Peroxynitrite (ONOO − ) is one of the most important reactive oxygen species (ROS) in living systems and its subcellular detection is very important. In this research, a lysosome-targetable fluorescent probe for detection of exogenous and endogenous peroxynitrite in living systems was developed. This probe exhibits not only fast (25 s), highly sensitive (detection limit = 16 nM), and highly selective red to near-infrared fluorescent turn-on responses for ONOO − in the solution, but also excellent imaging ability for ONOO − in living cells an...
