Nostoc flagelliforme is a terrestrial, highly drought-tolerant cyanobacterium. However, the molecular mechanism of its drought adaptation still remains little known. In this paper, we attempted to isolate those osmotic stress-regulated cDNA fragments by traditional differential display reverse transcriptase–polymerase chain reaction (DDRT-PCR). The PCR primers were chosen and evaluated from the reported HIP1D sequence-based primers and Ea/Es primers. The physiologically recovered samples were treated with 0.7 M sorbitol for 3 h. A total of 21 ...
