期刊论文

中文

公共卫生与预防医学

1006-2483

2006

17

2

15-18+21+103

10.3969/j.issn.1006-2483.2006.02.005

国家自然科学基金面上项目（30570799）；

本校为第一机构

目的研究甲醛致小鼠肺DNA-蛋白质的交联(DNA-protein crosslinks,DPC)和致DNA的断裂作用.方法以小鼠肺为材料,经体外染毒后,采用KCl-SDS沉淀法和单细胞凝胶电泳法检测液态甲醛染毒后肺部提取细胞中DNA-蛋白质交联物的含量及DNA的断裂效应.结果 KCl-SDS沉淀法,低浓度的液态甲醛(5μM,25 μM)不能引起DNA-蛋白质的交联(P＞0.05),较高浓度(125 μM,625 μM)可以引起明显的DNA-蛋白质的交联作用(125 μM,P＜0.05;625 μM,P＜0.01).而单细胞凝胶电泳法显示甲醛在低浓度(5 μM,25 μM)时可以引起DNA链的断裂(P＜0.01),在较高浓度(125 μM,625 μM)时可以引起交联作用(P＜0.01).结论甲醛在较高浓度时可...

Objective To study the effect of formaldehyde inducing DNA-protein crosslinks （DPC） and DNA damages in mice lung. Methods Mice lung was taken as experimental material, KCl-SDS assay and comet assay were used to determine the amount of DNA-protein erosslinks and effect of DNA damages induced by liquid formaldehyde in vitro. Results KCl-SDS assay showed that formaldehyde could not cause DPC at lower level （5 μM,25 μM,P〉0.05）, while at higher level（125 μM,P〈0.05;625 μM,P〈0.01） it caused DPC obviously. According to comet assay, it caus...