We thank Dr Ming Sun and Hubei Bt Center for providing us with plasmid expressing ICPs and Bt formula. We also thank Prof Quhou Chen for giving us helpful suggestions. This work was supported by a grant from Natural Science Foundation of P. R. China (30571249).
机构署名：
本校为第一且通讯机构
院系归属：
生命科学学院
摘要：
Cabbage looper moth (Trichoplusia ni) cell line BTI-Tn-5B1-4 (TnH5) has developed high-level resistance (>1000 fold) by the selection of Bt Cry1Ac10 toxin. In order to examine mechanisms of resistance to Cry1Ac10 toxin (biological pesticide), both general esterase activities and cell tolerance to osmotic lysis were compared between non-selected Cry1Ac10-susceptible Trichoplusia ni cell line TnH5-S and Cry1Ac10-resistant Trichoplusia ni cell line TnH5-R selected by Bt Cry1Ac10. The Cry1Ac10-resistant TnH5-R cells had lower general esterase activ...
