β-actin is a member of the actin family, which plays important roles in maintaining cytoskeleton, cell motility, cell division, intracellular movements and contractile processes. The partial sequence of β-actin cDNA of Gobiocypris rarus was cloned in present work from the muscles by reverse transcription-polymerase chain reaction (RTPCR). This sequence consists of 1029 base pairs which encodes 343 amino acids. The amino acid identities of the corresponding partial sequences of the gene between the Gobiocypris rarus and other fishes were ver...