期刊论文

Cloning and Bioinformatic Analysis of nos Cluster Genes from Bacillus cereus

中文

生物资源

2096-3491

2015

37

1

41-48

10.14188/j.ajsh.2015.01.009

国家自然科学基金项目（31101595）； 中央高校基本科研业务费专项资金（120002040）；

本校为第一机构

为了探究革兰氏阳性菌蜡状芽胞杆菌HS-N10 nos基因簇相关基因的特点,PCR扩增获得了HS-N10 nos基因簇相关基因,其中包括nosZ全长1 914 bp、nosR部分片段1 749 bp、nosD部分片段946 bp和nosF部分片段495 bp.经BLASTN分析,HS-N10 nosZ序列和nosD序列分别与施氏假单胞菌(Pseudomonas stutzeri)相应序列相似性最高;HS-N10 nosR序列和nosF序列分别与裂环无色杆菌(Achromobacter c ycloclastes)相应序列相似性最高.通过在线数据库与分析工具分别对HS-N10nos基因簇相关基因进行了生物信息学分析.

To explore the characteristics of nos cluster genes of Bacillus cereus HS-N10,we cloned genes of nos cluster by the method of PCR.The results showed that the sequence of nosZ ORF contained 1 914 bp and the partial nucleotide sequences of nosR,nosDand nosFcontained 1 749 bp,946bp and 495 bp,respectively.By the BLASTIN analysis,nosZ and nosDsequences of HS-N10 were similar to the genes of Pseudomonas stutzeri,while the sequences of nosRand nosF were similar to the genes of Achromobacter cycloclastes.Meanwhile,some bioinformatic analys...