期刊论文

Study on DNA damage of liquid formaldehyde on brain cells of rat

中文

公共卫生与预防医学

1006-2483

2006

17

6

5-7

10.3969/j.issn.1006-2483.2006.06.002

国家自然基金面上项目（No.30570799） 湖北省科技攻关计捌项目（No.2005AA307801）

本校为第一机构

目的 检测大鼠脑细胞经不同浓度液态甲醛体外染毒后所致的DNA损伤.方法 Wistar大鼠脑细胞暴露于不同浓度液态甲醛(0.005,0.025,0.125和0.625 mmol·L-1)中,染毒1 h,利用单细胞凝胶电泳技术(又称彗星实验)检测脑细胞的DNA损伤.结果 浓度为0.005和0.025 mmol·L-1的甲醛,可造成脑细胞DNA的严重断裂,而0.125和0.625 mmol·L-1的甲醛则引起显著的交联作用.结论 液态甲醛可造成脑组织DNA的断裂和交联.

Objective To explore the DNA damage of brain cells of rat after formaldehyde exposure. Methods Rat brain cells were exposed to different concentrations of liquid formaldehyde （0. 005, 0. 025, 0. 125 and 0. 625 mmol· L^-1） for 1hour. Single cell gel electrophoresis technique （comet assay） was used to test the DNA damage of the brain cells. Results Formaldehyde could significantly cause the break up of the DNA strands at the concentrations of 0. 005 and 0. 025 mmol· L^-1 ,while erosslinks were induced at the level of 0. 125 and 0. 625 mmol·...