期刊论文

ANTHER CULTURE OF TRANSGENIC RAPE (Brassica napus L.)

中文

现代牧业

2096-2975

1995

04

1-3+10

国家“863”计划资助项目；

本校为第一机构

本研究通过花药培养技术获得了6个抗Km 20mg/L的单倍体苗,其中花药愈伤组织的最高诱导率是21.6%,最佳激素配比是:Miller+2,4—D 2.0mg/L+KT1.5mg/L+IAA 2.0 mg/L,愈伤组织的苗最高分化率是18.8%,最佳激素配比是:MS+KT2.0mg/L+IAA 0.2mp/L.

In anther culture of transgenic rape plants, six haploid plantlets of being with km-resistahce ( km-r 20mg/L) were obtained. The callus inducing frequency was about 21.6% ( Miller+2, 4-D2.0mg/ L+KT1.5mg/ L+IAA2.0mg/ L) and the plant regeneration frequency was about 18.8% ( MS...