期刊论文

中文

昆虫学报

0454-6296

2017

60

11

1292-1299

10.16380/j.kcxb.2017.11.007

国家自然科学基金项目（31672352）；

本校为第一机构

【目的】 DNA甲基化是基因修饰的一种重要方式,主要可以形成5-甲基胞嘧啶(5mC)和6-甲基腺嘌呤(6mA)等。目前关于5mC的研究比较多,而关于6mA在真核生物中的研究则较少。沃尔巴克氏体Wolbachia是昆虫中最常见的共生菌之一,可通过多种方式操纵宿主生殖,其中最常见的就是引起精卵细胞质不亲和(cytoplasmic incompatibility,CI),即感染Wolbachia的雄性与未感染的雌性宿主交配后,胚胎致死,但其机制还不清楚。本研究拟从6mA甲基化的角度,探讨Wolbachia影响果蝇生殖的分子机制。【方法】以模式生物黑腹果蝇Drosophila melanogaster为材料,通过实时荧光定量PCR方法,检测Wolbachia感染对果蝇精巢、卵巢以及...

【Aim】 DNA methylation is one of the important ways of gene modification,mainly resulting in 5mC and 6mA.At present,there have been many studies related to 5mC,while studies on 6mA in eukaryotes are rare.Wolbachia bacteria are endosymbionts frequently found in insects.They can mediate their hosts' reproduction via several strategies.Cytoplasmic incompatibility (CI) is the most common defect induced by Wolbachia,which arrests the development of embryos from uninfected females that are mated with Wolbachia-infected males.The molecular mechanisms...