期刊论文

Oxidative Damages of Cypermethrin on Mouse Testis Tissues and Antioxidant Role of Vitamin E

中文

环境与职业医学

2095-9982

2013

30

6

465-470

10.13213/j.cnki.jeom.2013.06.017

湖北省教育厅科学技术研究项目（编号:D20122803）；

本校为其他机构

[目的]研究氯氰菊酯(CP)对小鼠睾丸组织的氧化损伤和维生素E的抗氧化作用. [方法]以36只昆明小鼠为受试动物,随机分为6组,包括1个阴性对照组、3个氯氰菊酯染毒组、1个维生素E组和1个高剂量氯氰菊酯(40mg/kg)加维生素E组(100 mg/kg),染毒组按10、20和40 mg/kg 3个剂量水平,维生素E的剂量为100mg/kg,小鼠灌胃染毒7d.取睾丸称重测定脏器系数；以睾丸组织匀浆测定活性氧(ROS)、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量；以睾丸细胞测定DNA-蛋白质交联(DPC)系数；并制作睾丸组织切片,观察其病理损伤. [结果]随着氯氰菊酯染毒剂量的升高,睾丸的脏器系数、ROS、MDA含量和DPC系数逐渐上升,GSH含量逐渐降...

[ Objective ] To investigate the oxidative damages of cypermethrin （CP） on mouse testis tissues and the antioxidant role of vitamin E. [ Methods ] Thirty-six Kuming mice were randomly divided into 6 groups and administered with designed agents by garage for 7d. The groups included 1 negative control group, 3 CP groups （10, 20, and 40mg/kg respectively）, 1 protection group （40mg/kg CP＋ 100mg/kg vitamin E）, and 1 vitamin E group （100mg/kg）. Afterwards, the mice were sacrificed and the testis organ coefficients were calculated. Reactive o...