[Zhang, Zhi-Ming; Zhang, Ru-Yan; Feng, Ran-Ran; Ding, Dong; Wen, Yu; Guo, Jun; Zhou, Shi-Hao] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol &, Hubei Int Sci & Technol Cooperat Base Pesticide &, Wuhan 430079, Peoples R China.
[Wei, Hua-Wei] Jiangsu East Mab Biomed Technol Co Ltd, Nantong 226499, Peoples R China.
通讯机构：
[Jun Guo] K
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, International Joint Research Center for Intelligent Biosensing Technology and Health, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, College of Chemistry, Central China Normal University, Wuhan 430079, China
National Natural Science Foundation of China [22177035, 21772056]; Wuhan Bureau of Science and Technology [2020020601012217]; Fundamental Research Funds for the Central Universities [CCNU22JC007]; Program of Introducing Talents of Discipline to Universities of China (111 program) [B17019]
机构署名：
本校为第一机构
院系归属：
化学学院
摘要：
Modification of antigens to improve their immunogenicity represents a promising direction for the development of protein vaccine. Here, we designed facilely prepared adjuvant-free vaccines in which the N-glycan of SARS-CoV-2 receptor-binding domain (RBD) glycoprotein was oxidized by sodium periodate. This strategy only minimally modifies the glycans and does not interfere with the epitope peptides. The RBD glycoprotein oxidized by high concentrations of periodate (RBD(HO)) significantly enhanced antigen uptake mediated by scavenger receptors and promoted the activation of antigen-presenting ce...
