Discovery of New 2-[(4,6-Dimethoxy-1,3,5-triazin-2-yl)oxy]-6-(substituted phenoxy)benzoic Acids as Flexible Inhibitors of Arabidopsis thaliana Acetohydroxyacid Synthase and Its P197L Mutant
作者：
Qu, Ren-Yu;Yang, Jing-Fang;Devendar, Ponnam;Kang, Wei-Ming;Liu, Yu-Chao;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(51):11170-11178 ISSN：0021-8561
通讯作者：
Yang, Guang-Fu;Xi, Zhen
作者机构：
[Yang, Guang-Fu; Devendar, Ponnam; Kang, Wei-Ming; Liu, Yu-Chao; Qu, Ren-Yu; Chen, Qiong; Yang, Jing-Fang] Cent China Normal Univ CCNU, Minist Educ, Key Lab Pesticide & Chem Biol, Coll Chem, Wuhan 430079, Hubei, Peoples R China.;[Xi, Zhen; Niu, Cong-Wei] Nankai Univ NKU, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.;[Yang, Guang-Fu; Xi, Z; Yang, GF; Xi, Zhen] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
通讯机构：
[Yang, Guang-Fu; Xi, Z; Yang, GF] C;[Xi, Zhen] N;Cent China Normal Univ CCNU, Minist Educ, Key Lab Pesticide & Chem Biol, Coll Chem, Wuhan 430079, Hubei, Peoples R China.;Nankai Univ NKU, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.;Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
关键词：
AHAS inhibitors;P197L mutant;antiresistance property;herbicidal activity;molecular docking;structure-activity relationship
摘要：
In the search for new antiresistance acetohydroxyacid synthase (AHAS, EC 2.2.1.6) inhibitors to combat weed resistance associated with AHAS mutations, a series of 2-[(4,6-dimethoxy-1,3,5-triazin-2-yl)oxy]-6-(substituted phenoxy)benzoic acids 11-38 were designed and synthesized via the strategy of conformational flexibility analysis. Compounds 21, 22, 26, 33, 36, and 38 with high potency against both wild-type AtAHAS and its P197L mutant were identified as promising candidates with low resistance factors (RF, defined as the ratio between the k<inf>i</inf>values toward P197L mutant and wild-type AHAS) ranging from 0.73 to 6.32. Especially, compound 22 (RF = 0.73) was further identified as the most potent antiresistance AHAS inhibitor because of its significantly reduced resistance level compared with that of tribenuron-methyl (RF = 2650) and bispyribac (RF = 4.57). Furthermore, compounds 26, 33, 36, and 38 also displayed promising herbicidal activities against sensitive and resistant (P197L) Descurainia sophia at the dosage of 75-150 g of active ingredient (ai)/ha. Notably, compounds 33 and 38 still maintained over 60% herbicidal activity toward the resistant weed even at much lower dosages (37.5 g ai/ha). Therefore, the designed scaffold has the great potential to discover new candidate compounds for the control of weed resistance associated with AHAS mutation.<br/> ©2017 American Chemical Society.
语种：
英文
展开
含咪唑啉酮结构的吡唑类对羟基苯基丙酮酸双加氧酶抑制剂的除草活性及选择性研究
作者：
徐玉玲;林红艳;吴丰旭;康德来;杨文超;...
期刊：
农药学学报 ,2017年19(4):418-427 ISSN：1008-7303
作者机构：
武汉轻工大学化学与环境工程学院, 农药与化学生物学教育部重点实验室, 武汉, 430023;华中师范大学化学学院, 农药与化学生物学教育部重点实验室, 武汉, 430079;武汉轻工大学化学与环境工程学院, 武汉, 430023;[杨光富; 林红艳; 吴丰旭; 杨文超] 华中师范大学化学学院, 农药与化学生物学教育部重点实验室, 武汉, 430079;[徐玉玲] 武汉轻工大学化学与环境工程学院, 农药与化学生物学教育部重点实验室, 武汉, 430023
关键词：
咪唑啉酮;对羟基苯基丙酮酸双加氧酶;除草剂;除草活性;作物安全性
摘要：
对羟基苯基丙酮酸双加氧酶(HPPD)是一种重要的除草剂作用靶标。为了发现具有高活性和高选择性的新型HPPD抑制型除草剂,对前期合成的23个含咪唑啉酮结构单元的吡唑类衍生物(2A~2W)进行了深入的生物活性评价和构效关系研究,比较了它们对拟南芥HPPD(AtHPPD)和人源HPPD(hHPPD)抑制活性的差异,从酶水平上总结了该类化合物的结构-活性关系和种属选择性规律,从活体植株水平研究了它们的除草活性。结果表明:部分化合物表现出良好的除草活性和作物安全性,其中化合物2E和2G在150 g/hm~2剂量下对荠菜、繁缕、小藜和棒头草抑制活性达到80%以上,且其对作物的安全性也明显优于商品化除草剂硝磺草酮。此外,化合物2P在酶水平上的选择性倍数高达93倍,展示出良好的应用潜力。
语种：
中文
展开
Synthesis and Herbicidal Activity of Pyrido[2,3-d]pyrimidine-2,4-dione-Benzoxazinone Hybrids as Protoporphyrinogen Oxidase Inhibitors
作者：
Wang, Da-Wei;Li, Qian;Wen, Kai;Ismail, Ismail;Liu, Dan-Dan;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(26):5278-5286 ISSN：0021-8561
通讯作者：
Xi, Zhen;Yang, Guang-Fu
作者机构：
[Li, Qian; Wen, Kai; Xi, Zhen; Niu, Cong-Wei; Wen, Xin; Liu, Dan-Dan; Wang, Da-Wei; Ismail, Ismail] Nankai Univ, State Key Lab Elementoorgan Chem, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China.;[Li, Qian; Wen, Kai; Xi, Zhen; Niu, Cong-Wei; Wen, Xin; Liu, Dan-Dan; Wang, Da-Wei; Ismail, Ismail] Nankai Univ, Dept Chem Biol, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China.;[Yang, Guang-Fu] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Xi, Zhen] N;[Yang, Guang-Fu] C;Nankai Univ, State Key Lab Elementoorgan Chem, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China.;Nankai Univ, Dept Chem Biol, Coll Chem, Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 300071, Peoples R China.;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
benzoxazinone;herbicide;protoporphyrinogen oxidase;pyrido[2,3-d]pyrimidine-2,4-dione;structure-activity relationship
摘要：
To search for new protoporphyrinogen oxidase (PPO, EC 1.3.3.4) inhibitors with improved bioactivity, a series of novel pyrido[2,3-d]pyrimidine-2,4-dione-benzoxazinone hybrids, 9-13, were designed and synthesized. Several compounds with improved tobacco PPO (mtPPO)-inhibiting and promising herbicidal activities were found. Among them, the most potent compound, 3-(7-fluoro-3-oxo-4-(prop-2-yn-1-yl)-3,4-dihydro-2H-benzo[b][1,4] oxazin-6-yl)-1-methylpyrido[2,3-d]pyrimidine-2,4(1H,3H)-dione, 11q, with a K<inf>i</inf>value of 0.0074 μM, showed six times more activity than flumioxazin (K<inf>i</inf>= 0.046 μM) against mtPPO. Compound 11q displayed a strong and broad spectrum of weed control at 37.5-150 g of active ingredient (ai)/ha by both post- and pre-emergence application, which was comparable to that of flumioxazin. 11q was safe to maize, soybean, peanut, and cotton at 150 g ai/ha, and selective to rice and wheat at 75 g ai/ha by pre-emergence application, indicating potential applicability in these fields. ©2017 American Chemical Society.
语种：
英文
展开
4-Hydroxyphenylpyruvate dioxygenase inhibitors: From chemical biology to agrochemicals
作者：
Ndikuryayo, Ferdinand;Moosavi, Behrooz;Yang, Wen-Chao
* ;Yang, Guang-Fu
* （ 期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2017年65(39):8523-8537 ISSN：0021-8561
通讯作者：
Yang, Wen-Chao;Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Ndikuryayo, Ferdinand; Moosavi, Behrooz; Yang, Wen-Chao; Yang, WC; Yang, GF] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;[Yang, Guang-Fu] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
通讯机构：
[Yang, WC; Yang, GF; Yang, Guang-Fu] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Hubei, Peoples R China.;Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
关键词：
4-hydroxyphenylpyruvate dioxygenase;agrochemical;chemical biology;herbicide;inhibitor
摘要：
The development of new herbicides is receiving considerable attention to control weed biotypes resistant to current herbicides. Consequently, new enzymes are always desired as targets for herbicide discovery. 4-Hydroxyphenylpyruvate dioxygenase (HPPD, EC 1.13.11.27) is an enzyme engaged in photosynthetic activity and catalyzes the transformation of 4-hydroxyphenylpyruvic acid (HPPA) into homogentisic acid (HGA). HPPD inhibitors constitute a promising area of discovery and development of innovative herbicides with some advantages, including excellent crop selectivity, low application rates, and broad-spectrum weed control. HPPD inhibitors have been investigated for agrochemical interests, and some of them have already been commercialized as herbicides. In this review, we mainly focus on the chemical biology of HPPD, discovery of new potential inhibitors, and strategies for engineering transgenic crops resistant to current HPPD-inhibiting herbicides. The conclusion raises some relevant gaps for future research directions.
语种：
英文
展开
Discovery of Potent Succinate-Ubiquinone Oxidoreductase Inhibitors via Pharmacophore-linked Fragment Virtual Screening Approach
作者：
Xiong, Li;Zhu, Xiao-Lei
* ;Gao, Hua-Wei;Fu, Yu
（ ;Hu, Sheng-Quan;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2016年64(24):4830-4837 ISSN：0021-8561
通讯作者：
Zhu, Xiao-Lei;Yang, Guang-Fu
作者机构：
[Fu, Yu; Hu, Sheng-Quan; Zhu, Xiao-Lei; Xiong, Li; Yang, Wen-Chao; Gao, Hua-Wei; Jiang, Li-Na] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Fu, Yu] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.;[Zhu, XL; Yang, GF] Ctr China Normal Univ, Luoyu Rd 152, Wuhan 43007, Peoples R China.
通讯机构：
[Zhu, XL; Yang, GF] C;Ctr China Normal Univ, Luoyu Rd 152, Wuhan 43007, Peoples R China.
关键词：
succinate-ubiquinone oxidoreductase;complex II;pharmacophore-linked fragment virtual screening;molecular modeling;structure-based design
摘要：
Succinate-ubiquinone oxidoreductase (SQR) is an attractive target for fungicide discovery. Herein, we report the discovery of novel SQR inhibitors using a pharmacophore-linked fragment virtual screening approach, a new drug design method developed in our laboratory. Among newly designed compounds, compound 9s was identified as the most potent inhibitor with a K<inf>i</inf>value of 34 nM against porcine SQR, displaying approximately 10-fold higher potency than that of the commercial control penthiopyrad. Further inhibitory kinetics studies revealed that compound 9s is a noncompetitive inhibitor with respect to the substrate cytochrome c and DCIP. Interestingly, compounds 8a, 9h, 9j, and 9k exhibited good in vivo preventive effects against Rhizoctonia solani. The results obtained from molecular modeling showed that the orientation of the R<sup>2</sup>group had a significant effect on binding with the protein. ©2016 American Chemical Society.
语种：
英文
展开
Recent advances in cytokine detection by immunosensing
作者：
Liu, Guozhen
* ;Qi, Meng;Hutchinson, Mark R.;Yang, Guangfu
（ ;Goldys, Ewa M.
* 期刊：
Biosensors and Bioelectronics ,2016年79:810-821 ISSN：0956-5663
通讯作者：
Liu, Guozhen;Goldys, Ewa M.
作者机构：
[Yang, Guangfu; Liu, Guozhen; Qi, Meng] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Goldys, Ewa M.; Liu, Guozhen] Macquarie Univ, ARC Ctr Excellence Nanoscale Biophoton CNBP, N Ryde, NSW 2109, Australia.;[Hutchinson, Mark R.] Univ Adelaide, ARC Ctr Excellence Nanoscale Biophoton CNBP, Adelaide, SA 5005, Australia.
通讯机构：
[Liu, Guozhen] C;[Goldys, Ewa M.] M;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Macquarie Univ, ARC Ctr Excellence Nanoscale Biophoton CNBP, N Ryde, NSW 2109, Australia.
关键词：
Cytokines;Immunosensing;Microfluidics;Real-time detection;Review;Sensitivity
摘要：
The detection of cytokines in body fluids, cells, tissues and organisms continues to attract considerable attention due to the importance of these key cell signaling molecules in biology and medicine. In this review, we describe recent advances in cytokine detection in the course of ongoing pursuit of new analytical approaches for these trace analytes with specific focus on immunosensing. We discuss recent elegant designs of sensing interface with improved performance with respect to sensitivity, selectivity, stability, simplicity, and the absence of sample matrix effects. Various immunosensing approaches based on multifunctional nanomaterials open novel opportunities for ultrasensitive detection of cytokines in body fluids in vitro and in vivo. Methodologies such as suspension arrays also known as bead assays together with optical fiber-based sensors, on their own or in combination with microfluidic devices will continue to have an important role to address the grand challenge of real-time in vivo multiplex cytokine detection. © 2016 Elsevier B.V.
语种：
英文
展开
吡唑类HPPD除草剂的最新研究进展
作者机构：
[杨光富; 何波; 王大伟; 陈琼] 华中师范大学化学学院农药与化学生物学教育部重点实验室;[杨光富; 何波; 王大伟; 陈琼] 南开大学化学学院元素有机化学国家重点实验室
会议名称：
中国化工学会农药专业委员会第十七届年会
会议时间：
2016-07-28
会议地点：
中国河南郑州
关键词：
对羟基苯丙酮酸双加氧化酶;作用机制;结合位点;除草剂
摘要：
对羟基苯丙酮酸双加氧化酶(EC 1.13.11.27,HPPD)作为除草剂靶标,具有高效,低毒,作物安全性高,不易产生抗性、环境相容性好及对后茬作物安全等一系列优点。本文详细介绍了HPPD的结构、抑制剂的作用位点以及其在生物体内的作用机制和代谢产物,同时对商品化吡唑类HPPD除草剂的特点及目前的研究现状进行总结分析。
语种：
中文
展开
Detection of Intracellular Selenol-Containing Molecules Using a Fluorescent Probe with Near-Zero Background Signal
作者：
Sun, Qi;Yang, Shu-Hou;Wu, Lei;Dong, Qing-Jian;Yang, Wen-Chao* ;...
期刊：
Analytical Chemistry ,2016年88(11):6084-6091 ISSN：0003-2700
通讯作者：
Yang, Wen-Chao;Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Yang, Wen-Chao; Sun, Qi; Yang, Shu-Hou; Wu, Lei; Yang, WC; Yang, GF] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Dong, Qing-Jian] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Nucl Med, Tongji Med Coll, Wuhan 430030, Peoples R China.
通讯机构：
[Yang, WC; Yang, GF] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
摘要：
Selenocysteine (Sec), encoded as the 21st amino acid, is the predominant chemical form of selenium that is closely related to various human diseases. Thus, it is of high importance to identify novel probes for sensitive and selective recognition of Sec and Sec-containing proteins. Although a few probes have been reported to detect artificially introduced selenols in cells or tissues, none of them has been shown to be sensitive enough to detect endogenous selenols. We report the characterization and application of a new fluorogenic molecular probe for the detection of intracellular selenols. This probe exhibits near-zero background fluorescence but produces remarkable fluorescence enhancement upon reacting with selenols in a fast chemical reaction. It is highly specific and sensitive for intracellular selenium-containing molecules such as Sec and selenoproteins. When combined with flow cytometry, this probe is able to detect endogenous selenols in various human cancer cells. It is also able to image endogenous selenol-containing molecules in zebrafish under a fluorescence microscope. These results demonstrate that this molecular probe can function as a useful molecular tool for intracellular selenol sensing, which is valuable in the clinical diagnosis for human diseases associated with Sec-deficiency or overdose. © 2016 American Chemical Society.
语种：
英文
展开
An Update on Poly(ADP-ribose)polymerase-1 (PARP-1) Inhibitors: Opportunities and Challenges in Cancer Therapy
作者：
Wang, Ying-Qing;Wang, Ping-Yuan;Wang, Yu-Ting;Yang, Guang-Fu
（ ;Zhang, Ao
* ;...
期刊：
JOURNAL OF MEDICINAL CHEMISTRY ,2016年59(21):9575-9598 ISSN：0022-2623
通讯作者：
Miao, Ze-Hong;Zhang, Ao
作者机构：
[Miao, Ze-Hong; Wang, Ying-Qing; Wang, Yu-Ting] Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China.;[Zhang, Ao; Wang, Ping-Yuan] Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Lu,Bldg 3,Room 426, Shanghai 201203, Peoples R China.;[Zhang, Ao; Wang, Ping-Yuan] Chinese Acad Sci, Shanghai Inst Mat Med, Synthet Organ & Med Chem Lab, 555 Zuchongzhi Lu,Bldg 3,Room 426, Shanghai 201203, Peoples R China.;[Yang, Guang-Fu; Wang, Ping-Yuan] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
通讯机构：
[Miao, Ze-Hong; Zhang, Ao] C;Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China.;Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Lu,Bldg 3,Room 426, Shanghai 201203, Peoples R China.;Chinese Acad Sci, Shanghai Inst Mat Med, Synthet Organ & Med Chem Lab, 555 Zuchongzhi Lu,Bldg 3,Room 426, Shanghai 201203, Peoples R China.
摘要：
Poly(ADP-ribose)polymerase-1 (PARP-1) is a critical DNA repair enzyme in the base excision repair pathway. Inhibitors of this enzyme comprise a new type of anticancer drug that selectively kills cancer cells by targeting homologous recombination repair defects. Since 2010, important advances have been achieved in PARP-1 inhibitors. Specifically, the approval of olaparib in 2014 for the treatment of ovarian cancer with BRCA mutations validated PARP-1 as an anticancer target and established its clinical importance in cancer therapy. Here, we provide an update on PARP-1 inhibitors, focusing on breakthroughs in their clinical applications and investigations into relevant mechanisms of action, biomarkers, and drug resistance. We also provide an update on the design strategies and the structural types of PARP-1 inhibitors. Opportunities and challenges in PARP-1 inhibitors for cancer therapy will be discussed based on the above advances.
语种：
英文
展开
An ionic liquid promoted approach to bitriazolyl compounds as succinate-ubiquinone oxidoreductase inhibitors
作者：
Zhang, Rui;Wu, Qiong-You
* ;Tao, Jun;Pan, Jin-Huan;Yang, Guang-Fu
* （ 期刊：
NEW JOURNAL OF CHEMISTRY ,2016年41(1):204-211 ISSN：1144-0546
通讯作者：
Wu, Qiong-You;Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Wu, Qiong-You; Pan, Jin-Huan; Tao, Jun; Yang, GF; Zhang, Rui] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wu, QY; Yang, GF] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
The respiratory chain succinate-ubiquinone oxidoreductase (SQR or complex II) is a promising target for fungicide discovery. As a continuation of our research work on the development of new fungicides, a series of bitriazolyl compounds were designed and synthesized in excellent yields by an ionic liquid promoted 1,3-dipolar Huisgen cycloaddition reaction of azides and akynes. These newly synthesized compounds were characterized by H-1 NMR, C-13 NMR and HR-MS spectroscopy. The in vitro assay indicated that several compounds displayed good inhibitory effects against porcine succinate-cyctochrome reductase (SCR) with IC50 values ranging from 2.89 to 61.19 mu M. Compound 1b with an IC50 value of 2.89 mu M, comparable to the commercial control penthiopyrad, was identified as the most promising inhibitor. Further evaluation of the representative compounds against respective SQR and cyt bc(1) indicated that their inhibitory potency against SQR was much higher than that against cyt bc(1), suggesting that SQR might be a potential target of these inhibitors. Furthermore, molecular docking studies suggested that strong hydrogen bonding and pi-pi stacking interactions might be responsible for a higher SQR inhibitory effect of compound 1b as compared to that of compounds 1d and 2b. Consequently, bitriazolyl compounds, a totally new skeleton that is distinct from the existing commercial SQR-inhibiting fungicides, were discovered, which could potentially be a new lead for further development of SQR inhibitors.
语种：
英文
展开
Synthesis, Herbicidal Activity, and QSAR of Novel N-Benzothiazolyl-pyrimidine-2,4-diones as Protoporphyrinogen Oxidase Inhibitors
作者：
Zuo, Yang;Wu, Qiongyou;Su, Sun-Wen;Niu, Cong-Wei;Xi, Zhen;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2016年64(3):552-562 ISSN：0021-8561
通讯作者：
Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Su, Sun-Wen; Zuo, Yang; Wu, Qiongyou] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Niu, Cong-Wei; Xi, Zhen] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.
通讯机构：
[Yang, Guang-Fu] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
benzothiazole;herbicide;molecular design;protoporphyrinogen oxidase;pyrimidinedione
摘要：
Protoporphyrinogen oxidase (PPO, E.C. 1.3.3.4) is known as a key action target for several structurally diverse herbicides. As a continuation of our research work on the development of new PPO-inhibiting herbicides, a series of novel 3-(2′-halo-5′-substituted-benzothiazol-1′-yl)-1-methyl-6-(trifluoromethyl)pyrimidine-2,4-diones 9 were designed and synthesized. The bioassay results indicated that a number of the newly synthesized compounds exhibited higher inhibition activity against tobacco PPO (mtPPO) than the controls, saflufenacil and sulfentrazone. Compound 9F-5 was identified as the most potent inhibitor with a K<inf>i</inf>value of 0.0072 ΜM against mtPPO, showing about 4.2-fold and 1.4-fold higher potency than sulfentrazone (K<inf>i</inf>= 0.03 ΜM) and saflufenacil (K<inf>i</inf>= 0.01 ΜM), respectively. An additional green house assay demonstrated that compound 9F-6 (K<inf>i</inf>= 0.012 ΜM) displayed the most promising postemergence herbicidal activity with a broad spectrum even at a concentration as low as 37.5 g of active ingredient (ai)/ha. Maize exhibits relative tolerance against compound 9F-6 at the dosage of 150 g ai/ha, but it is susceptible to saflufenacil even at 75 g ai/ha. Thus, compound 9F-6 exhibits the potential to be a new herbicide for weed control in maize fields. ©2016 American Chemical Society.
语种：
英文
展开
Discovery of cytochrome bc1 complex inhibitors inspired by the natural product karrikinolide
作者：
Chen, Cheng;Wu, Qiong-You* ;Shan, Lian-Ying;Zhang, Bei;Verpoort, Francis;...
期刊：
RSC Advances ,2016年6(100):97580-97586 ISSN：2046-2069
通讯作者：
Wu, Qiong-You;Yang, Guang-Fu
作者机构：
[Chen, Cheng; Verpoort, Francis] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Wuhan 430070, Peoples R China.;[Yang, Guang-Fu; Shan, Lian-Ying; Wu, Qiong-You; Yang, GF; Zhang, Bei] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wu, QY; Yang, GF] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
摘要：
The cytochrome bc<inf>1</inf>complex (cyt bc<inf>1</inf>or complex III) is a promising target of numerous antibiotics and fungicides. With an aim to indentify new lead structures for the bc<inf>1</inf>complex, a series of novel inhibitors were discovered from the natural product karrikinolide for the first time. Extensive screening results suggested variable inhibitory activities of these compounds against succinate-cytochrome reductase [SCR, a mixture of respiratory complex II (SQR) and complex III (the bc<inf>1</inf>complex)], implying the essential role of a 4-substituted phenyl group for the high potency. Exceptionally, compound 12g showed excellent inhibition potency having an IC<inf>50</inf>value in the sub-micromolar range, demonstrating its higher potency than the commercial control amisulbrom by over two orders of magnitude. Further experiments inferred that these newly prepared compounds mainly target the bc<inf>1</inf>complex. Seemingly, this work has presented a new lead scaffold for further development of bc<inf>1</inf>complex inhibitors.<br/> ©2016 The Royal Society of Chemistry.
语种：
英文
展开
含苯并2,2-二氧噻二唑结构的吡唑类HPPD抑制剂的设计、合成及其生物活性研究
作者：
杨文超;徐玉玲;林红艳;武园;杨光富
（ 期刊：
中国科学：化学 ,2016年46(11):1180-1187 ISSN：1674-7224
作者机构：
华中师范大学化学学院, 农药与化学生物学教育部重点实验室, 武汉, 430079;华中师范大学化学学院, 农药与化学生物学教育部重点实验室;[杨光富] 化学化工协同创新中心(天津), 武汉, 430079;[杨光富] 华中师范大学化学学院, 农药与化学生物学教育部重点实验室;[杨文超; 徐玉玲; 林红艳; 武园] 华中师范大学化学学院, 农药与化学生物学教育部重点实验室, 武汉, 430079
关键词：
除草剂;对羟基苯基丙酮酸双加氧酶;抑制活性;硝磺草酮
摘要：
对羟基苯基丙酮酸双加氧酶是一种重要的除草剂靶标.本文基于活性亚结构拼接的策略设计合成了一系列含有苯并2,2-二氧噻二唑的吡唑类新型对羟基苯基丙酮酸双加氧酶(HPPD)抑制剂.随后分别从酶水平和活体植株水平研究了它们的生物活性,总结了其结构与活性关系.部分化合物表现出很高的HPPD酶抑制活性和活体除草活性,化合物6d和6j对冬季阔叶杂草表现出良好的防治效果,且其作物安全性也明显优于对照药剂硝磺草酮,展示出良好的应用潜力.
语种：
中文
展开
Actin, Membrane Trafficking and the Control of Prion Induction, Propagation and Transmission in Yeast
作者：
Moosavi, Behrooz
* ;Mousavi, Bibimaryam;Yang, Guang-Fu
（ 期刊：
Traffic ,2016年17(1):5-20 ISSN：1398-9219
通讯作者：
Moosavi, Behrooz
作者机构：
[Yang, Guang-Fu; Moosavi, Behrooz] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Mousavi, Bibimaryam] Wuhan Univ Technol, State Key Lab Adv Technol Mat Synth & Proc, Lab Organometall Catalysis & Ordered Mat, Wuhan 430070, Peoples R China.
通讯机构：
[Moosavi, Behrooz] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.
关键词：
[PSI+];actin;endocytosis;IPOD;prion;yeast
摘要：
The model eukaryotic yeast Saccharomyces cerevisiae has proven a useful model system in which prion biogenesis and elimination are studied. Several yeast prions exist in budding yeast and a number of studies now suggest that these alternate protein conformations may play important roles in the cell. During the last few years cellular factors affecting prion induction, propagation and elimination have been identified. Amongst these, proteins involved in the regulation of the actin cytoskeleton and dynamic membrane processes such as endocytosis have been found to play a critical role not only in facilitating de novo prion formation but also in prion propagation. Here we briefly review prion formation and maintenance with special attention given to the cellular processes that require the functionality of the actin cytoskeleton. © 2016 John Wiley & Sons A/S.
语种：
英文
展开
线粒体呼吸链复合物的结构与作用机理研究
作者机构：
[杨光富; 张蓓; 杨文超] 华中师范大学化学学院农药与化学生物学教育部重点实验室
会议名称：
中国化学会第30届学术年会
会议时间：
2016-07-01
会议地点：
大连
会议论文集名称：
中国化学会第30届学术年会论文集
关键词：
杀菌剂;线粒体呼吸链复合物;抑制剂;作用机理;合理设计
摘要：
人口增长是新世界人类面临的三大挑战之一,也是我国的基本国情。而人口的持续增长也在不断提高对粮食产量的需求,而农药在确保粮食安全方面发挥着不可替代的作用,是关系国家经济社会发展的重要战略物质。但近年来植物病原菌对我国农业生产的危害极为严重,呈现出逐年增加的趋势,严重制约着粮食产量的提高。化学杀菌剂是目前防控植物病害最经济有效的手段,线粒体呼吸链复合物则是当前最具发展前景的农用杀菌剂靶标之一,因此对线
语种：
中文
展开
基于细胞色素bc1复合物的虚拟筛选
作者机构：
[杨光富; 陶俊; 朱晓磊] 华中师范大学化学学院农药与化学生物学教育部重点实验室
会议名称：
中国化学会第30届学术年会
会议时间：
2016-07-01
会议地点：
大连
会议论文集名称：
中国化学会第30届学术年会论文集
关键词：
Strobilurin类杀菌剂;抗性;灰霉菌;同源模建;虚拟筛选
摘要：
Strobilurin类杀菌剂是继苯并咪唑和***类之后杀菌剂研发史上的又一里程碑,靶向线粒体复合物Ⅲ的Qo位点。然而,病原菌对该类杀菌剂抗性问题使得该类杀菌剂的适用收到很大的限制,更为严重的是G143A突变几乎对所有Strobilurin类杀菌剂均产生抗性,因此设计合成新型复合物Ⅲ抑制剂时必须要考虑如何规避G143A突变而导致的抗药性。Pyribencarb是一种新型苄基氨基甲酸酯类杀菌剂,该杀菌
语种：
中文
展开
Target-oriented Structural Optimization of Natural Products
作者：
Cheng Chen;Xiao-Lei Zhu;Guang-Fu Yang
（ 作者机构：
[Guang-Fu Yang; Cheng Chen; Xiao-Lei Zhu] Key Laboratory of Pesticide & Chemical Biology,Ministry of Education,College of Chemistry,Central China Normal University
会议名称：
中国化学会第30届学术年会-第二十八分会：化学生物学
会议时间：
2016-07-01
会议地点：
中国辽宁大连
摘要：
Natural products invariably show a broad range of interesting and useful biological activities.Most of successful drug and pesticide are analogs of natural products.These analogs are valuable since th
语种：
英文
展开
An Efficient One-Pot Synthesis of 2-(Aryloxyacetyl)cyclohexane-1,3-diones as Herbicidal 4-Hydroxyphenylpyruvate Dioxygenase Inhibitors
作者：
Wang, Da-Wei;Lin, Hong-Yan;He, Bo;Wu, Feng-Xu;Chen, Tao;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2016年64(47):8986-8993 ISSN：0021-8561
通讯作者：
Yang, Wen-Chao;Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Yang, Wen-Chao; Chen, Tao; Chen, Qiong; Wang, Da-Wei; Lin, Hong-Yan; Wu, Feng-Xu; He, Bo; Yang, WC; Yang, GF] Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;[Yang, Guang-Fu] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
通讯机构：
[Yang, WC; Yang, GF; Yang, Guang-Fu] C;Cent China Normal Univ, Coll Chem, Key Lab Pesticide & Chem Biol, Minist Educ, Wuhan 430079, Peoples R China.;Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
关键词：
*4-hydroxyphenylpyruvate dioxygenase;*herbicidal activity;*one-pot synthesis;*structure-activity relationship;*triketone
摘要：
4-Hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, HPPD) is an important target for new bleaching herbicides discovery. As a continuous work to discover novel crop selective HPPD inhibitor, a series of 2-(aryloxyacetyl)cyclohexane-1,3-diones were rationally designed and synthesized by an efficient one-pot procedure using N,N′-carbonyldiimidazole (CDI), triethylamine, and acetone cyanohydrin in CH<inf>2</inf>Cl<inf>2</inf>. A total of 58 triketone compounds were synthesized in good to excellent yields. Some of the triketones displayed potent in vitro Arabidopsis thaliana HPPD (AtHPPD) inhibitory activity. 2-(2-((1-Bromonaphthalen-2-yl)oxy)acetyl)-3-hydroxycyclohex-2-en-1-one, II-13, displayed high, broad-spectrum, and postemergent herbicidal activity at the dosage of 37.5-150 g ai/ha, nearly as potent as mesotrione against some weeds. Furthermore, II-13 showed good crop safety against maize and canola at the rate of 150 g ai/ha, indicating that II-13 might have potential as a herbicide for weed control in maize and canola fields. II-13 is the first HPPD inhibitor showing good crop safety toward canola. ©2016 American Chemical Society.
语种：
英文
展开
Triazolopyrimidines as a New Herbicidal Lead for Combating Weed Resistance Associated with Acetohydroxyacid Synthase Mutation
作者：
Liu, Yu-Chao;Qu, Ren-Yu;Chen, Qiong;Yang, Jing-Fang;Cong-Wei, Niu;...
期刊：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ,2016年64(24):4845-4857 ISSN：0021-8561
通讯作者：
Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Yang, Jing-Fang; Liu, Yu-Chao; Chen, Qiong] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Yang, Guang-Fu; Zhen, Xi; Cong-Wei, Niu] Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.;[Yang, Guang-Fu; Zhen, Xi] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
通讯机构：
[Yang, Guang-Fu] C;[Yang, Guang-Fu] N;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Nankai Univ, State Key Lab Elementoorgan Chem, Tianjin 300071, Peoples R China.;Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
关键词：
molecular design;herbicide;weed resistance;acetohydroxyacid synthase;triazolopyrimidines
摘要：
Acetohydroxyacid synthase (AHAS;also known as acetolactate synthase;EC 2.2.1.6, formerly EC 4.1.3.18) is the first common enzyme in the biosynthetic pathway leading to the branched-chain amino acids in plants and a wide range of microorganisms. Weed resistance to AHAS-inhibiting herbicides, increasing at an exponential rate, is becoming a global problem and leading to an urgent demand of developing novel compounds against both resistant and wild AHAS. In the present work, a series of novel 2-aroxyl-1,2,4-triazolopyrimidine derivatives (a total of 55) were designed and synthesized with the aim to discover an antiresistant lead compound. Fortunately, the screening results indicated that many of the newly synthesized compounds showed a better, even excellent, inhibition effect against both the wild-type Arabidopsis thaliana AHAS and P197L mutants. Among them, compounds 5-3 to 5-17, compounds 5-19 to 5-26, compounds 5-28 to 5-45, and compound 5-48 have the lower values of resistance factor (RF) and display a potential power to overcome resistance associated with the P197L mutation in the enzyme levels. Further greenhouse in vivo assay showed that compounds 5-15 and 5-20 displayed "moderate" to "good" herbicidal activity against both the wild type-and the resistant (P197L mutation) Descurainia sophia, even at a rate as low as 0.9375 (g of ai/ha). The above results indicated that these two compounds could be used as new leads for the future development of antiresistance herbicides. ©2016 American Chemical Society.
语种：
英文
展开
A Highly Sensitive and Selective Fluorescent Probe for Thiophenol Designed via a Twist-Blockage Strategy
作者：
Sun, Qi;Yang, Shu-Hou;Wu, Lei;Yang, Wen-Chao
* ;Yang, Guang-Fu
* （ 期刊：
Analytical Chemistry ,2016年88(4):2266-2272 ISSN：0003-2700
通讯作者：
Yang, Wen-Chao;Yang, Guang-Fu
作者机构：
[Yang, Guang-Fu; Yang, Wen-Chao; Sun, Qi; Yang, Shu-Hou; Wu, Lei; Yang, WC; Yang, GF] Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;[Yang, Guang-Fu] Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
通讯机构：
[Yang, WC; Yang, GF; Yang, Guang-Fu] C;Cent China Normal Univ, Coll Chem, Minist Educ, Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.;Collaborat Innovat Ctr Chem Sci & Engn, Tianjin 30071, Peoples R China.
摘要：
A benzoquinolizine coumarin-based fluorescent probe was developed for detecting thiophenols, demonstrating the superior fluorescence properties caused by the decay of the twisting effect of N,N-diethylamino group of coumarin. It discriminated thiophenols from various analytes including aliphatic thiols with good selectivity and displayed ∼700-fold fluorescence intensity enhancement and a remarkable limit of detection (4.5 nM). The new probe also can be applied to quantitative determine the concentrations of thiophenol in water samples and living cells. © 2016 American Chemical Society.
语种：
英文
展开
