Acknowledgments We are very thankful to Prof. Li Yi in the NRS-Institute Armand-Frappier in Canada and Dr. James Sims in the University of Utah in USA for the refining of our manuscript. This research was supported by the National Natural Science Foundation of China (30600014 & 30770036), Wuhan City Science and Technology Plan (200760423162).
机构署名：
本校为其他机构
院系归属：
生命科学学院
摘要：
Screening interesting biocatalysts directly from soil samples is a more convenient and applicable approach than conventional cultivation-dependent ones. In our present work, a soil-derived metagenomic library containing 24,000 transformants was constructed with an efficient strategy for cloning xylanase genes. A gene encoding the enzyme (XynH) able to hydrolyze xylan was obtained. Similarity analysis revealed that this enzyme is a new member in the family 10 of xylanases. The molecular mass of XynH purified from Escherichia coli was estimated t...
