期刊论文

STUDIES ON THE PROTECTIVE ROLE OF ZEBRAFISH HO1 IN RESPONSE TO HYPOXIA

中文

水生生物学报

1000-3207

2017

41

1

43-49

10.7541/2017.6

国家自然科学基金项目（30972254） 淡水生态与生物技术国家重点实验室开放课题（2013FB03）资助

本校为第一机构

实验探讨了低氧条件下血红素加氧酶l(Hol)对斑马鱼的保护作用。Real-time PCR结果显示,低氧条件下斑马鱼hol mRNA水平在斑马鱼胚胎和离体培养细胞ZF4中显著增加,而在成鱼的不同组织中呈现不同的反应。低氧处理24h后,斑马鱼脑、鳃和肝脏中hol mRNA表达量明显上升,而在心脏和肾脏中hol mRNA表达量显著降低。用锌原卟啉IX(ZnPPIX)抑制ZF4细胞hol的表达,采用CCK8试剂盒检测细胞存活率,结果显示抑制hol表达可导致低氧条件下ZF4细胞存活率明显降低。利用Hoechst染色和caspase 3活性检测发现,在低氧条件下抑制hol表达后ZF4细胞的凋亡率较对照组显著增加,而Hol的诱导剂可显著降低低氧条件下抑制组的细胞...

b Heme oxygenase 1 （Ho1） is the rate-limiting enzyme in the degradation of heme into biliverdin, carbon monoxide and free divalent iron. Abnormal expression of ho1 gene is associated with the development and progression of various human diseases, while functions of zebrafish Ho1 under hypoxia stress remain largely unknown. This study explored the protection role of zebrafish Ho1 during hypoxia exposure. The results showed that hypoxia significantly induced zebrafish ho1 mRNA in ZF4 cells, embryos, brain, gill, and liver but significantly decr...