期刊论文

VR1-mRNA expression in lung of rat induced by formaldehyde

中文

公共卫生与预防医学

1006-2483

2006

17

2

12-14+103

10.3969/j.issn.1006-2483.2006.02.004

国家自然基金面上项目“非过敏原性室内空气污染物诱发过敏性哮喘的分子机理研究”（30570799）；

本校为第一机构

目的从分子水平研究中等浓度气态甲醛环境暴露对呼吸系统中类香草素受体（van illo id recep-tor sub type 1,VR 1）基因表达的影响。方法用WH-2型环境气候舱释放的甲醛和净空气,分别通入2只各放有5只幼年大鼠的染毒缸中连续灌流72 h后,迅速取肺,用RT-PCR方法检测大鼠在中等浓度甲醛环境暴露下肺VR 1和管家基因G 3PD表达水平。结果中等浓度甲醛3.0 m g/m3灌流比清洁空气灌流导致幼年大鼠肺VR 1-mRNA表达增加。结论中等浓度甲醛灌流导致幼年大鼠肺部VR 1-mRNA表达量增加。

Objective To investigate the effect of middle-level gaseous formaldehyde on the expression of vanilloid receptor subtype 1 （VR1） gene in the respiratory system, Methods Ten new-born rats were divided into 2 groups, one exposed in the gaseous formaldehyde,the other, in fresh air, for 72 hours. The gases were released from an environmental chamber. The lungs were rapidly dissected from newborn rats after exposure, then the expression level of VR1 and G3PD were determined by RT-PCR. Results The expression of VR1 increased after exposure in 3. 0m...