Dipacus asperoides (DA), a traditional perennial herb, has distributed wildly in China, especially Wuhe Xuduan known as genuine herb. To evaluate DA's quality, we collected ten samples from eight counties in Enshi and determined the contents of total saponins (TS) and asperosaponin VI (Asp. VI) using vanillin - glacial acetic acid - perchloric acid with spectrophotometry and ultra-high performance liquid chromatography (UPLC) separately. Subsequently, we carried out the optimization extraction of TS, Asp. VI and several organic acids such as lo...
