期刊论文

中文

中国食用菌

1003-8310

1997

4

32-34

10.13629/j.cnki.53-1054.1997.04.024

本校为第一机构

灵芝菌丝体超氧化物歧化酶（ＳＯＤ）同工酶谱带有５条，其中酶带Ⅰ，Ⅳ，Ⅴ为Ｍｎ—ＳＯＤ，酶带Ⅱ，Ⅲ为Ｃｕ—Ｚｎ—ＳＯＤ。Ｃｕ—Ｚｎ—ＳＯＤ在不同浓度的ＫＣＮ作用下，其活力随ＫＣＮ的浓度增大而逐渐丧失，而Ｍｎ—ＳＯＤ不受ＫＣＮ影响仍具催化活性；Ｃｕ—Ｚｎ—ＳＯＤ在氯仿—乙醇的作用下仍具催化活性，而Ｍｎ—ＳＯＤ随氯仿—乙醇的浓度增大而失去活力；ＳＤＳ可使Ｍｎ—ＳＯＤ失去活性，但对Ｃｕ—Ｚｎ—ＳＯＤ无影响；β—巯基乙醇使部分Ｃｕ—Ｚｎ—ＳＯＤ失去活性，但对Ｍｎ—ＳＯＤ无影响。

In five isoenzyme bands of SOD in mycelium of Ganoderma lucidum band Ⅰ,Ⅳ,Ⅴ are Mn-SOD and band Ⅱ,Ⅲ are Cu-Zn-SOD. The activity of Cu-Zn-SOD decreases with the improvement of KCN content and β-mercaptoethanol and it is not effected by chloroform-ethanol and SDS. Meantime, the activity of Mn-SOD decreases with the improvement of chloroform-ethanol and SDS, and it i...