Rice blast, caused by the ascomycete fungus Magnaporthe grisea, is one of the most serious diseases for cultivated rice. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis in biological kingdoms and an important drug target for microbial pathogenic infections. In the present study CYP51 with truncation of N-terminal residues from M. grisea (MGCYP51) was cloned and expressed in E. coli, drug binding spectrum of MGCYP51 induced by addition of diniconazole was determined. In order to exploit more selective and effecti...