期刊论文

中文

昆虫学报

0454-6296

2008

51

9

924-929

10.3321/j.issn:0454-6296.2008.09.005

国家自然科学基金项目(30870350) 教育部科学技术研究重点项目([2006]52:106117)

本校为第一机构

本研究建立并优化了索线虫科(Mermithidae)SAFLP体系,构建了我国常见索线虫科昆虫病原线虫6属11种/亚种的指纹图谱。采用EcoRⅠ和MseⅠ两种限制性内切酶单酶切,结果显示EcoRⅠ酶更适合作为索线虫科线虫 SAFLP的内切酶。3个带有3个选择性碱基的EcoRⅠ引物进行扩增共得条带225个,片段大小为250 ～1 650 bp。通过NTsys-PC2.1软件计算了索线虫科6属11种/亚种的Nei-Li遗传距离(0.1980 ～0.4554)和相似系数,利用NTsys-PC2.1软件中的UPGMA方法构建其聚类图。索线虫科这6属11种/亚种从属级阶元可以分为两大类群:罗索属 Romanomermis和八腱索属Octomyomermis聚为一支构成第一大类群;六索属Hexamermis与卵...

The method of SAFLP(single-enzyme amplified fragment length polymorphism)was optimized for Mermithidae in this study.The genetic relationships among 11 species or subspecies belonging to six genera of Mermithidae and common in China were detected using this method.Two kinds of restriction endonucleases were used in this study,and the results showed that EcoRⅠ was more suitable than MseⅠ for SAFLP analysis of Mermithidae.In total 225 bands(form 250 to 1 65...