期刊论文

Preliminary study on the characteristics of superoxide dismutase in Trifolium repens

中文

湖北农业科学

0439-8114

1996

5

41-45

10.14088/j.cnki.issn0439-8114.1996.05.017

国家自然科学基金；

本校为第一机构

白三叶叶片超氧化物歧化酶（ＳＯＤ）粗酶提取液中既含有ＣｕＺｎＳＯＤ，又含有ＭｎＳＯＤ；其同工酶谱带共有５条，其中迁移率为０７１８７的第４条谱带是ＭｎＳＯＤ，其余都是ＣｕＺｎＳＯＤ。粗酶液具有较高的热稳定性，水浴中保温１５ｍｉｎ处理时，活性半抑制温度约为６３℃；粗酶液在恒温水浴中保温处理，６０℃条件下的活性半衰期约为３７７ｍｉｎ。在变性剂ＳＤＳ作用下，第１，２条ＣｕＺｎＳＯＤ活性谱带与第４条ＭｎＳＯＤ活性谱带活力丧失，而第３，５条ＣｕＺｎＳＯＤ活性谱带活性变化较小；在β巯基乙醇作用下，第１，２，３条ＣｕＺｎＳＯＤ活性谱带全部...

A study was made to determine some characteristics of superoxide dismutase(SOD) in white clover(Trifolium repens L. cv. Louisiana K 57114 ). Both types of CuZn SOD and Mn SOD were detected and five isozyme bands found by polyacrylamide gel electrophoretic analysis in the SOD crude etract of white clover leaves with results showing that, the fourth band of which with the morbility of 0 7187 is Mn SOD and others are CuZn SOD. The test on thermal stability of the SOD crude extract indicated that, when incubated for 15 min in water bath, the cataly...