This study examines the binaural and frequency representation in the primary auditory cortex (AC) of the big brown bat, Eptesicus fuscus, by using an ear-phone stimulation system. All 306 cortical neurons studied were excited by contralateral sound stimulation but they were either excited, inhibited or not affected by ipsilateral sound stimulation. These cortical neurons were columnarly organized according to their binaural and frequency-tuning properties. The excitation-excitation columns which occupy about 15% of the AC are mainly aggregated within an oval-shaped area of the central AC. The ...