期刊:
Journal of Thermoplastic Composite Materials,2023年36(11):4349-4364 ISSN:0892-7057
作者机构:
[Wu, Qiangxian; Zhang, Peirui; Jian, Kui] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan, Peoples R China.;[Weng, Fangqing] Hubei Univ Educ, Coll Chem & Life Sci, Wuhan, Peoples R China.;[Zhuang, Kejia; Hong, Liu; Gao, Shanjun; Zhao, Weiwei] Wuhan Univ Technol, Sch Mat Sci & Engn, Wuhan, Peoples R China.;[Wu, Qiangxian] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Luoyu Rd 152, Wuhan 430079, Hubei, Peoples R China.
关键词:
shell;polycaprolactone;composite;CaCO3
摘要:
Journal of Thermoplastic Composite Materials, Volume 36, Issue 11, Page 4349-4364, November 2023. <br/>Agricultural waste shells are another source of calcium carbonate (CaCO3). Waste shells contain natural polymers such as polysaccharides and protein which can react with active groups in a polymer by melt mixing to form a compatible layer in a composite material. The formation of a compatible layer helps to improve the compatibility and material toughness of a composite. At the same time, the presence of CaCO3 in waste shells can also help to improve the hardness of a material. Therefore, this work mainly focused on the preparation of composite materials using different kinds of shell powder, and also studied the effects of the interface structure between shell powder and polycaprolactone (PCL) and properties of the composite materials. In particular, this work, studied the effects of the crystal forms of various shell powder as well as the content of polysaccharide and protein on the tensile performance and thermal properties of composite materials. The results implied that the tensile performance of the composites prepared from different kinds of shell powder were slightly different. The composite prepared from shell powder containing aragonite-structured CaCO3 had a better elastic modulus than the composite prepared from shell powder containing calcite-structured CaCO3. Likewise, the composite material prepared from scallop shell powder had the best toughness and thermal properties. This work has laid a foundation for exploring the effects of all sorts of shell powder on the structure and properties of composite material.
作者机构:
[Tang, Yuchen; Gao, Tingjuan; Zheng, Xingxing] Cent China Normal Univ, Coll Chem, China Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构:
[Tang, YC; Gao, TJ ] C;Cent China Normal Univ, Coll Chem, China Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
摘要:
High-throughput assays play an important role in the fields of drug discovery, genetic analysis, and clinical diagnostics. Although super-capacity coding strategies may facilitate labeling and detecting large numbers of targets in a single assay, practically, the constructed large-capacity codes have to be decoded with complicated procedures or are lack of survivability under the required reaction conditions. This challenge results in either inaccurate or insufficient decoding outputs. Here, we identified chemical-resistant Raman compounds to build a combinatorial coding system for the high-throughput screening of cell-targeting ligands from a focused 8-mer cyclic peptide library. The accurate in situ decoding results proved the signal, synthetic, and functional orthogonality for this Raman coding strategy. The orthogonal Raman codes allowed for a rapid identification of 63 positive hits at one time, evidencing a high-throughput-out capability in the screening process. We anticipate this orthogonal Raman coding strategy being generalized to enable efficient high-throughput-out screening of more useful ligands for cell targeting and drug discovery.
摘要:
A compact 4-port coplanar waveguide (CPW) fed ultra-wideband (UWB) multiple-input-multiple-output (MIMO) antenna with enhanced isolation is proposed to operate from 3.66 GHz to 16.61 GHz with 127.78% impedance bandwidth. The proposed antenna system consists of 4 orthogonal arrays of monopole antennas improved by four iterations. The UWB characteristic of the antenna is obtained from an improved monopole antenna fed by CPW. The final MIMO antenna is machined on a Rogers 4003C substrate with dimensions of 60 x 60 x 0.3 mm(3). The orthogonal arrangement of antennas brings about the polarization diversity and certain isolation of MIMO antennas. Four rows of orthogonal metasurface elements with via holes and the ring of the backplane further improve the isolation between antenna elements in the MIMO system. The MIMO antenna has an impedance bandwidth of 12.95 GHz with S-11 < -10 dB and good isolation (S-21 =S-41 < -30 dB and S-31 < -20 dB). In particular, S31 is always less than -25 dB at frequencies greater than 6 GHz. The measured results of MIMO antenna agree with the simulated results. The optimized MIMO antenna has excellent performance in many aspects, such as UWB, compact size, omnidirectional radiation, enhanced isolation and high efficiency, which means that it has application potential in UWB wireless communication systems.
作者机构:
[Feng, Guoqiang; Li, Qianhua; Gong, Shengyi; Jiang, Siyu; Zhu, Wenlong] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构:
[Guoqiang Feng] K;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan 430079, China
作者机构:
[Bao, Guang-Ming; Wang, Yongze; Luo, Xin; Li, Shentian] Hubei Univ Technol, Hubei Prov Cooperat Innovat Ctr Ind Fermentat, Natl Ctr Cellular Regulat & Mol 111, Hubei Res Ctr Food Fermentat Engn,Key Lab Fermenta, Wuhan 430068, Hubei, Peoples R China.;[Xu, Weiqing; Luo, Zhen; Fang, Qie; Zhu, Chengzhou; Gu, Wenling; Wang, Hengjia] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.;[Zhu, CZ] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
通讯机构:
[Zhu, CZ ] ;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
期刊:
The Journal of Organic Chemistry,2023年88(11):7463-7468 ISSN:0022-3263
通讯作者:
Ke Gao
作者机构:
[Gao, Ke; Sun, Yulin] Cent China Normal Univ, CCNU uOttawa Joint Res Ctr, Key Lab Pesticide & Chem Biol, Minist Educ,Coll Chem, Wuhan 430079, Hubei, Peoples R China.
通讯机构:
[Ke Gao] C;CCNU-uOttawa Joint Research Centre, Key Laboratory of Pesticide & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei 430079, P. R. China
摘要:
An inexpensive and efficient aminoguanidine-catalyzed reductive cyclization of o-phenylenediamines with CO2 in the presence of triethoxysilane is described. Various functionalized benzimidazoles, benzoxazole, and benzothiazole were synthesized in high yields. Mechanistic studies indicate that formic acid as a cocatalyst promotes the cyclization reaction.
作者:
Gao, Yang-Yang;Yang, Wei-Cheng;Ashby Jr, Charles R.;Hao, Ge-Fei
期刊:
Drug Resistance Updates,2023年67:100934 ISSN:1368-7646
作者机构:
[Yang, Wei-Cheng; Gao, Yang-Yang; Hao, Ge-Fei] Guizhou Univ, Ctr Res & Dev Fine Chem, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn,Minist Educ, Guiyang 550025, Peoples R China.;[Hao, Ge-Fei] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Ashby Jr, Charles R.] St Johns Univ, Dept Pharmaceut Sci, New York, NY 10003 USA.
关键词:
Drug resistance;Cryptic binding sites;Resistance management
摘要:
The emergence of drug resistance is a primary obstacle for successful chemotherapy. Drugs that target cryptic binding sites (CBSs) represent a novel strategy for overcoming drug resistance. In this short communication, we explain and discuss how the discovery of CBSs and their inhibitors can overcome drug resistance.
作者:
Li Li;Xiaoxie Ma;Yusong Peng;Jun Yin;Nida El Islem Guissi;...
期刊:
ACS Applied Bio Materials,2023年6(4):1639-1649 ISSN:2576-6422
通讯作者:
Jun Yin<&wdkj&>Nida El Islem Guissi<&wdkj&>Yiqing Wang
作者机构:
[Li Li; Yusong Peng; Nida El Islem Guissi; Yiqing Wang] Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, People’s Republic of China;[Xiaoxie Ma; Jun Yin] Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People’s Republic of China
通讯机构:
[Jun Yin] K;[Nida El Islem Guissi; Yiqing Wang] D;Department of Biomedical Engineering, College of Engineering and Applied Sciences, Nanjing University, Nanjing 210023, People’s Republic of China<&wdkj&>Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, People’s Republic of China
摘要:
Fluorescent probes with fluorescence emission in the NIR-II window have been widely studied due to increased imaging depth. However, the currently reported NIR-II fluorescent probes present some disadvantages, such as complicated synthesis routes and low fluorescence quantum yields (QYs). The shielding strategy has been used in the development of NIR-II probes to improve their QYs. So far, this strategy has only been used for the symmetric NIR-II probes, especially those based on the benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole) (BBTD) skeleton. This work reports the synthesis of a series of asymmetric NIR-II probes based on shielding strategies accompanied by simple synthetic routes, high synthetic yields (above 90%), high QYs, and large Stoke shifts. Furthermore, the use of d-α-tocopheryl polyethylene glycol succinate (TPGS) as a surfactant for an NIR-II fluorescence probe (NT-4) improved its water solubility. In vivo studies showed that TPGS-NT-4 NPs with a high QY (3.46%) achieve high-resolution angiography and efficient local photothermal therapy, while displaying good biocompatibility. Hence, we combined angiography and local photothermal therapy to improve the tumor uptake of nanophotothermal agents while reducing their damage to normal tissues.
作者机构:
[Chen, Yuanxing; Xu, Wentao; Wen, Jing; Peng, Xiang; Hu, Liuyong; Xiao, Runshi] Wuhan Inst Technol, Hubei Engn Technol Res Ctr Optoelect & New Energy, Hubei Key Lab Plasma Chem & Adv Mat, Wuhan 430205, Peoples R China.;[Qin, Ying; Zhu, Chengzhou; Chen, Yuanxing; Gu, Wenling; Liu, Mingwang; Gu, WL; Xiao, Runshi; Tan, Rong] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.;[Yu, Han] Hong Kong Univ Sci & Technol, Energy Inst, Guangdong Hong Kong Macao Joint Lab Optoelect & Ma, Dept Chem,Kowloon, Clear Water Bay, Hong Kong 999077, Peoples R China.;[Yu, Han] Hong Kong Univ Sci & Technol, Hong Kong Branch, Chinese Natl Engn Res Ctr Tissue Restorat & Recons, Kowloon, Clear Water Bay, Hong Kong 999077, Peoples R China.
通讯机构:
[Hu, LY ] W;[Gu, WL ] C;Wuhan Inst Technol, Hubei Engn Technol Res Ctr Optoelect & New Energy, Hubei Key Lab Plasma Chem & Adv Mat, Wuhan 430205, Peoples R China.;Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.
摘要:
The intelligent molecular design of organic semiconductors allows for the introduction of specific moieties with recognition functions, while the recognition reactions are largely overlooked for constructing an advanced photoelectrochemical sensor. For the first time, a photoelectrochemical sensing platform for urease detection based on organic semiconductor heterojunction (PM6‐Y6) is constructed benefiting from the selective chemical reactivity of its organic building blocks. Abstract Non‐fullerene acceptors (NFAs) are a crucial component of organic photovoltaics, and they have gained significant attention due to their outstanding photoelectric conversion efficiency. However, the recognition reactions of specific building blocks in NFAs are largely overlooked in the construction of photoelectrochemical (PEC) biosensing platforms. In this study, the potential of Y6, a prototype NFA, is explored to construct a sensitive PEC biosensor for monitoring urease activity due to the selective chemical reactivity of its organic building blocks. The resultant biosensor relies on the urease‐mediated enzymatic reaction, which produces OH− anions that act as a nucleophilic reagent for the linkage of C═C in the Y6 moiety. This results in the formation of Y6‐OH, which exhibits a depressive photoelectric response due to the destroyed conjugated structure and intramolecular charge transfer. As expected, a linear relationship is observed between the recession of photoelectric performance and the concentration of urease, with good sensitivity and selectivity. Furthermore, urease activity detection is also successfully realized in human saliva samples, suggesting the promising potential of NFA‐based PEC biosensors for clinical applications even in the absence of common biological recognition units.
通讯作者:
Prof. Jong Seung Kim<&wdkj&>Prof. Yao Sun<&wdkj&>Prof. Jong Seung Kim Prof. Jong Seung Kim Prof. Jong Seung Kim<&wdkj&>Prof. Yao Sun Prof. Yao Sun Prof. Yao Sun
作者机构:
[Le Tu; Chonglu Li; Longcan Mei; Prof. Junrong Li; Prof. Yao Sun; Le Tu Le Tu Le Tu; Chonglu Li Chonglu Li Chonglu Li; Longcan Mei Longcan Mei Longcan Mei; Prof. Junrong Li Prof. Junrong Li Prof. Junrong Li; Prof. Yao Sun Prof. Yao Sun Prof. Yao Sun] National Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, 430079 China;[Prof. Xiaoxing Xiong; Prof. Xiaoxing Xiong Prof. Xiaoxing Xiong Prof. Xiaoxing Xiong] Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060 China;[Ji Hyeon Kim; Prof. Jong Seung Kim; Ji Hyeon Kim Ji Hyeon Kim Ji Hyeon Kim; Prof. Jong Seung Kim Prof. Jong Seung Kim Prof. Jong Seung Kim] Department of Chemistry, Korea University, Seoul, 02841 Korea;[Qian Li; Qian Li Qian Li Qian Li] Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Qingdao University of Science & Technology, Qingdao, 266100 China;[Prof. Shuang Liu; Prof. Shuang Liu Prof. Shuang Liu Prof. Shuang Liu] School of Materials Science and Engineering, Wuhan University of Technology, Wuhan, 430070 China
通讯机构:
[Prof. Jong Seung Kim; Prof. Jong Seung Kim Prof. Jong Seung Kim Prof. Jong Seung Kim] D;[Prof. Yao Sun; Prof. Yao Sun Prof. Yao Sun Prof. Yao Sun] N;National Key Laboratory of Green Pesticide, College of Chemistry, Central China Normal University, Wuhan, 430079 China<&wdkj&>Department of Chemistry, Korea University, Seoul, 02841 Korea
摘要:
Although metallacycle-based supramolecular photosensitizers (PSs) have attracted increasing attention in biomedicine, their clinical translation is still hindered by their inherent dark toxicity. Herein, we report what to our knowledge is the first example of a molecular engineering approach to building blocks of metallacycles for constructing a series of supramolecular PSs (RuA–RuD), with the aim of simultaneously reducing dark toxicity and enhancing phototoxicity, and consequently obtaining high phototoxicity indexes (PI). Detailed in vitro investigations demonstrate that RuA–RuD display high cancer cellular uptake and remarkable antitumor activity even under hypoxic conditions. Notably, RuD exhibited no dark toxicity and displayed the highest PI value (≈406). Theoretical calculations verified that RuD has the largest steric hindrance and the lowest singlet-triplet energy gap (ΔEST, 0.61 eV). Further in vivo studies confirmed that RuD allows safe and effective phototherapy against A549 tumors.
通讯机构:
[Ye, F ; Bao, F] C;[Wu, CG ] M;Cent China Normal Univ, CCNU uOttawa Joint Res Ctr, Key Lab Pesticide & Chem Biol, Minist Educ,Coll Chem, Wuhan 430079, Hubei, Peoples R China.;Minist Educ, Key Lab Xinan Med, Hefei 230038, Anhui, Peoples R China.;Wuhan Inst Photochem & Technol, Wuhan 430082, Hubei, Peoples R China.
摘要:
We report here a general alkylation reaction of terminal alkenes with nucleophilic cobaloxime complex catalysis under visible light irradiation. A broad range of vinyl arenes and heteroarenes, as well as a diverse set of alkyl(pseudo)halides or strained rings as alkyl electrophiles, can efficiently undergo the transformation up to the gram scale. Mechanistic study reveals the radical nature of the reaction and corroborates our design involving alkylcobaloxime as the key intermediate.
期刊:
ACS Chemical Biology,2023年18(4):915-923 ISSN:1554-8929
通讯作者:
Jun Guo
作者机构:
[Zhang, Zhi-Ming; Zhang, Ru-Yan; Feng, Ran-Ran; Ding, Dong; Wen, Yu; Guo, Jun; Zhou, Shi-Hao] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Hubei Int Sci & Technol Cooperat Base Pesticide &, Wuhan 430079, Peoples R China.;[Wei, Hua-Wei] Jiangsu East Mab Biomed Technol Co Ltd, Nantong 226499, Peoples R China.
通讯机构:
[Jun Guo] K;Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, College of Chemistry, Central China Normal University, Wuhan 430079, China
摘要:
Modification of antigens to improve their immunogenicity represents a promising direction for the development of protein vaccine. Here, we designed facilely prepared adjuvant-free vaccines in which the N-glycan of SARS-CoV-2 receptor-binding domain (RBD) glycoprotein was oxidized by sodium periodate. This strategy only minimally modifies the glycans and does not interfere with the epitope peptides. The RBD glycoprotein oxidized by high concentrations of periodate (RBD(HO)) significantly enhanced antigen uptake mediated by scavenger receptors and promoted the activation of antigen-presenting cells. Without any external adjuvant, two doses of RBD(HO) elicited 324- and 27-fold increases in IgG antibody titers and neutralizing antibody titers, respectively, compared to the unmodified RBD antigen. Meanwhile, the RBD(HO) vaccine could cross-neutralize all of the SARS-CoV-2 variants of concern. In addition, RBD(HO) effectively enhanced cellular immune responses. This study provides a new insight for the development of adjuvant-free protein vaccines.
摘要:
Catalytic oxidation technology is currently considered as a feasible approach to degrade and mineralize volatile organic compounds (VOCs). However, it is still challenging to realize efficient removal of VOCs through catalytic oxidation at room temperature. In our study, a novel flow-through electrocatalytic reactor was designed, composed of porous solid-electrolyte, gas-permeable titanium sub-oxides/titanium-foam (TiSO/Ti-foam) as anode and platinum coated titanium foam (Pt/Ti-foam) as cathode. This device could oxidize nearly 100% of benzene (10 ppm) to carbon dioxide at a current density of 1.2 mA/cm2 under room temperature. More importantly, the device maintained excellent stability over 1000 h. Mechanism of benzene mineralization was discussed. Hydroxyl radicals generated on the TiSO/Ti-foam anode played a crucial role in the oxidation of benzene. This study provides a promising prototype of the electrochemical air purifier, and may find its application in domestic and industrial air pollution control.
摘要:
The immune checkpoint blockade (ICB) antibody immunotherapy has demonstrated clinical benefits for multiple cancers. However, the efficacy of immunotherapy in tumors is suppressed by deficient tumor immunogenicity and immunosuppressive tumor microenvironments. Pyroptosis, a form of programmed cell death, can release tumor antigens, activate effective tumor immunogenicity, and improve the efficiency of ICB, but efficient pyroptosis for tumor treatment is currently limited. Herein, we show a mild hyperthermia-enhanced pyroptosis-mediated immunotherapy based on hollow carbon nanozyme, which can specifically amplify oxidative stress-triggered pyroptosis and synchronously magnify pyroptosis-mediated anticancer responses in the tumor microenvironment. The hollow carbon sphere modified with iron and copper atoms (HCS-FeCu) with multiple enzyme-mimicking activities has been engineered to induce cell pyroptosis via the radical oxygen species (ROS)-Tom20-Bax-Caspase 3-gasdermin E (GSDME) signaling pathway under light activation. Both in vitro and in vivo antineoplastic results confirm the superiority of HCS-FeCu nanozyme-induced pyroptosis. Moreover, the mild photothermal-activated pyroptosis combining anti-PD-1 can enhance antitumor immunotherapy. Theoretical calculations further indicate that the mild photothermal stimulation generates high-energy electrons and enhances the interaction between the HCS-FeCu surface and adsorbed oxygen, facilitating molecular oxygen activation, which improves the ROS production efficiency. This work presents an approach that effectively transforms immunologically "cold" tumors into "hot" ones, with significant implications for clinical immunotherapy.
作者机构:
[Gao, Ke; Zhang, He; Zhang, Yanmeng] Cent China Normal Univ, Coll Chem, CCNU uOttawa Joint Res Ctr, Key Lab Pesticides & Chem Biol,Minist Educ, 152 Luoyu Rd, Wuhan, Hubei, Peoples R China.
通讯机构:
[Ke Gao] C;CCNU-uOttawa Joint Research Centre, Key Laboratory of Pesticides & Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei, P.R. China
摘要:
The selective reductive N-methylation and N,N-dimethylation of primary amines using CO2 are some of the most significant challenges faced by organic chemists. Herein, we report the highly selective N,N′-diisopropylcarbodiimide-catalyzed methylation of primary amines using 1 atm CO2 under metal-free conditions. Borane–piperazine and borane–trimethylamine complexes were used as reducing agents for the N-methylation and N,N-dimethylation of various aromatic primary amines, respectively, in the presence of CO2. Mechanistic studies suggest that the selectivity of methylation is controlled by the steric effects of amines and boranes.
期刊:
Journal of Materials Science,2023年58(31):12764-12774 ISSN:0022-2461
通讯作者:
Cao, Y;Chen, DD
作者机构:
[Cao, Y; Asibaike, Laizaiti; Mukerabigwi, Jean Felix; Wu, Xuelin; Cao, Yu; Qu, Jiaying; Sun, Yuyang; Huang, Xueying] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Chen, Didi; Qu, Jiaying] Hubei Univ Educ, Hubei Key Lab Purificat & Applicat Plant Anticanc, Wuhan 430205, Peoples R China.;[Mukerabigwi, Jean Felix] Univ Rwanda, Coll Sci & Technol, Dept Chem, POB 3900, Kigali, Rwanda.
通讯机构:
[Cao, Y ] C;[Chen, DD ] H;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Hubei Univ Educ, Hubei Key Lab Purificat & Applicat Plant Anticanc, Wuhan 430205, Peoples R China.
摘要:
Due to its small particle size, high specific surface area, functional capability and long-term stable fluorescence, nanodiamond (ND) has been widely used in biomedical applications including fluorescent probes, drug delivery and biological imaging. Nevertheless, the metabolism of ND and its derivatives is not yet well understood for diverse biomedical applications. In this study, to evaluate the cytotoxicity and biodistribution of the ND derivative, pure ND was functionalized with ethylenediamine (EDA) as a linker (ND-EDA) and then covalently labeled with an organic fluorescent dye called calcein. The modified ND is referred to as ND-EDA-calcein. Even at the maximum dose of 0.1 mg & BULL;mL(-1), the in vitro results for ND-EDA-calcein against HepG2 and LO2 cell lines showed negligible cytotoxicity, which was in line with the in vivo observations. In addition, it was found that following an intraperitoneal injection, ND-EDA-calcein could be accumulated mostly in the liver and kidney of the mice but not in the spleen. It is interesting to note that after 5 days following intraperitoneal injection, mice can totally metabolize the elimination of ND-EDA-calcein outside of their bodies. Therefore, our findings suggested that NDs can be considered for various nanomedicine applications including drug delivery and biomarkers.
作者机构:
[Fu, Lijie; Liu, Fanglei; Zhao, Jiayi; Yang, Guohai; Qu, Lulu; Gu, Yingqiu] Jiangsu Normal Univ, Sch Chem & Mat Sci, Xuzhou 221116, Peoples R China.;[Zhu, Chengzhou] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Natl Key Lab Green Pesticide, Wuhan 430079, Peoples R China.;[Zhu, Jun-Jie] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210093, Peoples R China.;[Lin, Yuehe] Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA.
通讯机构:
[Lulu Qu] S;[Chengzhou Zhu] N;School of Chemistry and Materials Science, Jiangsu Normal University, Xuzhou 221116, PR China<&wdkj&>National Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P.R. China
摘要:
As recently developed two-dimensional (2D) materials, MXenes have attracted great attention in the fields of sensing, catalysis, energy, and electromagnetism due to their good electrical, mechanical, and optical properties and their biocompatibility. Their rich elemental composition, diverse surface functional groups and flexible bandgaps enable them to exhibit ideal physicochemical properties that are superior to those of traditional 2D materials. Therefore, MXenes not only serve as ideal biosensing materials but also show great potential in biomedical-related applications. In this review, we summarize the latest research on MXenes in the fields of biosensing and biomedicine. First, we introduce the different synthe-sis strategies and unique properties of MXenes. Then, according to the sensing mechanism, we divide the biosensing applications of MXenes into three subcategories: electrochemical, photochemical, and opto-electrochemical biosensors. Moreover, we systematically evaluate the biomedical applications of MXenes from four aspects: antibacterial activity, bioimaging, therapeutics and theranostics. How the specific chemical and physical properties of MXenes that can influence the efficiency of biosensing and biomedicine are highlighted, as well as the structure-performance relationships determining detection sensitivity and the toxicity of materials. Finally, we discuss the recent challenges and future prospects and opportunities for MXenes. It is expected that the review could promote the development of related fields.(c) 2022 Elsevier B.V. All rights reserved.
摘要:
Removing microcystins (MCs) safely and effectively has become an urgent global problem because of their extremely hazardous to the environment and public health. Microcystinases derived from indigenous microorganisms have received widespread attention due to their specific MC biodegradation function. However, linearized MCs are also very toxic and need to be removed from the water environment. How MlrC binds to linearized MCs and how it catalyzes the degradation process based on the actual three-dimensional structure have not been determined. In this study, the binding mode of MlrC with linearized MCs was explored using a combination of molecular docking and site-directed mutagenesis methods. A series of key substrate binding residues, including E70, W59, F67, F96, S392 and so on, were identified. Sodium dodecane sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) was used to analyze samples of these variants. The activity of MlrC variants were measured using high performance liquid chromatography (HPLC). We used fluorescence spectroscopy experiments to research the relationship between MlrC enzyme (E), zinc ion (M), and substrate (S). The results showed that MlrC enzyme, zinc ion and substrate formed E-M-S intermediates during the catalytic process. The substrate-binding cavity was made up of N and C-terminal domains and the substrate-binding site mainly included N41, E70, D341, S392, Q468, S485, R492, W59, F67, and F96. The E70 residue involved in both substrate catalysis and substrate binding. In conclusion, a possible catalytic mechanism of the MlrC enzyme was further proposed based on the experimental results and a literature survey. These findings provided new insights into the molecular mechanisms of the MlrC enzyme to degrade linearized MCs, and laid a theoretical foundation for further biodegradation studies of MCs.