期刊论文

中文

生理学报

0371-0874

2015

67

5

497-504

10.13294/j.aps.2015.0061

(No.51136002):This work was supported by the Key Project of National Natural Science Foundation of China (No.GRIB201501):and the Open Project Program of Hubei Key Laboratory of Genetic Regulation and Integrative Biology,China

本校为第一机构

本研究旨在探讨甲醛导致机体神经系统病变的具体机制。选用雄性Balb/c小鼠为研究对象,动态吸入甲醛方式染毒7天,每天8 h,甲醛浓度分别为0、0.5、3.0 mg/m~3,同时设置一氧化氮合酶(nitric oxide synthase, NOS)拮抗剂(N~G-monomethyl- L-arginine, L-NMMA)组,该组小鼠同时进行3.0 mg/m~3甲醛染毒。染毒结束后,用试剂盒检测小鼠大脑皮层、海马和脑干中环磷酸腺苷(cyclic adenosine monophosphate, cAMP)、环磷酸鸟苷(cyclic guanine monophosphate, cGMP)、一氧化氮(nitric oxide, NO)含量和NOS活性的变化。结果显示,与对照组相比,小鼠大脑皮层和脑干中cAMP含量在0.5 mg/m~3染毒组显著升高(均P < ...

The aim of this study was to explore the mechanism of the nervous system lesions induced by formaldehyde (FA). Male Balb/c mice were exposed to gaseous formaldehyde for 7 days (8 h/d) with three different concentrations (0, 0.5 and 3.0 mg/m(3)). A group of animals injected with the nitric oxide synthase inhibitor L-NMMA (0.01 mL/g) was also set and exposed to 3.0 mg/m(3) FA. The concentrations of cAMP, cGMP, NO and the activity of NOS in cerebral cortex, hippocampus and brain stem were determined by corresponding assay kits. The results showed that, compared with the control (0 mg/m(3) FA) gro...