期刊论文

Liu, D.

中文

生物化学与生物物理进展

1000-3282

1997

24

2

147-150

本校为第一且通讯机构

纯化的重组缣孢菌细胞色素P 4 50nor（recombinantfusariumoxysporumcytochromeP 4 50nor ,rF·P 4 50nor）用于动力学研究 .测得米氏常数Km （NO）和Km （NADH）分别为 0 1 2 8mmol/L和0 2 0 8mmol/L .Vmax （N2 O）为 1 1 363min- 1.光谱吸收特性研究表明 :rF·P 4 50nor具有典型的血红素蛋白的特性 ,在 4 1 3nm有最大吸收峰 .加入还原剂Na2 S2 O4 时 ,最大吸收峰前移至 4 0 5nm .与CO结合后 ,再加入还原剂Na2 S2 O4 时 ,在 4 50nm处表现最大吸收 .与NO结合后 ,最大吸收峰移至4 30nm附近 .这些光谱特征的变化与天然的F·P 4 50nor完全一致

Purified recombinant Fusarium oxysporum cytochrome P 450nor (rF·P 450nor) was used for spectroscopic and kinetic studies. K m and V max were determined. K m(NO)and K m(NADH) were 0 128 mmol/L and 0 208 mmol/L respectively. V max for N 2O was 11 363 min -1 . The spectroscopic study indicated that: rF·P 450nor has the typical characteristics of a heme protein, and has the maximum absorbance at 413 nm. After added Na 2S 2O 4,the maximum absorption peak was moved to 405 nm. After added CO and Na 2S 2O 4, the absorption peak was at 450 nm. When co...