A near-infrared (NIR) ratiometric fluorescent probe, NIR-Ratio-CO, was developed for rapid detection of carbon monoxide (CO) in both solution and living cells through the strategy of Pd(0)-mediated Tsuji-Trost reaction. This probe shows a rapid, highly specific and sensitive detection process for CO, accompanied by colorimetric and distinct ratiometric fluorescence changes at 655 and 592nm with a large Stokes shift up to 195nm. The detection limit for CO was measured to be about 61nM by the fluorescence method. In addition, this probe was successfully applied for ratiometric imaging of both ex...
