期刊论文

CHARACTERISTICS OF HYDROCARBON GENERATION FROM HYDROUS PYROLYSIS OF FANZHI LIGNITE

中文

石油大学学报. 自然科学版

Journal of the University of Petroleum,China(Edition of Natural Science)

1000-5870

1999

18

2

71-74

10.3321/j.issn:1000-5870.1999.02.020

国家自然科学基金

本校为第一机构

PrfA是单核细胞增生李斯特菌(L.monocytogenes)中迄今为止发现的唯一一个调控绝大多数毒力基因转录表达的蛋白因子.为了深入研究PrfA结构和功能的关系,模拟一株自然界分离得到的PrfA组成型突变株P14A(血清型4b),将标准野生株EGDe(血清型1/2a)的PrfA第145位甘氨酸突变为丝氨酸(简称PrfA*),分别构建野生型PrfA和突变型PrfA*表达融合载体,提取并纯化相应蛋白用于体外转录,直接检测依赖于PrfA的毒力基因plcA,hly和actA启动子的转录活性;同时,将携带prfA和prfA*的载体分别电转化入prfA基因缺失菌株中,应用实时RT-PCR检测plcA,hly和actA体内转录水平.实验结果显示:突变型PrfA*蛋白明显增强了plcA,hly和...

The low evolution process of Fanzhi lignite was studied by using hydrous pyrolysis reactor. The effects of Na 2CO 3 alkaline solution on the generation of gaseous and liquid products from lignite are assessed. The characteristics and kinetics of product generation were discussed as well. Na 2CO 3 solution promotes the formation of gaseous products and thermal bitumen as an intermediate, but has no effect on the generation of oil from bitumen. The Na 2CO 3 solution, as a strong electrolyte, intensifies the catalysis of hydrous media on kerogen degradation, which can be t...