期刊论文

Study on cellular DPC induced by di （2 ethylhexyl）phthalate in organs of mice

中文

公共卫生与预防医学

1006-2483

2006

17

4

5-9

10.3969/j.issn.1006-2483.2006.04.002

国家自然科学基金面上项目（30570799）；

本校为第一机构

目的 为了探讨邻苯二甲酸二乙基己酯(DEHP)导致生物机体内不同器官DNA-蛋白质的交联(DPC)作用.方法 以采用KCl-SDS沉淀法来检测DEHP体外染毒后昆明纯系小鼠体内肝、脑、睾丸、肺等4个器官的细胞中DNA-蛋白质交联含量.结果 当DEHP的浓度为5μM时,小鼠肝细胞的DPC系数与对照组相比没有发生显著的变化;当DEHP的浓度分别升高到10、20、40和80μM时,DPC系数显著上升,且DPC含量与对照组相比有极显著差异;在脑细胞中,当DEHP的浓度分别为5、10 μM时,能显著诱导DPC的生成(P＜0.05),但是,随着DEHP的浓度升高,DPC的含量与对照组相比没有显著性差异;在睾丸的细胞中,当DEHP的浓度为5 μM时,小鼠睾丸的细胞DP...

Objective In order to study the effects of di （2 ethylhexyl）phthalate （DEHP） induced DNA-protein crosslink （DPC） in mice organs. Methods KCl-SDS method was applied to detect DPC contents in livers, brains, testis and lungs of Kunming mice exposed to DEHP. Results The results showed that at the presence of DEHP as 5 μM, DPC coefficient in mice liver cells were comparative with control group. However, DPC coefficient was significantly increased when DEHP concentrations were up to 10, 20, 40 and 80 μM （compared with control group, P〈0.01...