Overexpression of a Cotton Gene That Encodes a Putative Transcription Factor of AP2/EREBP Family in Arabidopsis Affects Growth and Development of Transgenic Plants
National Natural Sciences Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31171174]; Ministry of Agriculture of ChinaMinistry of Agriculture, China [2013ZX08009003-004]; Scientific Research Foundation of Hubei Province [2011CDA140, 2012FFA126]
机构署名：
本校为第一且通讯机构
院系归属：
生命科学学院
摘要：
In the study, a gene encoding a putative ethylene response factor of AP2/EREBP family was isolated from cotton (Gossypium hirsutum) and designated as GhERF12. Sequence alignment showed that GhERF12 protein contains a central AP2/ERF domain (58 amino acids) with two functional conserved amino acid residues (ala14 and asp19). Transactivation assay indicated that GhERF12 displayed strong transcription activation activity in yeast cells, suggesting that this protein may be a transcriptional activator in cotton. Quantitative RT-PCR analysis showed that GhERF12 expression in cotton was induced by AC...
