期刊论文

Effects of Chemical Factors on Formation of Cyclosporins by Submerged Cultures of Tolypocladiun inflatum

中文

应用基础与工程科学学报

Journal of Basic Science and Engineering

1005-0930

1997

37

03

243-251

本校为其他机构

发酵培养物中加入亚硫酸氢钠,可提高环孢菌素A生产率。在发酵的前期(0～3d)亚硫酸氢钠对细胞中蛋白质的形成有明显促进作用,而在发酵的后期(3～13d)亚硫酸氢钠不利于细胞中蛋白质的形成。德夸菌素(Decoyinine)加到发酵第5天的培养物中,可提高环孢菌素的形成150%,提高环孢菌素A的生产率370%。经过黄体酮(细胞色素诱导剂)处理的雪状白僵菌接种到不含黄体酮的发酵培养基中,直接地影响了环孢菌素的生产率,而且也改变了个别成分在混合物中所占的比例。

Sodium bisulfite intervention resulted in an enhanced specific production of cyclosporin A. In early fermentation (0～3d) sodium bisulfite increased the level of cell protein, whereas in the consequent period(3～13d) the effect of chemical on the level of cell proteins was negative. Deeoyinine-inhibitor of guanosine-5'-monophosphate synthetase when added to the 5d old culture increased the sporulation intensity by 150%, the specific production was enhanced hy 370% (8d). Inoculum of T. inflatum supplemented with. progesterone (inducer of cytochr...