期刊论文

Luo, Feng

中文

生理学报

0371-0874

2018

70

2

123-131

10.13294/j.aps.2018.0021

supported by the National Natural Science Foundation of China（No.31300915） the Scientific Research Project Fund for Youth Teachers from Central China Normal University（No.CCNU15A05035）

本校为第一且通讯机构

激活胆碱能受体结合去极化电流诱导产生的持续性放电(persistent activity, PA)是神经元产生可塑性的一种特征表现。小鼠初级听皮层(primary auditory cortex, AI)神经元PA的产生与毒蕈碱受体(即M型受体)密切相关,但其涉及的毒蕈碱受体亚型尚不清楚。本研究采用离体脑片全细胞膜片钳技术结合药理学方法,探讨幼年小鼠AI第V层锥体神经元PA与毒蕈碱受体亚型之间的关系。结果显示,激活胆碱能受体并同时注入去极化电流可诱导锥体神经元产生PA。M1、M2和M3受体拮抗剂分别能阻断PA的产生,而M4受体拮抗剂对PA的产生没有影响。该结果表明M1、M2和M3毒蕈碱受体均参与幼年小鼠AI第V层锥体神经元PA的形成,而...

Cholinergic receptor activation and intracellular current injection lead to the persistent activity (PA), which may be involved in inducing neural plasticity. Our previous study showed that PA is closely related to the activation of muscarinic acetylcholine receptors (mAChRs) in pyramidal neurons of mouse primary auditory cortex (AI). However, the subtypes of mAChRs involved in PA remain unclear. Thus, using whole-cell patch-clamp recording and pharmacological methods, we investigated the role of different mAChR subtypes in inducing PA in AI layer V pyramidal neurons of young mice. The results...