Molecular characterization of a peptidoglycan recognition protein from the cotton bollworm, Helicoverpa armigera and its role in the prophenoloxidase activation pathway
作者：
Li, Yu-Ping;Xiao, Min;Li, Li;Song, Cai-Xia;Wang, Jia-Lin;...
期刊：
Molecular Immunology ,2015年65(1):123-132 ISSN：0161-5890
通讯作者：
Liu, Xu-Sheng
作者机构：
[Liu, Xu-Sheng; Li, Li; Song, Cai-Xia; Wang, Jia-Lin; Li, Yu-Ping; Xiao, Min] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Liu, Xu-Sheng] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Xu-Sheng] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, 152 Luoyu Rd, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;Melanization;Pattern recognition receptor;Peptidoglycan recognition protein;Prophenoloxidase activation
摘要：
Peptidoglycan recognition proteins (PGRPs), which are evolutionarily conserved from invertebrates to vertebrates, function as pattern-recognition and effector molecules in innate immunity. In this study, a PGRP (HaPGRP-A) from the cotton bollworm, Helicoverpa armigera was identified and characterized. Sequence analysis indicated that HaPGRP-A is not an amidase-type PGRP. Increased levels of HaPGRP-A mRNA were observed in the fat body and hemocytes of H. armigera larvae following the injection of microbes or Sephadex beads. Analysis using purified recombinant HaPGRP-A showed that it (i) could bind and agglutinate Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus, (ii) enhanced prophenoloxidase activation in the presence of microbes, (iii) promoted the formation of melanotic nodules in vivo, and (iv) enhanced the melanization of Sephadex beads in vivo. RNA interference assays were performed to further confirm the function of HaPGRP-A. When the expression of HaPGRP-A in H. armigera larvae was inhibited by dsHaPGRP-A injection, the phenoloxidase activity in larval hemolymph was significantly decreased and RNAi-treated insects infected with bacteria showed higher bacterial growth in hemolymph compared with infected control larvae. These results indicated that HaPGRP-A acts as a pattern recognition receptor and binds to the invading organism to trigger the prophenoloxidase activation pathway of H. armigera, and the activated phenoloxidase may participate in the melanization process of nodulation and encapsulation responses. Copyright © 2015 Elsevier Ltd. All rights reserved.
语种：
英文
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蜕皮激素调控棉铃虫体液免疫
作者：
汪家林;陈蕾;唐林;赵浩斌;刘绪生
（ ;...
作者机构：
华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉 430079
会议名称：
中国昆虫学会2014年学术年会暨学会成立70周年纪念会
会议时间：
2014-10-01
会议地点：
河北保定
会议论文集名称：
中国昆虫学会2014年学术年会暨学会成立70周年纪念会论文集
关键词：
棉铃虫;转录组;数字基因表达谱;脂肪体;体液免疫;20-羟基蜕皮酮
摘要：
当完全变态昆虫处于wandering stage时,其血腔内20-羟基蜕皮酮(20E)的滴度会上升,并会影响昆虫的体液免疫.然而,20E对昆虫的体液免疫究竟是起着激活作用还是抑制作用尚需进一步研究.我们利用高通量测序技术分析了经肽聚糖刺激后的棉铃虫(Helicoverpa armigera)脂肪体转录组,获得了32,073个unigenes,平均长度为643nucleotides.
语种：
中文
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SRP gene is required for Helicoverpa armigera prophenoloxidase activation and nodulation response
作者：
Qiao, Chuan;Li, Jie;Wei, Xiu-Hong;Wang, Jia-Lin;Wang, Yu-Feng;...
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2014年44(1):94-99 ISSN：0145-305X
作者机构：
[Liu, Xu-Sheng; Li, Jie; Wang, Jia-Lin; Qiao, Chuan; Wei, Xiu-Hong; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Xu-Sheng] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;SRP;Prophenoloxidase activation;Nodulation;Innate immunity
摘要：
SRP gene was first identified from the fall webworm, Hyphantria cunea as one of genes up-regulated after bacteria injection. A rent study in Spodoptera litura showed that stress-induced elevation of SRP expression highly correlates with reduced feeding activities and growth retardation of larvae. In this study, we identified a SRP gene from the cotton bollworm, Helicoverpa armigera, namely Ha-SRP, and studied its precise roles in insect immunity. Expressions of Ha-SRP were upregulated in H. armigera larval hemocytes after injection of Escherichia coli. When the expression of Ha-SRP in H. armigera larval hemocytes was inhibited by dsHa-SRP injection, the transcription of prophenoloxidase genes in hemocytes was repressed, phenoloxidase activity in bacteria-challenged larval hemolymph was significantly decreased, and nodule formation in bacteria-injected larvae was reduced. More importantly, RNAi-treated insects infected with E. coli showed higher bacterial growth in hemolymph compared with infected controls. These results suggest that Ha-SRP gene plays importance roles in H. armigera innate immunity, possibly by mediating prophenoloxidase activation and nodulation response.
语种：
英文
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Involvement of a pattern recognition receptor C-type lectin 7 in enhancing cellular encapsulation and melanization due to its carboxyl-terminal CRD domain in the cotton bollworm, Helicoverpa armigera
作者：
Wang, Jia-Lin
* （ ;Zhang, Qi;Tang, Lin;Chen, Lei;Liu, Xu-Sheng;...
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2014年44(1):21-29 ISSN：0145-305X
作者机构：
[Wang, Jia-Lin; Zhang, Qi; Tang, Lin; Chen, Lei; Liu, Xu-Sheng; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Jia-Lin] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
C-type lectin;CRD;Encapsulation;Melanization;Helicoverpa armigera
摘要：
C-type lectins play important roles in innate immunity as pattern recognition receptors (PRRs). We have previously reported a novel C-type lectin HaCTL7 from the cotton bollworm (Helicoverpa armigera) which contains two carbohydrate-recognition domains (CRDs), namely N-terminal CRD1 and C-terminal CRD2. Interestingly, there are four but not six of conserved cysteine residues in CRD2 of HaCTL7, which is different from that of other dual CRD C-type lectins. In the current study, we expressed and purified recombinant HaCTL7 (rHaCTL7) as well as rCRD1 and rCRD2, and demonstrated that both rHaCTL7 and rCRD2, but not rCRD1, owned the agglutinate ability against both Gram-negative and Gram-positive bacteria in a calcium dependent manner. In addition, both rHaCTL7 and rCRD2, but not rCRD1, could bind to various bacteria, and enhanced haemocytes mediated encapsulation and melanization processes. HaCTL7 secreted from fat bodies is able to bind to granulocytes, plasmatocytes and oenocytoids, but not to spherulocytes. Recombinant HaCTL7 and rCRD2 are capable of binding to both granulocytes and oenocytoids, while rCRD1 can only bind to granulocytes. Our data suggest that as a PRR HaCTL7 enhances encapsulation and melanization likely through its C-terminal CRD2, but not N-terminal CRD1, which imply that the characteristic four cysteine structure of CRD2 plays key roles in innate immunity. © 2013 Elsevier Ltd.
语种：
英文
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棉铃虫DFP1基因在血细胞吞噬作用中的功能分析
作者：
魏秀红;李玉苹;李杰;李丽;汪家林;...
作者机构：
华中师范大学生命科学学院,武汉 430079
会议名称：
中国昆虫学会2014年学术年会暨学会成立70周年纪念会
会议时间：
2014-10-01
会议地点：
河北保定
会议论文集名称：
中国昆虫学会2014年学术年会暨学会成立70周年纪念会论文集
关键词：
棉铃虫;先天免疫;包囊作用;血细胞
摘要：
[目的]前期从棉铃虫血细胞转录组中鉴定到一个免疫相关基因,命名为defense protein1(DFP1).本文研究了DFP1基因在棉铃虫先天免疫中的具体功能.[方法]利用qRT-PCR、重组蛋白体外功能分析、Western blotting、RNAi等方法研究了DFP1基因在棉铃虫血细胞包囊作用中的作用方式.
语种：
中文
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Identification and functional characterization of a peptidoglycan recognition protein from the cotton bollworm, helicoverpa armigera
作者：
Li, Li;Li, Yu-Ping;Song, Cai-Xia;Xiao, Min;Wang, Jia-Lin;...
期刊：
Archives of Insect Biochemistry and Physiology ,2014年86(4):240-258 ISSN：0739-4462
作者机构：
[Liu, Xu-Sheng; Li, Li; Song, Cai-Xia; Wang, Jia-Lin; Li, Yu-Ping; Xiao, Min] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, Xu-Sheng] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
peptidoglycan recognition protein;Helicoverpa armigera;encapsulation;phagocytosis
摘要：
Peptidoglycan recognition proteins (PGRPs) specifically bind to peptidoglycans, and play crucial roles as pattern recognition receptors (PRRs) in mediating innate immune responses. In this study, we identified and characterized a PGRP (HaPGRP-D) from the cotton bollworm, Helicoverpa armigera. Sequence analysis indicated that HaPGRP-D is an amidase-type PGRP. Expression of HaPGRP-D was upregulated in the hemocytes of H. armigera larvae after injecting Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus, or chromatography beads. To test the biological activity of HaPGRP-D, purified recombinant protein was prepared. Subsequent analysis showed that rHaPGRP-D (i) could bind and agglutinate Gram-negative E. coli and Gram-positive S. aureus in a zinc-dependent manner, (ii) functioned as an amidase to degrade peptidoglycans in the presence of Zn 2+ , (iii) strongly inhibited the growth of E. coli and S. aureus in the presence of Zn 2+ , (iv) could bind to the surface of hemocytes, (v) increased the phagocytosis of E. coli cells by hemocytes in vitro, and (vi) promoted hemocyte encapsulation on chromatography beads in vitro. These results suggest that HaPGRP-D plays important roles as PRR, amidase, and opsonin in H. armigera humoral and cellular immune responses. © 2014 Wiley Periodicals, Inc.
语种：
英文
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20-hydroxyecdysone transcriptionally regulates humoral immunity in the fat body of Helicoverpa armigera
作者：
Wang, J-L.;Chen, L.;Tang, L.;Zhao, H-B.;Liu, X-S.
* （ ;...
期刊：
Insect Molecular Biology ,2014年23(6):842-856 ISSN：0962-1075
作者机构：
[Liu, X-S.; Wang, Y-F.; Chen, L.; Tang, L.; Wang, J-L.; Zhao, H-B.] Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Liu, X-S.] C;Cent China Normal Univ, Sch Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;transcriptome;digital gene expression (DGE);fat body;immunity;20-hydroxyecdysone (20E)
摘要：
20-hydroxyecdysone (20E) increases its titre level during the wandering stage and influences innate immunity in many holometabolous insects. However, the function of 20E as an immune-activator or -suppressor needs to be determined. Here, the transcriptome of the peptidoglycan-challenged fat body of the cotton bollworm, Helicoverpa armigera, was analysed using Illumina sequencing technology. Overall, 32 073 unigenes were assembled with a mean length of 643 nucleotides. Gene expression dynamics in the fat body during the wandering stage and of peptidoglycan-challenged individuals were investigated by the digital gene expression system. Pattern recognition receptors [such as peptidoglycan recognition protein B (PGRP B), PGRP S2 precursor, C-type lectin 5, hemolin and β-1,3-glucan recognition protein 2a] and antimicrobial peptides (namely attacin, gloverin, gloverin precursor, gloverin-like, cecropin 2, cecropin D, cecropin D-like and i-type lysozyme) significantly increased their mRNA levels during the wandering stage. 20E treatment significantly induced the expression of these genes. Antibacterial activities were also enhanced during the wandering stage and after 20E injections. Bacillus subtilis peptidoglycan induced the expression of PGRP D, PGRP B, PGRP S2 precursor, gloverin, gloverin precursor, gloverin-like, cecropin 2, cecropin D and lebocin-like genes. These results demonstrate that 20E acts by enhancing humoral immunity in H. armigera. © 2014 The Royal Entomological Society.
语种：
英文
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Cloning and functional analysis of glutathione peroxidase gene in red swamp crayfish Procambarus clarkii
作者：
Xia, Xiao-Fei;Zheng, Jin-Jing;Shao, Guang-Ming;Wang, Jia-Lin;Liu, Xu-Sheng
（ ;...
期刊：
Fish & Shellfish Immunology ,2013年34(6):1587-1595 ISSN：1050-4648
作者机构：
[Liu, Xu-Sheng; Shao, Guang-Ming; Zheng, Jin-Jing; Wang, Jia-Lin; Xia, Xiao-Fei; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Yu-Feng] C;Cent China Normal Univ, Coll Life Sci, 152 Luoyu Ave, Wuhan 430079, Peoples R China.
关键词：
Procambarus clarkii;GPx;Expression patterns;Microbe challenge;ROS
摘要：
Glutathione peroxidases (GPxs) are key enzymes in the antioxidant defense systems of living organisms, including crustaceans. The red swamp crayfish Procambarus clarkii is the most commonly farmed freshwater crayfish in Chinese inland nowadays due to its commercial value. However, high stocking density has resulted in adverse effects in growth performance and health. To investigate the function of GPxs in immune defense of the crayfish, we cloned and characterized a full length GPx (. PcGPx) from P. clarkii by a reverse-transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The 931 bp PcGPx cDNA contains a 38 bp 5'-untranslated region (UTR), a 519 bp coding sequence (CDS) and a 375 bp 3'-UTR with a selenocysteine insertion sequence (SECIS). The PcGPx was predicted to encode 172 amino acids, and its putative molecular mass was 20.9kDa with a pI of 4.37. A selenocysteine (Sec) encoded by the unusual stop codon, TGA, was in the protein coding region. Phylogenetic analysis showed that PcGPx clustered with the GPxs from the penaeid shrimp Metapenaeus ensis and Caenorhabditis elegans, sharing much higher similarity with vertebrate GPx1 and GPx2 than with GPx3 and GPx5. Quantitative RT-PCR revealed that PcGPx was extremely highly expressed in ovary and early embryos. In addition, the levels of PcGPx mRNA and reactive oxygen species (ROS) significantly increased after challenge with gram-negative Vibrio harveyi, gram-positive Staphyloccocus aureus or white spot syndrome virus (WSSV). These results suggest that PcGPx may play important roles not only in immune defense, but also in oogenesis in the crayfish. © 2013 Elsevier Ltd.
语种：
英文
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Functional comparative analysis of two C-type lectins HaCTL3 and HaCTL7 in the cotton bollworm, Helicoverpa armigera
作者：
Jia-Lin Wang;Qi Zhang;Xu-Sheng Liu
（ ;Yu-Feng Wang
作者机构：
[Xu-Sheng Liu; Jia-Lin Wang; Qi Zhang; Yu-Feng Wang] Laboratory of Genetic Regulation and Integrative Biology, College of Life Science,Central China Normal University, Wuhan 430079, China
会议名称：
The 10th Conference of Insect Physiology, Biochemistry and Molecular Biology(第四届国际昆虫生理生化与分子生物学学术研讨会暨第十届全国昆虫生理生化与分子生物学学术会议)
会议时间：
2013-6-15
会议地点：
南京
会议主办单位：
中国昆虫学会
会议论文集名称：
The 10th Conference of Insect Physiology, Biochemistry and Molecular Biology(第四届国际昆虫生理生化与分子生物学学术研讨会暨第十届全国昆虫生理生化与分子生物学学术会议)论文集
关键词：
HaCTL3;HaCTL7;RD;Helicoverpa armigera
摘要：
<正>C-type lectins play important roles in innate immunity as pattern recognition receptors（PRRs）.Previously,we obtained two C-type lectins HaCTL3and HaCTL7 from the cotton bollworm（Helicoverpa armigera）,
语种：
英文
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Identification and characterization of two peptidoglycan recognition proteins with zinc-dependent antibacterial activity from the cotton bollworm, Helicoverpa armigera
作者：
Yang, Dai-Qun;Su, Zhen-Lin;Qiao, Chuan;Zhang, Zan;Wang, Jia-Lin;...
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2013年39(4):343-351 ISSN：0145-305X
作者机构：
[Liu, Xu-Sheng; Yang, Dai-Qun; Wang, Jia-Lin; Qiao, Chuan; Su, Zhen-Lin] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.;[Zhang, Zan; Li, Fei] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China.
通讯机构：
[Liu, Xu-Sheng] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
Helicoverpa armigera;Hormone;Immunity;Peptidoglycan recognition protein
摘要：
Peptidoglycan recognition proteins (PGRPs) specifically bind to peptidoglycan and play an important role in the innate immune responses as pattern recognition receptors (PRRs). Here we identified and characterized two PGRPs (HaPGRP-B and HaPGRP-C) from the cotton bollworm, Helicoverpa armigera. The comparative analysis indicated that five amino acids which are required for T7 lysozyme Zn2+ binding and amidase activity are conserved in HaPGRP-B and HaPGRP-C, suggesting that the two PGRPs are members of the amidase-type PGRPs. HaPGRP-B and HaPGRP-C mRNA increased in both the fat bodies and the hemocytes after an injection of Gram-negative Escherichia coli or Gram-positive Staphylococcus aureus. Recombinant HaPGRP-B and HaPGRP-C could agglutinate E. coli and S. aureus in a zinc-dependent manner. More importantly, both rHaPGRP-B and rHaPGRP strongly inhibited the growth of E. coli and S. aureus in the presence of Zn2+. Moreover, the HaPGRP-B mRNA showed up-regulation post hormones (20E and methoprene) injection. Our results indicate that the two PGRPs of H. armigera may play an important role in defending against bacteria as amidase-type PGRPs and the hormones can function in regulating the expressions of PGRPs. (C) 2013 Elsevier Ltd. All rights reserved.
语种：
英文
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Expression profiles of six novel C-type lectins in response to bacterial and 20E injection in the cotton bollworm (Helicoverpa armigera)
作者：
Wang, Jia-Lin
* （ ;Liu, Xu-Sheng;Zhang, Qi;Zhao, Hao-Bin;Wang, Yu-Feng
期刊：
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY ,2012年37(2):221-232 ISSN：0145-305X
作者机构：
[Wang, Jia-Lin; Zhang, Qi; Liu, Xu-Sheng; Zhao, Hao-Bin; Wang, Yu-Feng] Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
通讯机构：
[Wang, Jia-Lin] C;Cent China Normal Univ, Coll Life Sci, Hubei Key Lab Genet Regulat & Integrat Biol, Wuhan 430079, Peoples R China.
关键词：
20E;C-type lectin;Helicoverpa armigera;Immunity
摘要：
C-type lectins can act as pattern recognition receptors (PRRs) and play an important role in innate immunity. Two C-type lectins (HaCTL1 and HaCTL2) have been previously identified in the cotton bollworm (. Helicoverpa armigera). Here we isolate six C-type lectins from . H. armigera (HaCTL3, 4, 5, 6, 7 and 8). All six new HaCTLs encode a signal peptide (or partial signal peptide) and complete tandem carbohydrate-recognition domains (CRDs). . HaCTL4, . 5, . 6, . 7 and . 8 mRNA increased in the fat body after injection with both killed Gram-negative . Escherichia coli and Gram-positive . Staphylococcus aureus, whereas . HaCTL3 mRNA was upregulated following . E. coli injection only. Recombinant HaCTL3 exhibited agglutinating activity against both Gram-negative and Gram-positive bacteria in a calcium-dependent manner. Agglutination inhibitory analysis indicated that rHaCTL3 recognizes maltose, trehalose, peptidoglycan and lipopolysaccharides. . HaCTL3 and . HaCTL8 mRNA showed upregulation while . HaCTL4, . 5, and . 6 mRNA downregulation post 20-Hydroxyecdysone (20E) injection. Our results indicate that the six novel C-type lectins of . H. armigera may play important roles in defending against bacteria as PRRs and the hormone 20E can function in regulating immunity through lectins. © 2012 Elsevier Ltd.
语种：
英文
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微卫星标记和线粒体细胞色素b基因检测犬蝠偏性扩散行为
作者机构：
[刘绪生; 孙艳红; Monagin C; 陈金平] 广东省昆虫研究所;[刘绪生; 孙艳红; Monagin C; 陈金平] 华南濒危动物研究所;[刘绪生; 孙艳红; Monagin C; 陈金平] Global Virus Forecasting Initiative;[刘绪生; 孙艳红; Monagin C; 陈金平] 华中师范大学生命科学院
会议名称：
第七届全国野生动物生态与资源保护研讨会
会议时间：
2011-10-28
会议地点：
浙江金华
会议主办单位：
中国生态学学会;中国动物学会
会议论文集名称：
第七届全国野生动物生态与资源保护研讨会论文集
摘要：
<正>动物的许多行为方式由于雌雄性别而表现出差异。扩散是动物普遍具有的一种行为方式,是维持种群延续和新种形成的因素之一,通常也表现出雌雄性别差异。偏性扩散在陆地兽类和鸟类中研究较多,而作为唯一能飞翔的兽类蝙蝠的扩散行为的研究则少有报道。犬蝠(Cynopterus
语种：
中文
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中华卵索线虫vasa基因的克隆及其表达模式分析
期刊：
植物保护学报 ,2011年38(4):333-338 ISSN：0577-7518
作者机构：
[刘绪生; 任爽; 陈冲; 王国秀] 华中师范大学生命科学学院
关键词：
昆虫病原线虫;中华卵索线虫;vasa基因克隆;表达分析
摘要：
为明确vasa基因在中华卵索线虫Ovomermis sinensis生殖系发育以及原始生殖细胞增殖过程中的作用,采用同源克隆与cDNA末端快速扩增技术（RACE）,从中华卵索线虫中克隆得到vasa基因全长cDNA序列（3053bp）,编码813个氨基酸,具有DEAD-box家族蛋白全部9个保守基序。经同源比对与系统进化树分析,确定其属于DEAD-box家族的VASA亚家族成员。利用反转录PCR（reverse transcription PCR,RT-PCR）以及实时荧光定量PCR（real time PCR）分析,vasamRNA在中华卵索线虫性腺中专一性表达,其它部位如头、尾、体壁均未检测到vasa表达信号,且在生殖腺发育初期表达量最高。此外,vasa基因转录本在中华卵索线虫胚胎发育期卵中表达量较高,证实其为母源性基因,调控中华卵索线虫胚胎发育的过程。
语种：
中文
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棉铃虫血细胞包囊作用相关基因的鉴定及功能研究
项目作者单位：
华中师范大学
项目批准号：
31000982
资助经费：
21万
立项时间：
2011-01-01到2013-12-31
项目类别：
青年科学基金项目
项目来源：
国家自科基金项目
项目关键词：
棉铃虫;包囊作用;细胞免疫;先天性免疫
项目摘要：
目前关于昆虫细胞免疫特别是血细胞包囊作用的分子机制还不很清楚。本项研究拟以棉铃虫作为研究对象，应用抑制消减杂交(SSH)技术，构建棉铃虫经葡聚糖凝胶珠免疫后的差异表达基因谱；通过对这些差异表达基因的分析,选择一些可能与包囊作用相关的候选基因，利用原位杂交、免疫组化、荧光定量PCR、western杂交、体外包囊分析等方法研究这些候选基因在棉铃虫血细胞包囊作用中的功能（在包囊作用的哪个阶段参与调控；可能在包囊作用的哪个环节参与调控等）。本项目的完成将鉴定一些棉铃虫血细胞包囊作用相关基因,初步了解棉铃虫血细胞包囊作用的分子机理，加深人们对昆虫先天性免疫分子机制的认识。
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湖北省爬行动物资源概况
期刊：
动物学杂志 ,2011年46(5):132-139 ISSN：0250-3263
作者机构：
华中师范大学生命科学学院生态与进化生物学实验室 武汉430079;[刘绪生; 戴宗兴; 戴琦; 周权] 华中师范大学
关键词：
湖北省;爬行动物;资源;区系
摘要：
为了搞清湖北省爬行动物资源概况,通过总结文献资料,并结合作者等最近20余年积累的调查资料,从区系特点、地理分布、评估等级、资源量及保护对策等方面总结了湖北省爬行动物的资源概况。结果显示,湖北省现有爬行动物2目12科44属78种,主要分布在鄂西南（57种,占73.1%）、鄂西北（47种,占60.3%）和鄂东南（43种,占55.1%）地区。东洋界种62种,占79.5%,古北界种8种,占10.3%,广布种8种,占10.3%。东洋界种中,华中华南区成分36种,占58.1%;华中区成分15种,占24.2%;西南区成分11种,占17.7%。列入《中国物种红色名录》25种,占32.1%;省级重点保护动物16种,占20.5%。湖北省爬行动物资源具有以东洋界华中华南区种类为主,省内各区分布不均,濒危保护种类丰富等特点。
语种：
中文
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湖北省两栖动物资源概况
期刊：
四川动物 ,2011年30(1):144-147 ISSN：1000-7083
作者机构：
[刘绪生; 戴宗兴; 张洪茂; 周权] 华中师范大学生命科学学院, 生态学与进化生物学实验室
关键词：
湖北省;两栖动物;资源;区系
摘要：
通过总结文献资料并结合作者等近20余年调查积累的资料, 从区系特点、地理分布、保护级别等方面总结了湖北省两栖动物的资源概况. 湖北省现有两栖动物2目10科28属69种, 其中东洋界61种, 占88.4％, 古北界4种, 占5.8％, 广布种4种, 占5.8％; 国家Ⅱ保护动物3种, 即大鲵Andrias davidianus、细痣疣螈Tylototriton asperrimus、虎纹蛙Hoplobatrachus rugulosus, 占4.3％; 列入<<中国濒危动物红皮书>>的珍稀濒危物种8种, 占11.6％; 省级重点保护动物23种, 占33.3％. 可见湖北省两栖动物资源具有以东洋界华中区种类为主、省内各区分布不均、濒危保护种类丰富等特点
语种：
中文
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湖北省发现中国瘰螈
作者：
苏振林;曹国斌;朱兆泉;周权;刘绪生
（ ;...
期刊：
动物学杂志 ,2010年45(5):164-165 ISSN：0250-3263
作者机构：
[刘绪生; 苏振林; 周权; 戴宗兴] 华中师范大学生命科学学院;[曹国斌; 朱兆泉] 湖北省野生动植物保护总站
关键词：
中国瘰螈;新纪录;湖北省
摘要：
2009年6～9月,在咸丰县丁寨乡、甲马池乡采集到一批两栖类标本,经鉴定其中中国瘰螈（Paramesotriton chinesis）为湖北省新纪录种。
语种：
中文
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湖北星斗山国家级自然保护区两栖爬行动物资源调查
作者：
李神斌;苏振林;童萍;刘国跃;刘绪生
（ ;...
期刊：
四川动物 ,2010年29(1):130-133 ISSN：1000-7083
作者机构：
[刘绪生; 李神斌; 苏振林; 戴宗兴] 华中师范大学生命科学学院;[童萍] 宜昌市第二中学;[刘国跃] 武汉动物园
关键词：
星斗山国家级自然保护区;两栖爬行动物;资源;保护对策
摘要：
2002年6～9月对湖北星斗山国家级自然保护区的两栖、爬行动物资源进行了调查,结合文献资料,该保护区现已知有两栖、爬行动物80种,其中两栖动物38种,隶属2目9科22属;爬行动物42种,隶属2目11科31属.国家Ⅱ级重点保护野生动物大鲵Andrias davidianus、虎纹蛙Hoplobatrachus rugulosus 2种;湖北省重点保护动物35种,占湖北省重点保护两栖爬行动物的75%.被列入<中国濒危动物红皮书>的两栖、爬行动物有21种(极危物种1种,濒危物种6种,易危物种11种,依赖保护物种1种,需予关注物种2种).对两栖爬行动物的种类组成和区系进行了分析,并提出了保护措施.
语种：
中文
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Contrasting Genetic Structure in Two Co-Distributed Species of Old World Fruit Bat
作者：
Chen, Jinping
* （ ;Rossiter, Stephen J.;Flanders, Jonathan R.;Sun, Yanhong;Hua, Panyu;...
期刊：
PLOS ONE ,2010年5(11):e13903- ISSN：1932-6203
作者机构：
[Chen, Jinping; Sun, Yanhong] Guangdong Entomol Inst, Guangzhou, Guangdong, Peoples R China.;[Rossiter, Stephen J.] Queen Mary Univ London, Sch Biol & Chem Sci, London, England.;[Flanders, Jonathan R.] Univ Bristol, Sch Biol Sci, Bristol, Avon, England.;[Sun, Yanhong; Liu, Xusheng] Huazhong Normal Univ, Wuhan, Peoples R China.;[Zhang, Shuyi; Hua, Panyu] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China.
通讯机构：
[Chen, Jinping] G;Guangdong Entomol Inst, Guangzhou, Guangdong, Peoples R China.
关键词：
Phylogeography;Haplotypes;Population genetics;China;India;Bats;Fruit bats;Gene flow
摘要：
The fulvous fruit bat (Rousettus leschenaulti) and the greater short-nosed fruit bat (Cynopterus sphinx) are two abundant and widely co-distributed Old World fruit bats in Southeast and East Asia. The former species forms large colonies in caves while the latter roots in small groups in trees. To test whether these differences in social organization and roosting ecology are associated with contrasting patterns of gene flow, we used mtDNA and nuclear loci to characterize population genetic subdivision and phylogeographic histories in both species sampled from China, Vietnam and India. Our analyses from R. leschenaulti using both types of marker revealed little evidence of genetic structure across the study region. On the other hand, C. sphinx showed significant genetic mtDNA differentiation between the samples from India compared with China and Vietnam, as well as greater structuring of microsatellite genotypes within China. Demographic analyses indicated signatures of past rapid population expansion in both taxa, with more recent demographic growth in C. sphinx. Therefore, the relative genetic homogeneity in R. leschenaulti is unlikely to reflect past events. Instead we suggest that the absence of substructure in R. leschenaulti is a consequence of higher levels of gene flow among colonies, and that greater vagility in this species is an adaptation associated with cave roosting.
语种：
英文
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棉铃虫血细胞对葡聚糖凝胶珠的包囊反应
作者：
郭旭;苏振林;钟淑芳;袁媛;刘绪生
（ 期刊：
应用昆虫学报 ,2010年47(4):744-747 ISSN：2095-1353
作者机构：
[刘绪生; 郭旭; 苏振林; 钟淑芳; 袁媛] 华中师范大学生命科学学院
关键词：
棉铃虫;包囊作用;血细胞;葡聚糖凝胶珠
摘要：
研究了棉铃虫Helicoverpa armigera(Hübner)血细胞对葡聚糖凝胶珠的包囊反应. 结果表明:棉铃虫血细胞对不同类型的葡聚糖凝胶珠具有不同的包囊能力, 其中对Sephadex A-25葡聚糖凝胶珠具有很强的包囊能力;棉铃虫血细胞启动对Sephadex A-25葡聚糖凝胶珠的包囊反应是较迅速的, 而包囊的完成大概是在凝胶珠进入棉铃虫体内12 h之后;在离体的情况下, 棉铃虫血细胞仍然能包囊葡聚糖凝胶珠
语种：
中文
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