During the development of the anthers,potassium antimonite was used to locate Ca~(2+) in fertile and sterile anthers of purple cytoplasmic male sterile rice (Oryza sativa L.ssp.indica).In the fertile anthers,there was abundant Ca~(2+) precipitates on the surface of early microspore,but not in cytoplasm;In the tapetum,there were little calcium precipitates in cytoplasm in the early stage,in the later stage,tapetum cells suffered programmed cell death and a great quantity of calcium was precipitated.In the sterile anthers,the pollen mother cell aborted at the meiosis stage and abundant calcium w...