Glycosylation modification plays a vital role in tumor progression and is highly associated with glioma prognosis. However, the influence of glycosylation modification on the tumor microenvironment (TME) and omic features of glioma remains unclear. Differentially expressed glycosylation-related genes between adjacent and tumor tissues of The Cancer Genome Atlas and Chinese Glioma Genome Atlas datasets were identified. We performed unsupervised clustering to classify patients into different molecular phenotypes, and analyzed their TME heterogene...