei Univ Sci & Technol, Xianning, Peoples R China.;[Zhao, Wei; Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] H;[Yang, Xu] C;Hubei Univ Sci & Technol, Xianning, Peoples R China.;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 8th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN) / Symposium of Materials Science and Technology (SOMST)
会议时间：
OCT 13-16, 2017
会议地点：
Tianjin, PEOPLES R CHINA
会议主办单位：
[Huang, Xiaoxiao;Rao, Yan] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.^[Ma, Ping;Yang, Xu] Hubei Univ Sci & Technol, Xianning, Peoples R China.^[Zhao, Wei;Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
作者机构：
[Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.;[Zhao, Wei; Yang, Xu; Peng, Mei] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Zhao, Xiaoyan] Hubei Univ Med, Clin Coll 3, Shiyan, Hubei, Peoples R China.
通讯机构：
[Yang, Xu] C;Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.
会议名称：
Joint Symposium of the 8th International Conference of th Chinese-Society-of-Micro-Nano-Technology (CSMNT) / Microsystems and Nanoengineering Summit (MAN) / Symposium of Materials Science and Technology (SOMST)
会议时间：
OCT 13-16, 2017
会议地点：
Tianjin, PEOPLES R CHINA
会议主办单位：
[Huang, Xiaoxiao] Wuhan Sports Univ, Coll Art, Wuhan, Hubei, Peoples R China.^[Peng, Mei;Zhao, Wei;Yang, Xu] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.^[Zhao, Xiaoyan] Hubei Univ Med, Clin Coll 3, Shiyan, Hubei, Peoples R China.
关键词：
SWCNTs;nerve cells;protection effect;viability;vitamin C
期刊：
PROCEEDINGS 2018 IEEE INTERNATIONAL CONFERENCE ON BIOINFORMATICS AND BIOMEDICINE (BIBM),2018年:197-200 ISSN：2156-1125
通讯作者：
Hu, XH
作者机构：
[Ma, Yingjun] Cent China Normal Univ, Sch Math & Stat, Wuhan, Hubei, Peoples R China.;[Ge, Leixin] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Ma, Yuanyuan] Anyang Normal Univ, Sch Comp & Informat Engn, Anyang, Peoples R China.;[Jiang, Xingpeng; He, Tingting; Hu, Xiaohua; Hu, XH] Cent China Normal Univ, Sch Comp, Wuhan, Hubei, Peoples R China.
通讯机构：
[Hu, XH] C;Cent China Normal Univ, Sch Comp, Wuhan, Hubei, Peoples R China.
会议名称：
IEEE International Conference on Bioinformatics and Biomedicine (BIBM)
会议时间：
DEC 03-06, 2018
会议地点：
Madrid, SPAIN
会议主办单位：
[Ma, Yingjun] Cent China Normal Univ, Sch Math & Stat, Wuhan, Hubei, Peoples R China.^[Ge, Leixin] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.^[Ma, Yuanyuan] Anyang Normal Univ, Sch Comp & Informat Engn, Anyang, Peoples R China.^[Jiang, Xingpeng;He, Tingting;Hu, Xiaohua] Cent China Normal Univ, Sch Comp, Wuhan, Hubei, Peoples R China.
会议论文集名称：
IEEE International Conference on Bioinformatics and Biomedicine-BIBM
摘要：
Studies have shown that microRNAs are functionally related to human diseases. However, experimental methods for detecting miRNA-disease associations are both time consuming and laborious. Therefore, a large number of computational models for predicting potential miRNA-disease interaction have been proposed. However, few methods take into account the nonlinear structural similarity of miRNAs (diseases) and effectively integrate multiple similar metrics into one network. In this paper, we propose a kernel-based soft-neighborhood network propagation algorithm (LKSNF) to predict potential miRNA-disease interactions, which not only exploits the potential nonlinear relationship, but also effectively integrates different similar measures of miRNA (disease). The results of the 5-fold cross-validation show that the LKSNF model has significantly better predictive performance than other state-of-the-art methods. Case study further illustrates the effectiveness of LKSNF in predicting new miRNA-disease interactions.
作者机构：
[Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;[Qiu, Bao-Sheng; Zhang, Lu-Dan; Li, Zheng-Ke; Dai, Guo-Zheng; Yin, Yan-Chao; Zhang, Zhong-Chun] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
通讯机构：
[Qiu, Bao-Sheng] C;[Chen, Min] U;Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.;Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.;Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
会议名称：
Light Harvesting Satellite Meeting held in conjunction with the 17th International Congress on Photosynthesis Research
会议时间：
AUG, 2016
会议地点：
Egmond aan Zee, NETHERLANDS
会议主办单位：
[Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.^[Li, Zheng-Ke;Yin, Yan-Chao;Zhang, Lu-Dan;Zhang, Zhong-Chun;Dai, Guo-Zheng;Qiu, Bao-Sheng] Cent China Normal Univ, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Hubei, Peoples R China.^[Chen, Min] Univ Sydney, Sch Life & Environm Sci, Sydney, NSW 2006, Australia.
关键词：
Accessory chlorophyll-binding proteins (CBPs);Iron limitation;Iron-stress-induced protein A (IsiA);Photosynthesis;Six transmembrane helix family of chlorophyll-binding proteins/antenna;Acaryochloris marina
摘要：
The n-butyl benzyl phthalate (BBP) is an environmental pollutant used extensively in the manufacturing of plastics. For the bioremediation of phthalic acid ester pollutants in water, sediment, and soil, a BBP degrading bacterium Rhodococcus sp. HS-D2 was isolated from contaminated river sediment and characterized. The HS-D2 strain is capable of utilizing BBP as the sole source of carbon. A shaken culture containing 500 mgL(-1) of BBP produced complete degradation in 96 h. To study the metabolic characteristics and potential strategies for enhancing the biodegradation rates of BBP, a genome-scale metabolic model (GEM) of Rhodococcus sp. iYZ1601 was reconstructed based on the genome sequence of strain HS-D2. It included several sequential transporters and hydrolases were involved in the biodegradation process of BBP. Monoethylhexylphthalate (MEHP) and phthalic acid ester (PAE) hydrolases were confirmed as the key enzymes in phthalate degradation. The MEHP and PAE hydrolases catalyzed the conversion of BBP to butanal, phenylcarbinol, and phthalate as verified by HPLC analysis. The growth rate of HS-D2 and BBP consumption rate were analyzed in silico simulation, and were found to be consistent with the rates of HS-D2 growth and BBP consumption the in vitro experiment. In summary, Rhodococcus sp. HS-D2 biodegrades BBP, but the metabolic pathway of this bacterium needs further exploration to improve the biodegradation efficiency. (C) 2016 Elsevier Ltd. All rights reserved.
摘要：
In order to explore distant-site genotoxic effect of gaseous formaldehyde on genetic material of marrow cells in mice. SPF-class Kun Ming male mice were exposed to gaseous formaldehyde for 72 hours in such concentration as 0.5mg/m(3), 1.0mg/m(3) and 3.0mg/m(3). We applied the random amplified polymorphic DNA (RAPD) method to evaluate the genotoxic effect of the gaseous formaldehyde. The results of the RAPD profiles showed obvious differences between normal and exposed groups. The number of the varied bands increased gradually with the concentration of gaseous formaldehyde. Genomic template stability (GTS) analysis showed that with the increase of the gaseous formaldehyde concentration, the polymorphisms were50%, 39.66%, 32.76% and the GTS were 50%, 60.34%, 67.24% respectively. These results suggest that gaseous formaldehyde has significant genotoxicity and damage effect on the genetic material in marrow cells in this concentration range. Meanwhile, the DNA polymorphisms detected by RAPD technique could be used as a biomarker for detecting genetic effects of formaldehyde stress on mice.
摘要：
15 bacterial strains with a capacity of poly-phosphate were isolated from enrichment culture of the soil sample collected from the phosphate fertilizer factory of Honghu City in Hubei province, of which one strain named as HS-P7 has the strongest effects of poly-phosphate. Ammonium molybdate spectrophotometric method was used to measure its ability of poly-phosphate. The results showed that phosphorus removal rate about the strain HS-P7 reached to 90.08% when it was cultivated in the phosphorus-rich medium in 30℃ during 72 hours. The homology between the strain HS-P7 and Pseudomonas cf. Monteilii is 98% by comparing their 16S rDNA. Based on the characteristics of morphological, physiological and biochemical, the strain HS-P7 was identified as Pseudomonas sp. The effects of different temperatures, pH, carbon sources and nitrogen sources on the growth of the strain HS-P7 were investigated and the optimum growth condition was determined as pH 7.0 and 30℃.
会议论文集名称：
2010 4th International Conference on Bioinformatics and Biomedical Engineering. [v.4.1]
关键词：
Formaldehyde;Marrow cells;Cell cycle
摘要：
In order to explore the possibility of inhalation of formaldehyde on mouse bone marrow cells have an impact on cell cycle and use SPF level purely Kunming male mice for the study, with a concentration of 0.5 mg /m(3), 1.0 mg/m(3) and 3.0 mg/m(3) of gaseous Formaldehyde dynamics in mice exposed to continuous 72 hours after the exposure using flow cytometry analysis cells from mouse bone marrow cells, cell cycle changes. The results showed that: exposure group and control group the cell cycle there is an obvious difference between the data. These results suggest that: In the concentration range of formaldehyde on mouse bone marrow cells of the cell cycle changes in the role of clear.
作者机构：
[Yu, Tianhua] Jilin Univ, Sch Publ Hlth, Changchun, Jilin, Peoples R China.;[Yu, Tianhua] Jilin Univ, China Japanese Friendship Hosp, Dept Blood Transfus, Changchun, Jilin, Peoples R China.;[Xu, Chang] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.;[Zhang, Xinyu] Columbia Univ, Grad Sch Art & Sci Stat, New York, NY 10027 USA.
会议名称：
3rd AASRI Conference on Computational Intelligence and Bioinformatics (CIB)
会议时间：
NOV 25-26, 2015
会议地点：
Seoul, SOUTH KOREA
会议主办单位：
[Yu, Tianhua] Jilin Univ, Sch Publ Hlth, Changchun, Jilin, Peoples R China.^[Yu, Tianhua] Jilin Univ, China Japanese Friendship Hosp, Dept Blood Transfus, Changchun, Jilin, Peoples R China.^[Xu, Chang] Cent China Normal Univ, Sch Life Sci, Wuhan, Hubei, Peoples R China.^[Zhang, Xinyu] Columbia Univ, Grad Sch Art & Sci Stat, New York, NY 10027 USA.
摘要：
This paper addresses gene sequence data mining model by incorporating information theory and data dimension reduction algorithm. Unlike the previous work focusing more on the issues from the gene's perspective it-self, we combine the information science and data analysis model to achieve better performance. We firstly discuss the state-of-the-art reviews on the data dimension reduction algorithms to serve as the foundation. Later, we address the information theory into the traditional analysis model to synthetize to data analysis process. Then, we revise the basic data mining model with the assistance of the dimension reduction algorithm and information theory. Finally, we simulate the method and compare it with the other approaches and our algorithm out performs compared with the others.
作者机构：
[Yuan, Yongze; Yu, Jinhui; Xiong, Li; Liu, Deli; Li, Jinglong; Yu, Wen; Li, Dandan] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
会议名称：
International Conference on Biological Engineering and Biomedical (BEAB)
会议时间：
JAN 10-12, 2014
会议地点：
Yichang, PEOPLES R CHINA
会议主办单位：
[Li, Dandan;Yuan, Yongze;Yu, Jinhui;Yu, Wen;Li, Jinglong;Xiong, Li;Liu, Deli] Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Peoples R China.
摘要：
In this study, a bacterium strain, CZ-31, capable of degrading methyl parathion (MP) was isolated from active sludge of a wastewater treatment pond of a pesticide factory in Hubei province. According to morphological characteristics, physiological properties, and 16S rDNA sequence analysis, the isolates CZ-31 were identified as Arthrobacter ureafaciens. Methyl parathion degradation enzyme mpd gene was cloned by PCR from the genome of strain CZ-31. Nucleotide sequencing indicated that the mpd gene contains 996bp and is predicated to encode a peptide of 331 amino acids. The recombinant expression plasmid pET-mpd was constructed and successfully expressed in E. coli BL21 (DE3). The structure of methyl parathion degradation enzyme (MPD) was predicted by bioinformatics. Results showed MPD protein was 97.28% similar to MPH protein (from pseudomonas sp. WBC-3) and it belonged to metallo-beta-lactamases family. The model consists of 588 residues, three Zn2+, and one Cd2+. There is a homodimer comprising of two independent subunits in MPD structure. Each subunit is composed of alpha beta-lactamase-like domain, which includes the binuclear metal center. The binuclear metal site is located between the two beta-sheets and is surrounded by two alpha beta-loops. Homology modeling of MPD was constructed based on the crystal structure of MPH (PDBID:1P9E).
摘要：
In this experiment, the oxidative damage of nano-CdSeS in mice brains was performed. 20 male Kunming mice were divided into 4 groups and 3 experimental groups were exposed to different doses of nano-CdSeS (0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL) by intravenous administration while the control used saline solution instead. Three days later, the enzymatic activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the damage degree of DNA were determined to assess the oxidative damage in brain tissues. Our results showed that in the experimental groups, SOD activity was inhibited and MDA content was increased as the doses rising, at the same time, tail moment and tail DNA% increased significantly when comparing with the control. And these results exhibited a certain doses-dependency relations. From results above, it demonstrated that oxidative damage of brain induced by nano-CdSeS which enter into blood–brain barrier in mice.
