2-{4-[(E)-2,3-Diiodoallyl]-7-fluoro-3-oxo-3,4-dihydro-2H-benzo[b][1,4] oxazin-6-yl}-2,3,4,5,6,7-hexahydro-1H-isoindole-1,3-dione
作者:
Pang, Huai-Lin;Huang, Yin;Yin, Du-Lin* ;Yang, Hai;Li, Wen-An
期刊:
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY ,2007年63(5):o2742-o2742 ISSN:2053-2296
通讯作者:
Yin, Du-Lin
作者机构:
[Yin, Du-Lin] Hunan Normal Univ, Coll Chem & Chem Engn, Changsha, Hunan, Peoples R China.;Huazhong Normal Univ, Coll Chem, Wuhan, Hubei, Peoples R China.
通讯机构:
[Yin, Du-Lin] H;Hunan Normal Univ, Coll Chem & Chem Engn, Changsha, Hunan, Peoples R China.
摘要:
In the title compound, C19H15FI2N2O4, the cyclo-hexene and morpholinone rings adopt half-chair conformations. The dihedral angle between the benzene and pyrrole rings is 69.0 (2)°. The crystal packing is stabilized by C - H⋯O and C - H⋯F hydrogen bonds. © 2007 International Union of Crystallography. All rights reserved.
语种:
英文
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含***基的噻吩并嘧啶酮衍生物的合成与杀菌活性
作者:
孙勇;刘明国;丁明武
作者机构:
华中师范大学教育部农药与化学生物学重点实验室,武汉,430079
会议名称:
国家农药创制工程技术研究中心2007学术研讨会暨第四届湖南省湖北省农药植保学术交流会
会议时间:
2007-08-15
会议地点:
长沙
关键词:
噻吩并嘧啶酮衍生物;稠杂环化合物;合成工艺;杀菌活性;***基
摘要:
噻吩并嘧啶酮衍生物是一类具有良好生物活性的稠杂环化合物,如一些 2-烷氧基及烷基噻吩并嘧啶酮表现出优良的杀菌活性。另外,***类杀菌剂由于具有杀菌谱广、活性高、可内吸传导、价格合理等优点,一直占据了大量的杀菌剂市场。近年来,新型唑类杀菌剂的研究仍然十分活跃,并开发了一些性能更优良的杀菌剂品种。最近,氮杂 Wittig 反应广泛地应用于氮杂环的合成,该反应原料易得、条件温和、反应选择性好,已成为一种合成氮杂环的有效手段。笔者曾报道应用氮杂 Wittig 反应合成噻吩并嘧啶酮杂环的方法,部分化合物表现出抑菌活性,这里进一步报道应用该法合成含***基的噻吩并嘧啶酮杂环。
语种:
中文
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Rational design based on bioactive conformation analysis of pyrimidinylbenzoates as acetohydroxyacid synthase inhibitors by integrating molecular docking, CoMFA, CoMSIA, and DFT calculations
作者:
He, Yan-Zhen;Li, Yuan-Xiang;Zhu, Xiao-Lei;Xi, Zhen* ;Niu, Congwei;...
期刊:
Journal of Chemical Information and Modeling ,2007年47(6):2335-2344 ISSN:1549-9596
通讯作者:
Xi, Zhen
作者机构:
[Xi, Zhen] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China.
通讯机构:
[Xi, Zhen] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要:
Pyrimidinylthiobenzoates constitute an important kind of herbicides targeting acetohydroxyacid synthase (AHAS, EC 2.2.1.6), which catalyze the first common step in branched-chain amino acid biosynthesis. Due to the symmetry of 4,6-dimethoxypyrimidyl, there are two kinds of conformation of pyrimidinylthiobenzoates: one's phenyl is left-extending (named conformation-L);the other's phenyl is right-extending (named conformation-R). On the basis of the assumption that 3D quantitative structure-activity relationship (QSAR) models derived from the bioactive conformation should give the best result, a strategy of density-functional-theory-based 3D-QSAR was proposed to identify the bioactive conformation of pyrimidinylthiobenzoates by integrating the techniques of molecular docking, comparative molecular field analysis (CoMFA), comparative molecular similarity indices analysis (CoMSIA), and density functional theory calculation. The combination of three criteria of q <sup>2</sup>, r<sup>2</sup>, and r<inf>pred</inf><sup>2</sup> obtained from CoMFA and CoMSIA analyses clearly indicated that conformation-R rather than conformation-L might be the bioactive conformation for pyrimidinylthiobenzoates. A further comparison between the two binding modes indicated that pyrimidinylthiobenzoates and sulfonylureas have very similar binding sites, such as Trp586, Arg380, and Pro192. However, Lys251 formed H bonds with sulfonylureas rather than pyrimidinylthiobenzoates. In addition, the orientation of phenyl groups of the two classes of compounds in the binding pocket were revealed to be opposite, which explained why the mutation of Pro192 displayed different sensitivity to sulfonylureas and pyrimidinylthiobenzoates. On the basis of the understanding of interactions between pyrimidinyl-thiobenzoates and AHAS, we designed and synthesized six 8-(4,6-dimethoxypyrimidin-2-yloxy)-4- methylphthalazin-1-one derivatives according to the 3D-QSAR models. The excellent correlation between the tested K<inf>i</inf> values against wild-type A. thaliana acetohydroxyacid synthase and the predicted IC<inf>50</inf> values demonstrated the high reliability of the established 3D-QSAR models. To our knowledge, this is the first report highlighting the binding mode of herbicidal pyrimidinylthiobenzoates, which consisted of the reported results of herbicide resistance. ©2007 American Chemical Society.
语种:
英文
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蓖麻油聚氨酯互穿网络型聚合物性能的研究
作者:
石红娥;谭晓明;陈丽;李秀芳
期刊:
聚氨酯工业 ,2007年22(2):8-11 ISSN:1005-1902
作者机构:
华中师范大学化学学院,武汉,430079;孝感学院化学系,432100;[石红娥] 华中师范大学;[李秀芳; 陈丽; 谭晓明] 孝感学院
关键词:
互穿网络聚合物;蓖麻油;聚氨酯;性能
摘要:
分别用甲苯二异氰酸酯(TDI)和六亚甲基二异氰酸酯(HDI)与蓖麻油(CO)反应制备出两种聚氨酯预聚体,再用预聚体与苯乙烯、丙烯腈、甲基丙烯酸甲酯、环氧树脂(E-44或E-51)等单体制备出蓖麻油聚氨酯互穿网络型聚合物(CO-PU IPN),研究了体系组成对该聚合物拉伸强度的影响。结果表明,随着固化时间的延长,CO-PU IPN的拉伸强度逐渐增大,TDI型CO-PU IPN比HDI型的拉伸强度大;烯类单体比环氧树脂单体所制CO-PU IPN的拉伸强度大,不同烯类单体之间的差别不大;增加预聚体中NCO/OH的摩尔比,CO-PU IPN的拉伸强度都是先增加,后减小,在摩尔比为2.25时出现最大值;添加抗氧剂1010、紫外光吸收剂UV-327和光稳定剂292等对CO-PU IPN的拉伸强度基本没有影响。
语种:
中文
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抗痛风药物及其作用机制
作者:
黄露
期刊:
科协论坛(下半月) ,2007年(05):23+13 ISSN:1007-3973
作者机构:
华中师范大学化学学院,湖北·武汉,430079
关键词:
痛风病;尿酸;抗痛风药
摘要:
本文介绍了秋水仙碱、非甾体类抗炎药(NSAIDs)、促肾上腺皮质激素、糖皮质激素等抗痛风发作的有效药物及其作用机制。
语种:
中文
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Two salts of 5-sulfosalicylic acid and 3-amino-pyridine
作者:
Meng, Xiang-Gao* ;Zhou, Chun-Shan;Wang, Li;Liu, Chang-Lin
期刊:
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY ,2007年63(11):o667-o670 ISSN:2053-2296
通讯作者:
Meng, Xiang-Gao
作者机构:
[Meng, Xiang-Gao; Wang, Li; Zhou, Chun-Shan; Liu, Chang-Lin] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构:
[Meng, Xiang-Gao] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要:
5-Sulfosalicylic acid (5-SSA) and 3-amino-pyridine (3-APy) crystallize in the same solvent system, resulting in two kinds of 1:1 proton-transfer organic adduct, namely 3-aminopyridin-ium 3-carb-oxy-4-hydroxy-benzene-sulfonate monohydrate, C5H7N2 <sup>+</sup>·C7H5O6S<sup>-</sup>·H2O or 3-APy·5-SSA·H2O, (I), and the anhydrous adduct, C5H7N2 <sup>+</sup>·C7H5O6S<sup>-</sup> or 3-APy·5-SSA, (II). Both compounds have extensively hydrogen-bonded three-dimensional layered polymer structures, with inter-layer homo- and heterogeneous π-πinter-actions in (I) and (II), respectively. ©International Union of Crystallography 2007.
语种:
英文
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大学生手机消费状况的调查与分析
作者:
范重庆;向世莲;寇娜
期刊:
中国德育 ,2007年(05):41-43 ISSN:1673-3010
作者机构:
华中师范大学化学学院;华中师范大学化学学院 湖北武汉 430079
关键词:
大学生;“手机热”;消费心理;高校德育
摘要:
目前大学生拥有手机已很普遍。通过对大学生手机拥有状况、使用状况和消费心理的调查,指出大学校园"手机热"对大学生的学习和生活造成一定负面影响,必须引起高校德育工作者的充分重视,采取相应对策。
语种:
中文
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克氏原螯虾精巢与输精管组织细胞原代培养方法初探
作者:
刘凯于;姚汉超;邱宝国;郭建军;彭建新;...
期刊:
动物学杂志 ,2007年42(4):60-64 ISSN:0250-3263
作者机构:
华中师范大学生命科学学院,教育部农药与化学生物学重点实验室,武汉,430079;[郭建军; 姚汉超; 彭建新; 邱宝国; 洪华珠; 刘凯于] 华中师范大学
关键词:
克氏原螯虾;细胞培养;精巢;输精管;原代培养
摘要:
将剪碎的克氏原螯虾(Procambarus clarkii)精巢与输精管组织块分别接种于含20%FBS、双抗以及添加了适量无机盐等的M199-MK、MEM、S20、Grace's和L-15培养基中,25℃恒温静置培养.结果表明,在Grace's培养基中,只有少量组织块贴壁.在其余培养基中,大多数组织块能在1~2周内逐渐贴壁,L-15和S20培养基培养效果最好.组织块贴壁后,一些成纤维细胞和上皮样细胞逐渐从组织块迁移出来.传代后组织块可继续贴壁并重新迁移出一些成纤维细胞与少量上皮样细胞.添加部分草鱼细胞系PSF或斜纹夜蛾细胞系Sl驯化的培养基进行培养,驯化培养基∶新鲜培养基=1∶5(v/v),结果表明,Sl驯化培养基能促进细胞的迁移.组织块一般可离体培养2~4个月,但是都未能形成细胞单层.胰蛋白酶消化后分散的虾细胞不能贴壁.
语种:
中文
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Cationic corrole derivatives: a new family of G-quadruplex inducing and stabilizing ligands
作者:
Fu, Boqiao;Huang, Jing;Ren, Lige;Weng, Xiaocheng;Zhou, Yangyang;...
期刊:
Chemical Communications ,2007年(31):3264-3266 ISSN:1359-7345
通讯作者:
Zhou, Xiang
作者机构:
[Zhou, Xiang] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China.;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.
通讯机构:
[Zhou, Xiang] W;Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China.
摘要:
Water-soluble cationic corrole derivatives were designed and synthesized, and the first observation of their interactions with the telomeric G-quadruplex was made.
语种:
英文
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生物传感器的进展综述
作者:
李静
期刊:
科教文汇(下旬刊) ,2007年(08):204 ISSN:1672-7894
作者机构:
华中师范大学化学学院,湖北·武汉,430079
关键词:
生物传感器;原理;分类;前景
摘要:
生物传感器技术在医学领域中有广阔的应用前景,它具有专一、灵敏、响应快等特点,本文简要介绍了生物传感器的工作原理、分类,探讨了生物传感器的研究进展及应用前景。
语种:
中文
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Fe@Fe2O3 core-shell nanowires as the iron reagent. 2. An efficient and reusable sono-Fenton system working at neutral pH
作者:
Ai, Zhihui;Lu, Lirong;Li, Jinpo;Zhang, Lizhi* ;Qiu, Jianrong;...
期刊:
Journal of Physical Chemistry C ,2007年111(20):7430-7436 ISSN:1932-7447
通讯作者:
Zhang, Lizhi
作者机构:
[Zhang, Lizhi] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Huazhong Univ Sci & Technol, Natl Coal Combust Lab, Wuhan 430074, Peoples R China.;Xianning Coll, Dept Biol & Chem, Xianning 437100, Peoples R China.
通讯机构:
[Zhang, Lizhi] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要:
In this study, we developed an efficient and reusable sono-Fenton system with Fe@Fe2O3 core−shell nanowires as the iron reagent. Similar to our previous sono-Fenton system working at pH = 2, this system could also much more effectively degrade rhodamine B than those with Fe2+, Fe2+/Fe2O3, or commercial zerovalent iron particles as the iron reagents at neutral pH. The nature of the Fe@Fe2O3 nanowire Fenton iron reagent was examined by X-ray diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). It was found that this novel Fenton iron reagent could be recycled in this sono-Fenton system working at neutral pH. More importantly, the efficiency of this neutral-pH sono-Fenton system with adding 0.001 mol L-1 of Fe2+ even reached 92% for degrading RhB at neutral pH. This efficiency was close to that of our previous sono-Fenton system working at pH = 2. We proposed a possible mechanism for the sono-Fenton degradation of RhB over Fe@Fe2O3 core−shell nanowires at neutral pH, which involved an in situ recycling of iron species (Fe0 → Fen+ → Fe2O3). We believe that this economical and facile sono-Fenton system based on an Fe@Fe2O3 core−shell nanowire iron reagent may be applied to practical wastewater treatment.
语种:
英文
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糖杯芳烃基因载体GGC[4]的合成及性能研究
作者:
田德美;李海兵
期刊:
华中师范大学学报(自然科学版) ,2007年41(4):574-577 ISSN:1000-1190
作者机构:
[田德美; 李海兵] 华中师范大学化学学院
关键词:
糖杯芳烃;非病毒基因载体;凝胶电泳;透射电子显微镜
摘要:
设计并制备了一种新的具有分子探针功能的主体分子型非病毒载体双子糖杯芳烃主体分子(GGC[4]),应用透射电子显微镜(TEM)观测到主体分子GGC[4]与DNA分子相互作用形成超分子纳米复合物的微观结构形态.体外细胞转染实验表明其具有较高的转染效率.本研究为设计制备新的非病毒基因载体提供了一种新途径.
语种:
中文
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浅谈人教版化学必修教材中有关实验(实践)栏目的设置和实施
作者:
孙锡凤;汪阳
作者机构:
华中师范大学化学学院 湖北武汉 430079
会议名称:
全国化学教师教育第三届学术研讨会
会议时间:
2007-05-18
会议地点:
西安
会议论文集名称:
全国化学教师教育第三届学术研讨会论文集
关键词:
人教版;中学化学;必修教材;实验教学;课程标准
摘要:
人教版《普通高中课程标准实验教科书--化学(必修)》教材按照全面落实高中化学课程标准的要求,在设计上体现了"以学生发展为本,促进学生学习方式的转变,保证基础性,突出时代性,体现选择性,有利于学生科学探究活动的开展,反映科学、技术、社会的相互关系,结合高中学生的认知特点,重视学生的生活经验,注重学生的亲历性"的思路。
语种:
中文
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X-ray structures and binding properties of molecular clips based on diethoxycarbonyl glycoluril
作者:
Zhou, Bao H.;Cao, Li P.;Yin, Guo D.;Gao, M.;Wu, An X.*
期刊:
CANADIAN JOURNAL OF CHEMISTRY ,2007年85(9):586-591 ISSN:0008-4042
通讯作者:
Wu, An X.
作者机构:
[Cao, Li P.; Wu, An X.; Yin, Guo D.; Gao, M.; Zhou, Bao H.] Cent China Normal Univ, Dept Chem, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
通讯机构:
[Wu, An X.] C;Cent China Normal Univ, Dept Chem, Key Lab Pesticide & Chem Biol Minist Educ, Wuhan 430079, Peoples R China.
关键词:
crystal structure;molecular clips;binding properties
摘要:
The crystal structures of two molecular clips derived from diethoxycarbonyl glycoluril were reported. Their unique binding behavior towards hydroquinone, which is different from Nolte's clips (high affinity for resorcinol), has been characterized through 1H NMR and IR. Job-plot analyses provide good evidence of a 1:1 stoichiometry for the complexes. © 2007 NRC Canada.
语种:
英文
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N-(7-氟-3-氧-3,4-二氢-2H-苯并[b][1,4]噁嗪-6-基-)-N’,N’-二取代脲类化合物的合成及除草活性
作者:
李宏伟;任叶果;陈正旺;闵忠诚;胡礼;...
期刊:
精细化工中间体 ,2007年37(6):22-25 ISSN:1009-9212
作者机构:
华中师范大学化学学院;湖南化工研究院国家农药创制工程技术研究中心;湖南师范大学化学化工学院;[黄明智; 胡礼; 任叶果] 湖南化工研究院;[李宏伟; 闵忠诚] 华中师范大学
关键词:
除草剂;苯并[b][1;4]噁嗪;乙酰胺;生物活性
摘要:
设计并合成了9个N-(7-氟-3-氧-3,4-二氢-2H-苯并[b][1,4]噁嗪-6-基-)-N′,N′-二取代脲类化合物,其化学结构经IR、1HNMR、LC/MS确证.初步生物活性测定结果表明,部分化合物具有一定的除草活性,当茎叶处理使用剂量为75g a.i./hm2时,化合物(4)e和(4)h对双子叶杂草刺苋的抑制率达70%以上.
语种:
中文
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Synthesis, Crystal Structure and Bioactivities of 1-(4-Chlorophenyl)-3-[5-(pyrid-4-yl)- 1,3,4-thiadiazol-2-yl]urea
作者:
Song Xin-Jian* ;Liu Xin-Ping;Tan Xiao-Hong;Wang Yan-Gang
期刊:
Chinese Journal of Structural Chemistry ,2007年26(4):467-471 ISSN:0254-5861
通讯作者:
Song Xin-Jian
作者机构:
[Song Xin-Jian] Hubei Inst Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Enshi 445000, Peoples R China.;Hubei Inst Nationalities, Sch Chem & Environm Engn, Enshi 445000, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构:
[Song Xin-Jian] H;Hubei Inst Nationalities, Key Lab Biol Resources Protect & Utilizat Hubei P, Enshi 445000, Peoples R China.
关键词:
1,3,4-thiadiazole;urea;synthesis;crystal structure;bioactivities
摘要:
The title compound 1-(4-chlorophenyl)-3-[5-(pyrid-4-yl)-1,3,4-thiadiazol-2-yl]urea (C14H10ClN5OS, Mr = 331.79) has been synthesized by the reaction of 2-amino-5-(pyrid-4-yl)- 1,3,4-thiadiazole with 4-chlorobenzoyl azide, and its crystal structure was determined by single-crystal X-ray diffraction. The crystal belongs to triclinic system, space group P with a = 5.8550(8), b = 7.5668(10), c = 16.416(2) , α = 78.364(2), β = 81.204(2), γ = 84.749(2)°, V = 702.58(16) 3, Z = 2, Dc = 1.568 g/cm3, μ = 0.429 mm-1, F(000) = 340, the final R = 0.0442 and wR = 0.1092 for 2001 observed reflections (I > 2σ(I)). X-ray diffraction analysis reveals that the title molecule is nearly planar. In the crystal structure, the molecules are linked by strong intermolecular N-H…N hydrogen bonds together with weak nonclassical intermolecular (C-H…Y, Y = N, O and Cl) hydrogen bonds and stacked through π-π interactions. The preliminary bioassay shows that the title compound exhibits good fungicidal activities against Rhizoctonia solani, Botrytis cinerea and Dothiorella gregaria.
语种:
英文
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Two new bromo-functionalized organoimido derivatives of hexamolybdate: Synthesis, crystal structure, spectroscopic and electrochemical studies
作者:
Li, Qiang;Zhu, Li;Meng, Xianggao;Zhu, Yulin;Hao, Jian;...
期刊:
Inorganica Chimica Acta ,2007年360(8):2558-2564 ISSN:0020-1693
通讯作者:
Wei, Yongge
作者机构:
[Wei, Yongge] Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China.;Chengde Petr Coll, Dept Chem Engn, Hebei 067000, Peoples R China.;Hunan Univ Sci & Technol, Dept Chem & Chem Engn, Xiangtan 411201, Peoples R China.;Cent China Normal Univ, Dept Chem, Wuhan 430079, Peoples R China.
通讯机构:
[Wei, Yongge] T;Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China.
关键词:
Aromatic amine halides;Crystal structures;DCC;Organic derivatives;Organic-inorganic hybrids;Polyoxometalates
摘要:
Two organic-inorganic hybrid compounds, (Bu4N)2[Mo6O18(NAr)] (Ar = 2-CH3-4-BrC6H3 (1) or 2,6-CH3-4-BrC6H2 (2)) have been synthesized via the DCC dehydrating protocol of the reaction of [α-Mo8O26]4- with 2-methyl-4-bromoaniline hydrochloride or 2,6-dimethyl-4-bromoaniline hydrochloride in anhydrous acetonitrile, which have been characterized by UV-Vis spectra, 1H NMR, IR, cyclic voltammetry and X-ray single-crystal diffraction study. Both compounds crystallize in the monoclinic space group P over(1, ̄), which are featured in a terminal phenylimido group linked to a Mo atom of a hexamolybdate cluster by a Mo-N triple bond. Interestingly, there are two conformational isomers of the cluster anions of 1 and 2 in the crystals. By cyclic voltammetry study, their special redox properties were also found in the end. © 2007 Elsevier B.V. All rights reserved.
语种:
英文
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4-[4-(Benzyloxy)phenyl]-1,1,1-trifluoro-4-hydroxybut-3-en-2-one
作者:
Wang, Dun-Jia* ;Fan, Ling;Zheng, Jing
期刊:
ACTA CRYSTALLOGRAPHICA SECTION C-STRUCTURAL CHEMISTRY ,2007年63(6):o2979-o2979 ISSN:2053-2296
通讯作者:
Wang, Dun-Jia
作者机构:
[Wang, Dun-Jia] Hubei Normal Univ, Dept Chem & Environm Engn, Hubei Key Lab Bioanalyt Tech, Huangshi 435002, Peoples R China.;Cent China Normal Univ, Coll Chem, Wuhan 430079, Peoples R China.
通讯机构:
[Wang, Dun-Jia] H;Hubei Normal Univ, Dept Chem & Environm Engn, Hubei Key Lab Bioanalyt Tech, Huangshi 435002, Peoples R China.
摘要:
The mol-ecule of the title compound, C17H13F3O3, exists in the enol form and displays a strong intra-molecular O - H⋯O hydrogen bond. The trifluoro-methyl group is disordered over two orientations in an approximate 2:1 ratio. © International Union of Crystallography 2007.
语种:
英文
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An amperometric acetylthiocholine sensor based on immobilization of acetylcholinesterase on a multiwall carbon nanotube-cross-linked chitosan composite
作者:
Du, Dan
* ;Huang, Xi;Cai, Jie;Zhang, Aidong
( 张爱东 ) ;Ding, Jiawang;...
期刊:
Analytical and Bioanalytical Chemistry ,2007年387(3):1059-1065 ISSN:1618-2642
通讯作者:
Du, Dan
作者机构:
[Du, Dan] Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Technol Ctr Wuhan Iron & Steel Co, Wuhan 430080, Peoples R China.
通讯机构:
[Du, Dan] C;Cent China Normal Univ, Minst Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
关键词:
Acetylcholinesterase;Acetylthiocholine sensor;Chitosan;Composite film;Multiwall carbon nanotube
摘要:
A simple method has been devised for immobilization of acetylcholinesterase (AChE)-covalent bonding to a multiwall carbon nanotube (MWNT)-cross-linked chitosan composite (CMC)-and a sensitive amperometric sensor for rapid detection of acetylthiocholine (ATCl) has been based on this. Fourier-transform infrared spectroscopy proved that the native structure of the immobilized enzyme was preserved on this chemically clean and homogeneous composite film, because of the excellent biocompatibility and non-toxicity of chitosan. Glutaraldehyde was used as cross-linker to covalently bond the AChE, and efficiently prevented leakage of the enzyme from the film. Because of the inherent conductive properties of the MWNT, the immobilized AChE had greater affinity for ATCl and excellent catalytic effect in the hydrolysis of ATCl, with a $$K^{{{\text{app}}}}_{{\text{m}}} $$ value of 132 μmol L-1, forming thiocholine, which was then oxidized to produce a detectable and rapid response. Under optimum conditions the amperometric current increased linearly with the increasing concentration of ATCl in the range 2.0-400 μmol L-1, with a detection limit of 0.10 μmol L-1. Fabrication reproducibility of the sensor was good and the stability was acceptable. The sensor is a promising new tool for characterization of enzyme inhibitors and for pesticide analysis. [Figure not available: see fulltext.]. © Springer-Verlag 2007.
语种:
英文
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木屑纤维素酶水解条件的试验研究
作者:
栗薇;吴正舜
期刊:
可再生能源 ,2007年25(2):41-43 ISSN:1671-5292
作者机构:
华中师范大学,化学学院,湖北,武汉,430079;[栗薇; 吴正舜] 华中师范大学
关键词:
木屑;预处理;酶水解
摘要:
采用正交试验法研究了稀盐酸预处理木屑的最优条件:反应温度为105℃,反应时间为3 h,用质量分数为2%的HCl预处理后,半纤维素质量分数降低了78.4%,木质素降低了29.3%。用纤维素酶水解预处理过的木屑,考察了pH值、温度、时间对酶水解率的影响,结果表明:酶解温度为50℃,pH值为4.8,纤维素酶液用量为2 ml/g,水解时间为48 h时,酶水解率达到76%,纤维素质量分数降低了65.4%。
语种:
中文
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