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Dual-modal visual/photoelectrochemical all-in-one bioassay for rapid detection of AFP using 3D printed microreactor device

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成果类型:
期刊论文
作者:
Li, Xin;Pan, Xinmeng;Lu, Junmiao;Zhou, Yanping;Gong, Jingming*
通讯作者:
Gong, Jingming
作者机构:
[Zhou, Yanping; Lu, Junmiao; Gong, Jingming; Pan, Xinmeng; Li, Xin] Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Minist Educ,Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
通讯机构:
[Gong, Jingming] C
Cent China Normal Univ, Coll Chem, Int Joint Res Ctr Intelligent Biosensing Technol, Minist Educ,Key Lab Pesticide & Chem Biol, Wuhan 430079, Peoples R China.
语种:
英文
关键词:
3D printed microreactor device;All-in-one bioassay;Dual-modal readout;Fast screening;Rapid quantitative PEC determination
期刊:
Biosensors and Bioelectronics
ISSN:
0956-5663
年:
2020
卷:
158
页码:
112158
基金类别:
This work was supported by National Science Foundation of China (Grant 21475050, 21777052), National Key R&D Program of China (Grant 2018YFC1802003), the Project for Application Foundation Frontier for Wuhan (Grant 2019020701011486), Self-determined Research Funds of CCNU from the Colleges' Basic Research and Operation of MOE (CCNU18TS013), and The Program of Introducing Talents of Discipline to Universities of China (111 program, B17019).
机构署名:
本校为第一且通讯机构
院系归属:
化学学院
摘要:
An elaborated 3D printing "all-in-one" dual-modal immunoassay (3D-AIO) has been constructed for the colorimetric and photoelectrochemical (PEC) detection of alpha-fetoprotein (AFP), which integrates all step-analysis functional components (including immune/enzyme reaction, separation and detection) together using automatic microfluidics. The released ascorbic acid (AA) from the enzyme-linked immunoreactions can induce the aggregation of gold nanoparticles (AuNPs) by reducing cystine into cysteine, serving as the reporting agent of colorimetric assay. Meanwhile, the released AA induces hole-tra...

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