期刊论文

中文

水生生物学报

1000-3207

2005

29

5

538-543

10.3321/j.issn:1000-3207.2005.05.011

:教育部高校骨干教师资助计划 (995004092G):湖北省武汉市青年科技晨光计划 (2001ABB114):湖北省自然科学基金

本校为第一机构

对罗氏沼虾卵子极体排出时间和第一次卵裂时间进行了观察,并在此基础上,采用热休克和细胞松弛素B(C.B)两种诱导方法成功诱导出了罗氏沼虾四倍体.石蜡切片显示,当罗氏沼虾卵子完全成熟时,第一极体已经排出.第二极体排出的时间约在受精后55min.经过雌性原核和雄性原核的联会,第一次卵裂的时间约在受精后210-230min.设计了多种处理条件,其中热休克诱导罗氏沼虾四倍体的最好条件是:受精后210min,用40℃水温处理胚胎1.5min,在这种条件下,最高可以得到35.86%的四倍体率;而C.B诱导罗氏沼虾四倍体的最好条件是:受精后230min,用1.0mg/L的C.B处理胚胎10min,此时最高四倍体诱导率达33.78%.

Triploid organisms are advantageous in agricultural breeding due to their rapid growth rate and more delicious meat. Tetraploid animals are also valuable because of their fertility and capacity of producing triploidy by crossing with normal diploid. The giant freshwater prawn Macrobrachium rosenbergii is an important aquaculture species in China. The intensive breeding, which is widely used, may cause many problems, such as prematurity, being smaller in size, etc. Triploid induction may be a solution to solve these problems. Present study showe...